Gold branched nanoparticles for cellular treatments

Laura Sironi, Stefano Freddi, Michele Caccia, Paolo Pozzi, Leone Rossetti, Piersandro Pallavicini, Alice Donà, Elisa Cabrini, Maurizio Gualtieri, Ilaria Rivolta, Alice Panariti, Laura Dalfonso, Maddalena Collini, Giuseppe Chirico

Research output: Contribution to journalArticle

Abstract

Under the action of near-infrared radiation, shape anisotropic gold nanoparticles emit two-photon luminescence and release heat. Accordingly, they have been proposed for imaging, photothermal therapies and thermo-controlled drug delivery. In all these applications particular care must be given to control the nanoparticle - cell interaction and the thermal efficiency of the nanoparticles, while minimizing their intrinsic cytotoxicity. We present here the characterization of the cell interaction of newly developed branched gold nanostars, obtained by laurylsulfobetaine-driven seed-growth synthesis. The study provides information on the size distribution, the shape anisotropy, the cellular uptake and cytotoxicity of the gold nanostars as well as their intracellular dynamic behavior by means of two-photon luminescence imaging, fluorescence correlation spectroscopy and particle tracking. The results show that the gold nanostars are internalized as well as the widely used gold nanorods and are less toxic under prolonged treatments. At the same time they display remarkable two-photon luminescence and large extinction under polarized light in the near-infrared region of the spectrum, 800-950 nm. Gold nanostars appear then a valuable alternative to other elongated or in-homogeneous nanoparticles for cell imaging.

Original languageEnglish
Pages (from-to)18407-18418
Number of pages12
JournalJournal of Physical Chemistry C
Volume116
Issue number34
DOIs
Publication statusPublished - Aug 30 2012

Fingerprint

Gold
gold
Nanoparticles
nanoparticles
Luminescence
Photons
Cytotoxicity
luminescence
Imaging techniques
photons
cells
near infrared radiation
Controlled drug delivery
Infrared radiation
Light extinction
thermodynamic efficiency
Poisons
Light polarization
Nanorods
polarized light

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Electronic, Optical and Magnetic Materials
  • Surfaces, Coatings and Films
  • Energy(all)

Cite this

Sironi, L., Freddi, S., Caccia, M., Pozzi, P., Rossetti, L., Pallavicini, P., ... Chirico, G. (2012). Gold branched nanoparticles for cellular treatments. Journal of Physical Chemistry C, 116(34), 18407-18418. https://doi.org/10.1021/jp305021k

Gold branched nanoparticles for cellular treatments. / Sironi, Laura; Freddi, Stefano; Caccia, Michele; Pozzi, Paolo; Rossetti, Leone; Pallavicini, Piersandro; Donà, Alice; Cabrini, Elisa; Gualtieri, Maurizio; Rivolta, Ilaria; Panariti, Alice; Dalfonso, Laura; Collini, Maddalena; Chirico, Giuseppe.

In: Journal of Physical Chemistry C, Vol. 116, No. 34, 30.08.2012, p. 18407-18418.

Research output: Contribution to journalArticle

Sironi, L, Freddi, S, Caccia, M, Pozzi, P, Rossetti, L, Pallavicini, P, Donà, A, Cabrini, E, Gualtieri, M, Rivolta, I, Panariti, A, Dalfonso, L, Collini, M & Chirico, G 2012, 'Gold branched nanoparticles for cellular treatments', Journal of Physical Chemistry C, vol. 116, no. 34, pp. 18407-18418. https://doi.org/10.1021/jp305021k
Sironi L, Freddi S, Caccia M, Pozzi P, Rossetti L, Pallavicini P et al. Gold branched nanoparticles for cellular treatments. Journal of Physical Chemistry C. 2012 Aug 30;116(34):18407-18418. https://doi.org/10.1021/jp305021k
Sironi, Laura ; Freddi, Stefano ; Caccia, Michele ; Pozzi, Paolo ; Rossetti, Leone ; Pallavicini, Piersandro ; Donà, Alice ; Cabrini, Elisa ; Gualtieri, Maurizio ; Rivolta, Ilaria ; Panariti, Alice ; Dalfonso, Laura ; Collini, Maddalena ; Chirico, Giuseppe. / Gold branched nanoparticles for cellular treatments. In: Journal of Physical Chemistry C. 2012 ; Vol. 116, No. 34. pp. 18407-18418.
@article{a62158cdd766445fb0f6f1e343ca2eac,
title = "Gold branched nanoparticles for cellular treatments",
abstract = "Under the action of near-infrared radiation, shape anisotropic gold nanoparticles emit two-photon luminescence and release heat. Accordingly, they have been proposed for imaging, photothermal therapies and thermo-controlled drug delivery. In all these applications particular care must be given to control the nanoparticle - cell interaction and the thermal efficiency of the nanoparticles, while minimizing their intrinsic cytotoxicity. We present here the characterization of the cell interaction of newly developed branched gold nanostars, obtained by laurylsulfobetaine-driven seed-growth synthesis. The study provides information on the size distribution, the shape anisotropy, the cellular uptake and cytotoxicity of the gold nanostars as well as their intracellular dynamic behavior by means of two-photon luminescence imaging, fluorescence correlation spectroscopy and particle tracking. The results show that the gold nanostars are internalized as well as the widely used gold nanorods and are less toxic under prolonged treatments. At the same time they display remarkable two-photon luminescence and large extinction under polarized light in the near-infrared region of the spectrum, 800-950 nm. Gold nanostars appear then a valuable alternative to other elongated or in-homogeneous nanoparticles for cell imaging.",
author = "Laura Sironi and Stefano Freddi and Michele Caccia and Paolo Pozzi and Leone Rossetti and Piersandro Pallavicini and Alice Don{\`a} and Elisa Cabrini and Maurizio Gualtieri and Ilaria Rivolta and Alice Panariti and Laura Dalfonso and Maddalena Collini and Giuseppe Chirico",
year = "2012",
month = "8",
day = "30",
doi = "10.1021/jp305021k",
language = "English",
volume = "116",
pages = "18407--18418",
journal = "Journal of Physical Chemistry C",
issn = "1932-7447",
publisher = "American Chemical Society",
number = "34",

}

TY - JOUR

T1 - Gold branched nanoparticles for cellular treatments

AU - Sironi, Laura

AU - Freddi, Stefano

AU - Caccia, Michele

AU - Pozzi, Paolo

AU - Rossetti, Leone

AU - Pallavicini, Piersandro

AU - Donà, Alice

AU - Cabrini, Elisa

AU - Gualtieri, Maurizio

AU - Rivolta, Ilaria

AU - Panariti, Alice

AU - Dalfonso, Laura

AU - Collini, Maddalena

AU - Chirico, Giuseppe

PY - 2012/8/30

Y1 - 2012/8/30

N2 - Under the action of near-infrared radiation, shape anisotropic gold nanoparticles emit two-photon luminescence and release heat. Accordingly, they have been proposed for imaging, photothermal therapies and thermo-controlled drug delivery. In all these applications particular care must be given to control the nanoparticle - cell interaction and the thermal efficiency of the nanoparticles, while minimizing their intrinsic cytotoxicity. We present here the characterization of the cell interaction of newly developed branched gold nanostars, obtained by laurylsulfobetaine-driven seed-growth synthesis. The study provides information on the size distribution, the shape anisotropy, the cellular uptake and cytotoxicity of the gold nanostars as well as their intracellular dynamic behavior by means of two-photon luminescence imaging, fluorescence correlation spectroscopy and particle tracking. The results show that the gold nanostars are internalized as well as the widely used gold nanorods and are less toxic under prolonged treatments. At the same time they display remarkable two-photon luminescence and large extinction under polarized light in the near-infrared region of the spectrum, 800-950 nm. Gold nanostars appear then a valuable alternative to other elongated or in-homogeneous nanoparticles for cell imaging.

AB - Under the action of near-infrared radiation, shape anisotropic gold nanoparticles emit two-photon luminescence and release heat. Accordingly, they have been proposed for imaging, photothermal therapies and thermo-controlled drug delivery. In all these applications particular care must be given to control the nanoparticle - cell interaction and the thermal efficiency of the nanoparticles, while minimizing their intrinsic cytotoxicity. We present here the characterization of the cell interaction of newly developed branched gold nanostars, obtained by laurylsulfobetaine-driven seed-growth synthesis. The study provides information on the size distribution, the shape anisotropy, the cellular uptake and cytotoxicity of the gold nanostars as well as their intracellular dynamic behavior by means of two-photon luminescence imaging, fluorescence correlation spectroscopy and particle tracking. The results show that the gold nanostars are internalized as well as the widely used gold nanorods and are less toxic under prolonged treatments. At the same time they display remarkable two-photon luminescence and large extinction under polarized light in the near-infrared region of the spectrum, 800-950 nm. Gold nanostars appear then a valuable alternative to other elongated or in-homogeneous nanoparticles for cell imaging.

UR - http://www.scopus.com/inward/record.url?scp=84865740815&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84865740815&partnerID=8YFLogxK

U2 - 10.1021/jp305021k

DO - 10.1021/jp305021k

M3 - Article

AN - SCOPUS:84865740815

VL - 116

SP - 18407

EP - 18418

JO - Journal of Physical Chemistry C

JF - Journal of Physical Chemistry C

SN - 1932-7447

IS - 34

ER -