Glycated low density lipoproteins modify platelet properties: A compositional and functional study

G. Ferretti, R. A. Rabini, T. Bacchetti, A. Vignini, E. Salvolini, F. Ravaglia, G. Curatola, L. Mazzanti

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

The interaction between low density lipoproteins (LDL) and platelets might play a central role in the development of atherosclerosis in diabetes. The aim of the present study was to investigate whether the glycation of LDL is associated with modifications of their physico-chemical and functional properties and to study the action of glycated LDL (glycLDL) on platelets. LDL and platelets were isolated from 15 healthy subjects. The content of thiobarbituric acid-reactive substances and the generalized polarization of the fluorescent probe Laurdan were determined in LDL glycated in vitro. Platelets were incubated with native LDL, GlycLDL, and minimally oxidized LDL, and the following parameters were evaluated: platelet aggregation, nitric oxide production, intracellular Ca2+ concentrations, Na+/K+-adenosine triphosphatase (Na+/K+-ATPase), and Ca2+-ATPase activities. GlycLDL showed increased thiobarbituric acid-reactive substance levels, a red shift of the Laurdan emission maximum, and a decrease in generalized polarization, indicating a higher polarity and a reduced molecular order compared with native LDL. GlycLDL caused a significant increase in platelet nitric oxide production, intracellular Ca2+ concentration, and aggregating response to ADP; an inhibition of the platelet membrane Na+/K+-ATPase activity; and a stimulation of Ca2+-ATPase activity. Minimally oxidized LDL did not cause statistically significant changes in the parameters studied. The present work demonstrates that glycation induces compositional and structural changes in LDL and suggests that an altered interaction between glycLDL and platelets might play a role in the vascular complications of diabetes.

Original languageEnglish
Pages (from-to)2180-2184
Number of pages5
JournalJournal of Clinical Endocrinology and Metabolism
Volume87
Issue number5
DOIs
Publication statusPublished - 2002

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Platelets
LDL Lipoproteins
Blood Platelets
Calcium-Transporting ATPases
Thiobarbituric Acid Reactive Substances
Medical problems
Adenosine Triphosphatases
Nitric Oxide
Polarization
Diabetes Complications
Platelet Aggregation
Fluorescent Dyes
Adenosine Diphosphate
Blood Vessels
Atherosclerosis
Healthy Volunteers
Agglomeration
Membranes

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Glycated low density lipoproteins modify platelet properties : A compositional and functional study. / Ferretti, G.; Rabini, R. A.; Bacchetti, T.; Vignini, A.; Salvolini, E.; Ravaglia, F.; Curatola, G.; Mazzanti, L.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 87, No. 5, 2002, p. 2180-2184.

Research output: Contribution to journalArticle

Ferretti, G, Rabini, RA, Bacchetti, T, Vignini, A, Salvolini, E, Ravaglia, F, Curatola, G & Mazzanti, L 2002, 'Glycated low density lipoproteins modify platelet properties: A compositional and functional study', Journal of Clinical Endocrinology and Metabolism, vol. 87, no. 5, pp. 2180-2184. https://doi.org/10.1210/jc.87.5.2180
Ferretti, G. ; Rabini, R. A. ; Bacchetti, T. ; Vignini, A. ; Salvolini, E. ; Ravaglia, F. ; Curatola, G. ; Mazzanti, L. / Glycated low density lipoproteins modify platelet properties : A compositional and functional study. In: Journal of Clinical Endocrinology and Metabolism. 2002 ; Vol. 87, No. 5. pp. 2180-2184.
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