Glycolysis and growth rate in normal and in hexokinase-transfected NIH-3T3 cells

Maurizio Fanciulli, Marco G. Paggi, Tiziana Bruno, Carlo Del Carlo, Francesco Bonetto, Francesco P. Gentile, Aristide Floridi

Research output: Contribution to journalArticlepeer-review

Abstract

The glycolytic enzyme hexokinase plays a key role in regulating cell energy metabolism. Its activity has been associated with cell growth rate and, notably, with neoplastic transformation. NIH-3T3 cells were transfected with a tumor hexokinase cDNA. The transfected cells showed increased hexokinase amount and activity, mainly located in the particulate cellular fraction, increased glycolytic rate evaluated as lactate production, and, finally, enhanced growth rate. These data may suggest that high hexokinase activity might be not merely the consequence of peculiar metabolic demands by actively replicating normal or cancer cells, but also a modification able per se to drive, at least partially, a more intense mitotic activity.

Original languageEnglish
Pages (from-to)405-409
Number of pages5
JournalOncology Research
Volume6
Issue number9
Publication statusPublished - 1994

Keywords

  • growth rate
  • hexokinase
  • NIH-3T3 cells
  • transfection

ASJC Scopus subject areas

  • Cancer Research

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