Gold-nanoparticle-based colorimetric discrimination of cancer-related point mutations with picomolar sensitivity

Paola Valentini, Roberto Fiammengo, Stefania Sabella, Manuela Gariboldi, Gabriele Maiorano, Roberto Cingolani, Pier Paolo Pompa

Research output: Contribution to journalArticlepeer-review

Abstract

Point mutations in the Kirsten rat sarcoma viral oncogene homologue (KRAS) gene are being increasingly recognized as important diagnostic and prognostic markers in cancer. In this work, we describe a rapid and low-cost method for the naked-eye detection of cancer-related point mutations in KRAS based on gold nanoparticles. This simple colorimetric assay is sensitive (limit of detection in the low picomolar range), instrument-free, and employs nonstringent room temperature conditions due to a combination of DNA-conjugated gold nanoparticles, a probe design which exploits cooperative hybridization for increased binding affinity, and signal enhancement on the surface of magnetic beads. Additionally, the scheme is suitable for point-of-care applications, as it combines naked-eye detection, small sample volumes, and isothermal (PCR-free) amplification.

Original languageEnglish
Pages (from-to)5530-5538
Number of pages9
JournalACS Nano
Volume7
Issue number6
DOIs
Publication statusPublished - Jun 25 2013

Keywords

  • biosensors
  • colorimetric
  • genotyping
  • gold nanoparticles
  • stacking interactions

ASJC Scopus subject areas

  • Engineering(all)
  • Materials Science(all)
  • Physics and Astronomy(all)

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