The present study evaluated the presence of GnRH-R in leiomyomas, in associated, non-involved uterine tissues (myometrium and endometrium) and the possible relationships between GnRH-R and the receptors for estrogen and progesterone in the same tissues. GnRH-R was found in all uterine tissues and both GnRH and the GnRH analog, goserelin, displaced its binding consistent with a single type of high affinity receptor (Kd≃10-8 M). GnRH-R were found more frequently in myometrium (81% of samples) than in endometrium (58%) or leiomyoma (42%). However, the mean receptor content was lowest in myometrium (139±19 fmol/mg protein) with both leiomyomas (288±77 fmol/mg protein) and endometrium (372±96 fmol/mg protein) having significantly higher values. Endometrial GnRH binding varied from 596±42 in uteri that were GnRH-R positive in the endothelium alone to 231±49 when GnRH-R was present also in the other tissues. Endometrium negative for the GnRH-R had significantly higher levels of estrogen receptor than all the other uterine samples (266±25 vs 614±7.5 fmol/mg protein, respectively). Endometrial GnRH-R seem to be dependent on its presence and/or level in other uterine tissues. Further, when GnRH-R is absent in the endometrium this tissue expresses greatly increased levels of steroid receptors.
|Number of pages||5|
|Journal||International Journal of Oncology|
|Publication status||Published - 1997|
- Steroid receptors
ASJC Scopus subject areas
- Cancer Research