Glycosylated proteins as an indicator of metabolic control in diabetes: evaluation by aminophenylboronic acid affinity chromatography.

A. Ceriello, P. dello Russo, F. Curcio, G. Paolisso, G. Illiano, S. Sgambato, D. Giugliano

Research output: Contribution to journalArticle

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Abstract

Glycosylated proteins, glycosylated albumin and HbA1 were studied in type I and type II diabetes. Glycosylated proteins were evaluated by a new method: aminophenylboronic acid affinity chromatography. A good correlation was found between total HbA1 and glycosylated proteins in both groups (r = 0.57, p less than 0.05; r = 0.67, p less than 0.01, respectively), but a positive correlation between stable HbA1 and glycosylated proteins was present only in maturity onset diabetics (r = 0.71, p less than 0.01). Glycosylated proteins correlated with glycosylated albumin only in type II diabetes (r = 0.67, p less than 0.01). We hypothesize that in maturity onset diabetes glycosylated proteins and HbA1 reflect a greater glycemic stability while in insulin-dependent diabetes the same proteins reflect a different periodic pattern of glycemic control. Moreover, our data suggest that aminophenylboronic acid affinity chromatography is a suitable tool for routine monitoring of metabolic control in maturity onset diabetes, while further investigations are needed to establish if this method is a useful index of altered glucose metabolism in type I diabetes.

Original languageEnglish
Pages (from-to)49-54
Number of pages6
JournalActa Diabetologica Latina
Volume21
Issue number1
Publication statusPublished - Jan 1984

Fingerprint

Affinity Chromatography
Glycosylated Hemoglobin A
Type 2 Diabetes Mellitus
Proteins
Albumins
3-aminobenzeneboronic acid
Type 1 Diabetes Mellitus
Insulin
Glucose

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Internal Medicine

Cite this

Glycosylated proteins as an indicator of metabolic control in diabetes : evaluation by aminophenylboronic acid affinity chromatography. / Ceriello, A.; dello Russo, P.; Curcio, F.; Paolisso, G.; Illiano, G.; Sgambato, S.; Giugliano, D.

In: Acta Diabetologica Latina, Vol. 21, No. 1, 01.1984, p. 49-54.

Research output: Contribution to journalArticle

Ceriello, A, dello Russo, P, Curcio, F, Paolisso, G, Illiano, G, Sgambato, S & Giugliano, D 1984, 'Glycosylated proteins as an indicator of metabolic control in diabetes: evaluation by aminophenylboronic acid affinity chromatography.', Acta Diabetologica Latina, vol. 21, no. 1, pp. 49-54.
Ceriello, A. ; dello Russo, P. ; Curcio, F. ; Paolisso, G. ; Illiano, G. ; Sgambato, S. ; Giugliano, D. / Glycosylated proteins as an indicator of metabolic control in diabetes : evaluation by aminophenylboronic acid affinity chromatography. In: Acta Diabetologica Latina. 1984 ; Vol. 21, No. 1. pp. 49-54.
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