Glycine-spacers influence functional motifs exposure and self-assembling propensity of functionalized substrates tailored for neural stem cell cultures

Francesca Taraballi, Antonino Natalello, Marcello Campione, Omar Villa, Silvia M. Doglia, Alberto Paleari, Fabrizio Gelain

Research output: Contribution to journalArticle


The understanding of phenomena involved in the self-assembling of bio-inspired biomaterials acting as three-dimensional scaffolds for regenerative medicine applications is a necessary step to develop effective therapies in neural tissue engineering. We investigated the self-assembled nanostructures of functionalized peptides featuring four, two or no glycine-spacers between the self-assembly sequence RADA16-I and the functional biological motif PFSSTKT. The effectiveness of their biological functionalization was assessed via in vitro experiments with neural stem cells (NSCs) and their molecular assembly was elucidated via atomic force microscopy, Raman and Fourier Transform Infrared spectroscopy. We demonstrated that glycine-spacers play a crucial role in the scaffold stability and in the exposure of the functional motifs. In particular, a glycinespacer of four residues leads to a more stable nanostructure and to an improved exposure of the functional motif. Accordingly, the longer spacer of glycines, the more effective is the functional motif in both eliciting NSCs adhesion, improving their viability and increasing their differentiation. Therefore, optimized designing strategies of functionalized biomaterials may open, in the near future, new therapies in tissue engineering and regenerative medicine.

Original languageEnglish
Article number1
JournalFrontiers in Neuroengineering
Issue numberFEB
Publication statusPublished - Feb 8 2010



  • AFM
  • Biomaterial
  • FTIR
  • Micro-Raman
  • Nanostructure
  • Neural stem cell

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biophysics
  • Neuroscience (miscellaneous)

Cite this