Goat immunoglobulin purification on phosphocellulose and deae affigel blue.

P. Ninfali, L. Baronciani, S. Rapa, D. Marzioni, F. Mannello

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We describe a method for the efficient purification of immunoglobulins G (IgG)to near homogeneity from goat serum. This was achieved by performing first an AS-40 fractionation on goat serum, followed by chromatography on phosphocellulose (P11) equilibrated in citrate buffer at pH 5.7. Peak I, eluted at V0from P11, contained all IgG and the other serum proteins,except β -globulins and most of the a -2- globulins, which are eluted in a second peak with 0.24 M K-phosphate in citrate buffer at pH 6.0. Peak I, concentrated and dialyzed in 20 mM K-phosphate buffer pH 8.0, was then applied onto a DEAE Affi-Gel Blue column equilibrated in the same buffer. Two peaks were obtained from this column: peak I, eluted at V0contained a pure IgG fraction,while the other serum proteins were in peak II.We conclude that the P11 step performed under the conditions we report here,is very useful to retain the α- 2 and β-globulins, which contaminate the IgG when only the DEAE Affi-Gel Blue purification step is used.

Original languageEnglish
Pages (from-to)1-13
Number of pages13
JournalPreparative Biochemistry
Volume24
Issue number1
DOIs
Publication statusPublished - Feb 1 1994

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Goats
Purification
Immunoglobulins
Globulins
Buffers
Immunoglobulin G
Citric Acid
Blood Proteins
Phosphates
Fractionation
Chromatography
Serum
phosphocellulose
DEAE affigel blue

ASJC Scopus subject areas

  • Biochemistry
  • Genetics

Cite this

Goat immunoglobulin purification on phosphocellulose and deae affigel blue. / Ninfali, P.; Baronciani, L.; Rapa, S.; Marzioni, D.; Mannello, F.

In: Preparative Biochemistry, Vol. 24, No. 1, 01.02.1994, p. 1-13.

Research output: Contribution to journalArticle

Ninfali, P. ; Baronciani, L. ; Rapa, S. ; Marzioni, D. ; Mannello, F. / Goat immunoglobulin purification on phosphocellulose and deae affigel blue. In: Preparative Biochemistry. 1994 ; Vol. 24, No. 1. pp. 1-13.
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