GPR17 expressing NG2-Glia: Oligodendrocyte progenitors serving as a reserve pool after injury

Francesca Viganò, Sarah Schneider, Mauro Cimino, Elisabetta Bonfanti, Paolo Gelosa, Luigi Sironi, Maria P. Abbracchio, Leda Dimou

Research output: Contribution to journalArticle

Abstract

In the adult brain NG2-glia continuously generate mature, myelinating oligodendrocytes. To which extent the differentiation process is common to all NG2-glia and whether distinct pools are recruited for repair under physiological and pathological conditions still needs clarification. Here, we aimed at investigating the differentiation potential of adult NG2-glia that specifically express the G-protein coupled receptor 17 (GPR17), a membrane receptor that regulates the differentiation of these cells at postnatal stages. To this aim, we generated the first BAC transgenic GPR17-iCreERT2 mouse line for fate mapping studies. In these mice, under physiological conditions, GPR17+ cells -in contrast to GPR17- NG2-glia- did not differentiate within 3 months, a peculiarity that was overcome after cerebral damage induced by acute injury or ischemia. After these insults, GPR17+ NG2-glia rapidly reacted to the damage and underwent maturation, suggesting that they represent a 'reserve pool' of adult progenitors maintained for repair purposes.

Original languageEnglish
Pages (from-to)287-299
Number of pages13
JournalGLIA
Volume64
Issue number2
DOIs
Publication statusPublished - Feb 1 2016

    Fingerprint

Keywords

  • Differentiation
  • Ischemia
  • Oligodendrocyte progenitors
  • Oligodendrogenesis
  • Repair

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Neurology

Cite this

Viganò, F., Schneider, S., Cimino, M., Bonfanti, E., Gelosa, P., Sironi, L., Abbracchio, M. P., & Dimou, L. (2016). GPR17 expressing NG2-Glia: Oligodendrocyte progenitors serving as a reserve pool after injury. GLIA, 64(2), 287-299. https://doi.org/10.1002/glia.22929