gp91phox-dependent expression of platelet CD40 ligand

P. Pignatelli, V. Sanguigni, L. Lenti, D. Ferro, A. Finocchi, P. Rossi, Francesco Violi

Research output: Contribution to journalArticle

Abstract

Background-CD40 ligand (CD40L) expression on platelets is mediated by agonists, but the underlying mechanism is still unclear. Methods and Results-CD40L expression was measured in platelets from healthy subjects both with and without the addition of antioxidants or a phospholipase A2 (PLA2) inhibitor and in platelets from 2 patients with an inherited deficiency of gp91phox. Immunoprecipitation analysis was also performed to determine whether normal platelets showed gp91phox expression. Unlike catalase and mannitol, superoxide dismutase inhibited agonist-induced platelet CD40L expression in healthy subjects. Immunoprecipitation analysis also showed that platelets from healthy subjects expressed gp91phox. In 2 male patients with inherited gp91phox deficiency, collagen-, thrombin-, and arachidonic acid-stimulated platelets showed an almost complete absence of superoxide anion (O2 -) and CD40L expression. Incubation of platelets from healthy subjects with a PLA2 inhibitor almost completely prevented agonist-induced O2 - and CD40L expression. Conclusions-These data provide the first evidence that platelet CD40L expression occurs via arachidonic acid-mediated gp91phox activation.

Original languageEnglish
Pages (from-to)1326-1329
Number of pages4
JournalCirculation
Volume110
Issue number10
DOIs
Publication statusPublished - Sep 7 2004

Keywords

  • CD40 ligand
  • NADPH oxidase
  • Oxidative stress
  • Platelets

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

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    Pignatelli, P., Sanguigni, V., Lenti, L., Ferro, D., Finocchi, A., Rossi, P., & Violi, F. (2004). gp91phox-dependent expression of platelet CD40 ligand. Circulation, 110(10), 1326-1329. https://doi.org/10.1161/01.CIR.0000134963.77201.55