Glycogenolysis in astrocytes supports blood-borne glucose channeling not glycogen-derived lactate shuttling to neurons: Evidence from mathematical modeling

Mauro Dinuzzo, Silvia Mangia, Bruno Maraviglia, Federico Giove

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

In this article, we examined theoretically the role of human cerebral glycogen in buffering the metabolic requirement of a 360-second brain stimulation, expanding our previous modeling study of neurometabolic coupling. We found that glycogen synthesis and degradation affects the relative amount of glucose taken up by neurons versus astrocytes. Under conditions of 175:115 mmol/L (∼1.5:1) neuronal versus astrocytic activation-induced Na influx ratio, ∼12% of astrocytic glycogen is mobilized. This results in the rapid increase of intracellular glucose-6-phosphate level on stimulation and nearly 40% mean decrease of glucose flow through hexokinase (HK) in astrocytes via product inhibition. The suppression of astrocytic glucose phosphorylation, in turn, favors the channeling of glucose from interstitium to nearby activated neurons, without a critical effect on the concurrent intercellular lactate trafficking. Under conditions of increased neuronal versus astrocytic activation-induced Na influx ratio to 190:65 mmol/L (∼3:1), glycogen is not significantly degraded and blood glucose is primarily taken up by neurons. These results support a role for astrocytic glycogen in preserving extracellular glucose for neuronal utilization, rather than providing lactate to neurons as is commonly accepted by the current thinking paradigm. This might be critical in subcellular domains during functional conditions associated with fast energetic demands.

Original languageEnglish
Pages (from-to)1895-1904
Number of pages10
JournalJournal of Cerebral Blood Flow and Metabolism
Volume30
Issue number12
DOIs
Publication statusPublished - Dec 2010

Fingerprint

Glycogenolysis
Glycogen
Astrocytes
Blood Glucose
Lactic Acid
Neurons
Glucose
Glucose-6-Phosphate
Hexokinase
Phosphorylation
Brain

Keywords

  • brain glycogen
  • lactate shuttle
  • mathematical modeling
  • neurometabolic coupling
  • neuronal activation

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Clinical Neurology
  • Neurology

Cite this

Glycogenolysis in astrocytes supports blood-borne glucose channeling not glycogen-derived lactate shuttling to neurons : Evidence from mathematical modeling. / Dinuzzo, Mauro; Mangia, Silvia; Maraviglia, Bruno; Giove, Federico.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 30, No. 12, 12.2010, p. 1895-1904.

Research output: Contribution to journalArticle

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