GMP-grade platelet lysate enhances proliferation and migration of tenon fibroblasts

Augusto Carducci, Gaia Scafetta, Camilla Siciliano, Roberto Carnevale, Paolo Rosa, Andrea Coccia, Giorgio Mangino, Antonella Bordin, Enzo Maria Vingolo, Luca Pierelli, Eugenio Lendaro, Giuseppe Ragona, Giacomo Frati, Elena De Falco

Research output: Contribution to journalArticle

Abstract

Tenon's fibroblasts (TFs), widely employed as in vitro model for many ophthalmological studies, are routinely cultured with FBS. Platelet Lysate (PL), a hemoderivate enriched with growth factors and cytokines has been largely tested in several clinical applications and as substitute of FBS in culture. Here, we investigate whether PL can exert biological effects on TF populations similarly to other cell types. Results show that PL significantly enhances cell proliferation and migration vs. FBS, without influencing cell size/granularity. Upregulation of EGF, VEGF, KDR, MMP2-9, FAK mRNA levels also occurs and phosphorylation of AKT but not of ERK1/2 is significantly enhanced. The inhibition of the PI3kinase/AKT pathway with the specific inhibitor wortmannin, decreases PL-induced cell migration but not proliferation. Condition supernatants containing PL show increased bioavailability of Nitric Oxide and reduced levels of 8-Iso-PGF2-alpha, correlating with cell proliferation and migration. Pro-angiogenic/inflammatory soluble factors (GRO, Angiogenin, EGF, I-309, PARC) are exclusively or greater expressed in media containing PL than FBS. GMP-grade PL preparations positively influence in vitro biological effects of TFs representing a suitable and safer alternative to FBS.

Original languageEnglish
Pages (from-to)84-99
Number of pages16
JournalFrontiers in Bioscience - Elite
Volume8
Publication statusPublished - Jan 1 2016

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Keywords

  • Blood Platelets
  • Cell Movement
  • Cell Proliferation
  • Cell Size
  • Culture Media, Conditioned
  • Fibroblasts
  • Humans
  • Tenon Capsule
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

Carducci, A., Scafetta, G., Siciliano, C., Carnevale, R., Rosa, P., Coccia, A., Mangino, G., Bordin, A., Vingolo, E. M., Pierelli, L., Lendaro, E., Ragona, G., Frati, G., & De Falco, E. (2016). GMP-grade platelet lysate enhances proliferation and migration of tenon fibroblasts. Frontiers in Bioscience - Elite, 8, 84-99.