TY - JOUR
T1 - GPR17 expressing NG2-Glia
T2 - Oligodendrocyte progenitors serving as a reserve pool after injury
AU - Viganò, Francesca
AU - Schneider, Sarah
AU - Cimino, Mauro
AU - Bonfanti, Elisabetta
AU - Gelosa, Paolo
AU - Sironi, Luigi
AU - Abbracchio, Maria P.
AU - Dimou, Leda
PY - 2016/2/1
Y1 - 2016/2/1
N2 - In the adult brain NG2-glia continuously generate mature, myelinating oligodendrocytes. To which extent the differentiation process is common to all NG2-glia and whether distinct pools are recruited for repair under physiological and pathological conditions still needs clarification. Here, we aimed at investigating the differentiation potential of adult NG2-glia that specifically express the G-protein coupled receptor 17 (GPR17), a membrane receptor that regulates the differentiation of these cells at postnatal stages. To this aim, we generated the first BAC transgenic GPR17-iCreERT2 mouse line for fate mapping studies. In these mice, under physiological conditions, GPR17+ cells -in contrast to GPR17- NG2-glia- did not differentiate within 3 months, a peculiarity that was overcome after cerebral damage induced by acute injury or ischemia. After these insults, GPR17+ NG2-glia rapidly reacted to the damage and underwent maturation, suggesting that they represent a 'reserve pool' of adult progenitors maintained for repair purposes.
AB - In the adult brain NG2-glia continuously generate mature, myelinating oligodendrocytes. To which extent the differentiation process is common to all NG2-glia and whether distinct pools are recruited for repair under physiological and pathological conditions still needs clarification. Here, we aimed at investigating the differentiation potential of adult NG2-glia that specifically express the G-protein coupled receptor 17 (GPR17), a membrane receptor that regulates the differentiation of these cells at postnatal stages. To this aim, we generated the first BAC transgenic GPR17-iCreERT2 mouse line for fate mapping studies. In these mice, under physiological conditions, GPR17+ cells -in contrast to GPR17- NG2-glia- did not differentiate within 3 months, a peculiarity that was overcome after cerebral damage induced by acute injury or ischemia. After these insults, GPR17+ NG2-glia rapidly reacted to the damage and underwent maturation, suggesting that they represent a 'reserve pool' of adult progenitors maintained for repair purposes.
KW - Differentiation
KW - Ischemia
KW - Oligodendrocyte progenitors
KW - Oligodendrogenesis
KW - Repair
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U2 - 10.1002/glia.22929
DO - 10.1002/glia.22929
M3 - Article
AN - SCOPUS:84953836427
VL - 64
SP - 287
EP - 299
JO - GLIA
JF - GLIA
SN - 0894-1491
IS - 2
ER -