Golgi membrane fission requires the CtBP1-S/BARS-induced activation of lysophosphatidic acid acyltransferase δ

Alessandro Pagliuso; Carmen Valente; Lucia Laura Giordano; Angela Filograna; Guiling Li; Diego Circolo; Gabriele Turacchio; Vincenzo Manuel Marzullo; Luigi Mandrich; Mikhail A. Zhukovsky; Fabio Formiggini; Roman S. Polishchuk; Daniela Corda; Alberto Luini

doi:10.1038/ncomms12148

Golgi membrane fission requires the CtBP1-S/BARS-induced activation of lysophosphatidic acid acyltransferase δ

Alessandro Pagliuso, Carmen Valente, Lucia Laura Giordano, Angela Filograna, Guiling Li, Diego Circolo, Gabriele Turacchio, Vincenzo Manuel Marzullo, Luigi Mandrich, Mikhail A. Zhukovsky, Fabio Formiggini, Roman S. Polishchuk, Daniela Corda, Alberto Luini

IRCCS SDN

Research output: Contribution to journal › Article › peer-review

Abstract

Membrane fission is an essential cellular process by which continuous membranes split into separate parts. We have previously identified CtBP1-S/BARS (BARS) as a key component of a protein complex that is required for fission of several endomembranes, including basolateral post-Golgi transport carriers. Assembly of this complex occurs at the Golgi apparatus, where BARS binds to the phosphoinositide kinase PI4KIIIβ through a 14-3-3γ dimer, as well as to ARF and the PKD and PAK kinases.We now report that, when incorporated into this complex, BARS binds to and activates a trans-Golgi lysophosphatidic acid (LPA) acyltransferase type δ(LPAATδ) that converts LPA into phosphatidic acid (PA); and that this reaction is essential for fission of the carriers. LPA and PA have unique biophysical properties, and their interconversion might facilitate the fission process either directly or indirectly (via recruitment of proteins that bind to PA, including BARS itself).

Original language	English
Article number	12148
Journal	Nature Communications
Volume	7
DOIs	https://doi.org/10.1038/ncomms12148
Publication status	Published - Jul 12 2016

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Chemistry(all)
Physics and Astronomy(all)

Access to Document

10.1038/ncomms12148

Cite this

Pagliuso, A., Valente, C., Giordano, L. L., Filograna, A., Li, G., Circolo, D., Turacchio, G., Marzullo, V. M., Mandrich, L., Zhukovsky, M. A., Formiggini, F., Polishchuk, R. S., Corda, D., & Luini, A. (2016). Golgi membrane fission requires the CtBP1-S/BARS-induced activation of lysophosphatidic acid acyltransferase δ. Nature Communications, 7, [12148]. https://doi.org/10.1038/ncomms12148

Golgi membrane fission requires the CtBP1-S/BARS-induced activation of lysophosphatidic acid acyltransferase δ. / Pagliuso, Alessandro; Valente, Carmen; Giordano, Lucia Laura; Filograna, Angela; Li, Guiling; Circolo, Diego; Turacchio, Gabriele; Marzullo, Vincenzo Manuel; Mandrich, Luigi; Zhukovsky, Mikhail A.; Formiggini, Fabio; Polishchuk, Roman S.; Corda, Daniela; Luini, Alberto.

In: Nature Communications, Vol. 7, 12148, 12.07.2016.

Research output: Contribution to journal › Article › peer-review

Pagliuso, A, Valente, C, Giordano, LL, Filograna, A, Li, G, Circolo, D, Turacchio, G, Marzullo, VM, Mandrich, L, Zhukovsky, MA, Formiggini, F, Polishchuk, RS, Corda, D & Luini, A 2016, 'Golgi membrane fission requires the CtBP1-S/BARS-induced activation of lysophosphatidic acid acyltransferase δ', Nature Communications, vol. 7, 12148. https://doi.org/10.1038/ncomms12148

Pagliuso, Alessandro ; Valente, Carmen ; Giordano, Lucia Laura ; Filograna, Angela ; Li, Guiling ; Circolo, Diego ; Turacchio, Gabriele ; Marzullo, Vincenzo Manuel ; Mandrich, Luigi ; Zhukovsky, Mikhail A. ; Formiggini, Fabio ; Polishchuk, Roman S. ; Corda, Daniela ; Luini, Alberto. / Golgi membrane fission requires the CtBP1-S/BARS-induced activation of lysophosphatidic acid acyltransferase δ. In: Nature Communications. 2016 ; Vol. 7.

@article{45deb6d8d490490b85a716a66d14aa57,

title = "Golgi membrane fission requires the CtBP1-S/BARS-induced activation of lysophosphatidic acid acyltransferase δ",

abstract = "Membrane fission is an essential cellular process by which continuous membranes split into separate parts. We have previously identified CtBP1-S/BARS (BARS) as a key component of a protein complex that is required for fission of several endomembranes, including basolateral post-Golgi transport carriers. Assembly of this complex occurs at the Golgi apparatus, where BARS binds to the phosphoinositide kinase PI4KIIIβ through a 14-3-3γ dimer, as well as to ARF and the PKD and PAK kinases.We now report that, when incorporated into this complex, BARS binds to and activates a trans-Golgi lysophosphatidic acid (LPA) acyltransferase type δ(LPAATδ) that converts LPA into phosphatidic acid (PA); and that this reaction is essential for fission of the carriers. LPA and PA have unique biophysical properties, and their interconversion might facilitate the fission process either directly or indirectly (via recruitment of proteins that bind to PA, including BARS itself).",

author = "Alessandro Pagliuso and Carmen Valente and Giordano, {Lucia Laura} and Angela Filograna and Guiling Li and Diego Circolo and Gabriele Turacchio and Marzullo, {Vincenzo Manuel} and Luigi Mandrich and Zhukovsky, {Mikhail A.} and Fabio Formiggini and Polishchuk, {Roman S.} and Daniela Corda and Alberto Luini",

year = "2016",

month = jul,

day = "12",

doi = "10.1038/ncomms12148",

language = "English",

volume = "7",

journal = "Nature Communications",

issn = "2041-1723",

publisher = "NLM (Medline)",

}

TY - JOUR

T1 - Golgi membrane fission requires the CtBP1-S/BARS-induced activation of lysophosphatidic acid acyltransferase δ

AU - Pagliuso, Alessandro

AU - Valente, Carmen

AU - Giordano, Lucia Laura

AU - Filograna, Angela

AU - Li, Guiling

AU - Circolo, Diego

AU - Turacchio, Gabriele

AU - Marzullo, Vincenzo Manuel

AU - Mandrich, Luigi

AU - Zhukovsky, Mikhail A.

AU - Formiggini, Fabio

AU - Polishchuk, Roman S.

AU - Corda, Daniela

AU - Luini, Alberto

PY - 2016/7/12

Y1 - 2016/7/12

N2 - Membrane fission is an essential cellular process by which continuous membranes split into separate parts. We have previously identified CtBP1-S/BARS (BARS) as a key component of a protein complex that is required for fission of several endomembranes, including basolateral post-Golgi transport carriers. Assembly of this complex occurs at the Golgi apparatus, where BARS binds to the phosphoinositide kinase PI4KIIIβ through a 14-3-3γ dimer, as well as to ARF and the PKD and PAK kinases.We now report that, when incorporated into this complex, BARS binds to and activates a trans-Golgi lysophosphatidic acid (LPA) acyltransferase type δ(LPAATδ) that converts LPA into phosphatidic acid (PA); and that this reaction is essential for fission of the carriers. LPA and PA have unique biophysical properties, and their interconversion might facilitate the fission process either directly or indirectly (via recruitment of proteins that bind to PA, including BARS itself).

AB - Membrane fission is an essential cellular process by which continuous membranes split into separate parts. We have previously identified CtBP1-S/BARS (BARS) as a key component of a protein complex that is required for fission of several endomembranes, including basolateral post-Golgi transport carriers. Assembly of this complex occurs at the Golgi apparatus, where BARS binds to the phosphoinositide kinase PI4KIIIβ through a 14-3-3γ dimer, as well as to ARF and the PKD and PAK kinases.We now report that, when incorporated into this complex, BARS binds to and activates a trans-Golgi lysophosphatidic acid (LPA) acyltransferase type δ(LPAATδ) that converts LPA into phosphatidic acid (PA); and that this reaction is essential for fission of the carriers. LPA and PA have unique biophysical properties, and their interconversion might facilitate the fission process either directly or indirectly (via recruitment of proteins that bind to PA, including BARS itself).

UR - http://www.scopus.com/inward/record.url?scp=84978877476&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84978877476&partnerID=8YFLogxK

U2 - 10.1038/ncomms12148

DO - 10.1038/ncomms12148

M3 - Article

AN - SCOPUS:84978877476

VL - 7

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 12148

ER -

Golgi membrane fission requires the CtBP1-S/BARS-induced activation of lysophosphatidic acid acyltransferase δ

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this