Abstract
Our objective was to investigate the presence of a B and T cell immune response directed against the glycine-rich cell wall protein (GRP) in patients with different autoimmune disorders and with food allergy. GRP is an ubiquitous food protein that has high homology with cytokeratins and other self proteins [Epstein-Barr virus nuclear antigen-1 (EBNA-I), heterogeneous nuclear ribonucleoprotein, fibrillar collagen] which are common targets in autoimmune disorders. A peptide (GGYGDGGAHGGGYGG) derived from GRP was used to screen human sera in direct and competitive ELISA assay. Anti-GRP-specific IgG were analyzed for their ability to cross-react with autoantigens. The intracellular cytokine profiles of the peptide-specific T cell clones obtained from representative patients have been studied. BALB/c mice were immunized with the peptide coupled to the carrier protein keyhole limpet hemocyanin (KLH). Serum IgG antibodies directed against the GRP peptide were detected in several autoimmune disorders and in food allergic patients, and were able to cross-react with autoantigens including keratin, collagen and EBNA-I. Twenty-five T cell clones showed a specific proliferative response to the GRP peptide and were of the T(h)0 phenotype. Eight of the 10 BALB/c mice immunized with the peptide coupled to KLH developed an autoimmune response. Our data suggest that phylogenetically highly conserved epitopes in plants, viruses and humans may be responsible for an autoimmune response in susceptible individuals. They also indicate that the antigen spreading of a particular sequence among apparently divergent proteins may participate to initiate or amplify an immune response.
| Original language | English |
|---|---|
| Pages (from-to) | 647-657 |
| Number of pages | 11 |
| Journal | International Immunology |
| Volume | 12 |
| Issue number | 5 |
| Publication status | Published - 2000 |
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Keywords
- Autoantigens
- Glycine-rich protein
- Random peptide library
ASJC Scopus subject areas
- Immunology
Cite this
Glycine-rich cell wall proteins act as specific antigen targets in autoimmune and food allergic disorders. / Lunardi, Claudio; Nanni, Luca; Tiso, Micaela; Mingari, Maria Cristina; Bason, Caterina; Oliveri, Mara; Keller, Beat; Millo, Romano; De Sandre, Giorgio; Corrocher, Roberto; Puccetti, Antonio.
In: International Immunology, Vol. 12, No. 5, 2000, p. 647-657.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Glycine-rich cell wall proteins act as specific antigen targets in autoimmune and food allergic disorders
AU - Lunardi, Claudio
AU - Nanni, Luca
AU - Tiso, Micaela
AU - Mingari, Maria Cristina
AU - Bason, Caterina
AU - Oliveri, Mara
AU - Keller, Beat
AU - Millo, Romano
AU - De Sandre, Giorgio
AU - Corrocher, Roberto
AU - Puccetti, Antonio
PY - 2000
Y1 - 2000
N2 - Our objective was to investigate the presence of a B and T cell immune response directed against the glycine-rich cell wall protein (GRP) in patients with different autoimmune disorders and with food allergy. GRP is an ubiquitous food protein that has high homology with cytokeratins and other self proteins [Epstein-Barr virus nuclear antigen-1 (EBNA-I), heterogeneous nuclear ribonucleoprotein, fibrillar collagen] which are common targets in autoimmune disorders. A peptide (GGYGDGGAHGGGYGG) derived from GRP was used to screen human sera in direct and competitive ELISA assay. Anti-GRP-specific IgG were analyzed for their ability to cross-react with autoantigens. The intracellular cytokine profiles of the peptide-specific T cell clones obtained from representative patients have been studied. BALB/c mice were immunized with the peptide coupled to the carrier protein keyhole limpet hemocyanin (KLH). Serum IgG antibodies directed against the GRP peptide were detected in several autoimmune disorders and in food allergic patients, and were able to cross-react with autoantigens including keratin, collagen and EBNA-I. Twenty-five T cell clones showed a specific proliferative response to the GRP peptide and were of the T(h)0 phenotype. Eight of the 10 BALB/c mice immunized with the peptide coupled to KLH developed an autoimmune response. Our data suggest that phylogenetically highly conserved epitopes in plants, viruses and humans may be responsible for an autoimmune response in susceptible individuals. They also indicate that the antigen spreading of a particular sequence among apparently divergent proteins may participate to initiate or amplify an immune response.
AB - Our objective was to investigate the presence of a B and T cell immune response directed against the glycine-rich cell wall protein (GRP) in patients with different autoimmune disorders and with food allergy. GRP is an ubiquitous food protein that has high homology with cytokeratins and other self proteins [Epstein-Barr virus nuclear antigen-1 (EBNA-I), heterogeneous nuclear ribonucleoprotein, fibrillar collagen] which are common targets in autoimmune disorders. A peptide (GGYGDGGAHGGGYGG) derived from GRP was used to screen human sera in direct and competitive ELISA assay. Anti-GRP-specific IgG were analyzed for their ability to cross-react with autoantigens. The intracellular cytokine profiles of the peptide-specific T cell clones obtained from representative patients have been studied. BALB/c mice were immunized with the peptide coupled to the carrier protein keyhole limpet hemocyanin (KLH). Serum IgG antibodies directed against the GRP peptide were detected in several autoimmune disorders and in food allergic patients, and were able to cross-react with autoantigens including keratin, collagen and EBNA-I. Twenty-five T cell clones showed a specific proliferative response to the GRP peptide and were of the T(h)0 phenotype. Eight of the 10 BALB/c mice immunized with the peptide coupled to KLH developed an autoimmune response. Our data suggest that phylogenetically highly conserved epitopes in plants, viruses and humans may be responsible for an autoimmune response in susceptible individuals. They also indicate that the antigen spreading of a particular sequence among apparently divergent proteins may participate to initiate or amplify an immune response.
KW - Autoantigens
KW - Glycine-rich protein
KW - Random peptide library
UR - http://www.scopus.com/inward/record.url?scp=0034018701&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034018701&partnerID=8YFLogxK
M3 - Article
C2 - 10784611
AN - SCOPUS:0034018701
VL - 12
SP - 647
EP - 657
JO - International Immunology
JF - International Immunology
SN - 0953-8178
IS - 5
ER -