Glycine-rich cell wall proteins act as specific antigen targets in autoimmune and food allergic disorders

Claudio Lunardi, Luca Nanni, Micaela Tiso, Maria Cristina Mingari, Caterina Bason, Mara Oliveri, Beat Keller, Romano Millo, Giorgio De Sandre, Roberto Corrocher, Antonio Puccetti

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Our objective was to investigate the presence of a B and T cell immune response directed against the glycine-rich cell wall protein (GRP) in patients with different autoimmune disorders and with food allergy. GRP is an ubiquitous food protein that has high homology with cytokeratins and other self proteins [Epstein-Barr virus nuclear antigen-1 (EBNA-I), heterogeneous nuclear ribonucleoprotein, fibrillar collagen] which are common targets in autoimmune disorders. A peptide (GGYGDGGAHGGGYGG) derived from GRP was used to screen human sera in direct and competitive ELISA assay. Anti-GRP-specific IgG were analyzed for their ability to cross-react with autoantigens. The intracellular cytokine profiles of the peptide-specific T cell clones obtained from representative patients have been studied. BALB/c mice were immunized with the peptide coupled to the carrier protein keyhole limpet hemocyanin (KLH). Serum IgG antibodies directed against the GRP peptide were detected in several autoimmune disorders and in food allergic patients, and were able to cross-react with autoantigens including keratin, collagen and EBNA-I. Twenty-five T cell clones showed a specific proliferative response to the GRP peptide and were of the T(h)0 phenotype. Eight of the 10 BALB/c mice immunized with the peptide coupled to KLH developed an autoimmune response. Our data suggest that phylogenetically highly conserved epitopes in plants, viruses and humans may be responsible for an autoimmune response in susceptible individuals. They also indicate that the antigen spreading of a particular sequence among apparently divergent proteins may participate to initiate or amplify an immune response.

Original languageEnglish
Pages (from-to)647-657
Number of pages11
JournalInternational Immunology
Volume12
Issue number5
Publication statusPublished - 2000

Fingerprint

Glycine
Cell Wall
Peptide T
Antigens
Food
Peptides
Autoantigens
Keratins
Autoimmunity
T-Lymphocytes
Proteins
Clone Cells
Immunoglobulin G
Epstein-Barr Virus Nuclear Antigens
Fibrillar Collagens
Heterogeneous-Nuclear Ribonucleoproteins
Patient Advocacy
Plant Viruses
Food Hypersensitivity
Serum

Keywords

  • Autoantigens
  • Glycine-rich protein
  • Random peptide library

ASJC Scopus subject areas

  • Immunology

Cite this

Glycine-rich cell wall proteins act as specific antigen targets in autoimmune and food allergic disorders. / Lunardi, Claudio; Nanni, Luca; Tiso, Micaela; Mingari, Maria Cristina; Bason, Caterina; Oliveri, Mara; Keller, Beat; Millo, Romano; De Sandre, Giorgio; Corrocher, Roberto; Puccetti, Antonio.

In: International Immunology, Vol. 12, No. 5, 2000, p. 647-657.

Research output: Contribution to journalArticle

Lunardi, C, Nanni, L, Tiso, M, Mingari, MC, Bason, C, Oliveri, M, Keller, B, Millo, R, De Sandre, G, Corrocher, R & Puccetti, A 2000, 'Glycine-rich cell wall proteins act as specific antigen targets in autoimmune and food allergic disorders', International Immunology, vol. 12, no. 5, pp. 647-657.
Lunardi, Claudio ; Nanni, Luca ; Tiso, Micaela ; Mingari, Maria Cristina ; Bason, Caterina ; Oliveri, Mara ; Keller, Beat ; Millo, Romano ; De Sandre, Giorgio ; Corrocher, Roberto ; Puccetti, Antonio. / Glycine-rich cell wall proteins act as specific antigen targets in autoimmune and food allergic disorders. In: International Immunology. 2000 ; Vol. 12, No. 5. pp. 647-657.
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