Grading keratinocyte atypia in actinic keratosis: A correlation of reflectance confocal microscopy and histopathology

G. Pellacani, M. Ulrich, A. Casari, T. W. Prow, F. Cannillo, E. Benati, A. Losi, A. M. Cesinaro, C. Longo, G. Argenziano, H. P. Soyer

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Background Actinic Keratosis (AK) is the clinical manifestation of cutaneous dysplasia of epidermal keratinocytes, with progressive trend towards squamous cell carcinoma. Objective To evaluate the strength of the correlation between keratinocyte atypia, as detected by Reflectance Confocal Microscopy (RCM) and histopathology, and to develop a more objective atypia grading scale for RCM quantification, through a discrete ranking. Methods A total of 48 AKs and two control areas (photodamaged and non-photodamaged skin) were selected for this study. All these areas were documented by RCM and biopsied for histopathology. One representative image of the epidermis was selected for RCM and for histopathology and used for side-by-side comparison with purpose written software. The assessor chose which of two images displayed more keratinocyte atypia, and an ordered list from the image showing the least to the most keratinocyte atypia was generated. Three evaluations were obtained for RCM and two for histopathology. Results Good interobserver correlation was obtained for RCM and histopathology grading, with high concordance between RCM and histopathology grading. Conclusions Expert rater scan consistently distinguish different grades of cytological atypia. Non-invasive RCM data from in vivo imaging can be graded for keratinocyte atypia, comparable to histopathological grading.

Original languageEnglish
Pages (from-to)2216-2221
Number of pages6
JournalJournal of the European Academy of Dermatology and Venereology
Volume29
Issue number11
DOIs
Publication statusPublished - Nov 1 2015

Fingerprint

Actinic Keratosis
Keratinocytes
Confocal Microscopy
Skin
Epidermis
Squamous Cell Carcinoma
Software

ASJC Scopus subject areas

  • Dermatology
  • Infectious Diseases

Cite this

Grading keratinocyte atypia in actinic keratosis : A correlation of reflectance confocal microscopy and histopathology. / Pellacani, G.; Ulrich, M.; Casari, A.; Prow, T. W.; Cannillo, F.; Benati, E.; Losi, A.; Cesinaro, A. M.; Longo, C.; Argenziano, G.; Soyer, H. P.

In: Journal of the European Academy of Dermatology and Venereology, Vol. 29, No. 11, 01.11.2015, p. 2216-2221.

Research output: Contribution to journalArticle

Pellacani, G, Ulrich, M, Casari, A, Prow, TW, Cannillo, F, Benati, E, Losi, A, Cesinaro, AM, Longo, C, Argenziano, G & Soyer, HP 2015, 'Grading keratinocyte atypia in actinic keratosis: A correlation of reflectance confocal microscopy and histopathology', Journal of the European Academy of Dermatology and Venereology, vol. 29, no. 11, pp. 2216-2221. https://doi.org/10.1111/jdv.13215
Pellacani, G. ; Ulrich, M. ; Casari, A. ; Prow, T. W. ; Cannillo, F. ; Benati, E. ; Losi, A. ; Cesinaro, A. M. ; Longo, C. ; Argenziano, G. ; Soyer, H. P. / Grading keratinocyte atypia in actinic keratosis : A correlation of reflectance confocal microscopy and histopathology. In: Journal of the European Academy of Dermatology and Venereology. 2015 ; Vol. 29, No. 11. pp. 2216-2221.
@article{ca5abb9fc7c54aefbd10224835740c09,
title = "Grading keratinocyte atypia in actinic keratosis: A correlation of reflectance confocal microscopy and histopathology",
abstract = "Background Actinic Keratosis (AK) is the clinical manifestation of cutaneous dysplasia of epidermal keratinocytes, with progressive trend towards squamous cell carcinoma. Objective To evaluate the strength of the correlation between keratinocyte atypia, as detected by Reflectance Confocal Microscopy (RCM) and histopathology, and to develop a more objective atypia grading scale for RCM quantification, through a discrete ranking. Methods A total of 48 AKs and two control areas (photodamaged and non-photodamaged skin) were selected for this study. All these areas were documented by RCM and biopsied for histopathology. One representative image of the epidermis was selected for RCM and for histopathology and used for side-by-side comparison with purpose written software. The assessor chose which of two images displayed more keratinocyte atypia, and an ordered list from the image showing the least to the most keratinocyte atypia was generated. Three evaluations were obtained for RCM and two for histopathology. Results Good interobserver correlation was obtained for RCM and histopathology grading, with high concordance between RCM and histopathology grading. Conclusions Expert rater scan consistently distinguish different grades of cytological atypia. Non-invasive RCM data from in vivo imaging can be graded for keratinocyte atypia, comparable to histopathological grading.",
author = "G. Pellacani and M. Ulrich and A. Casari and Prow, {T. W.} and F. Cannillo and E. Benati and A. Losi and Cesinaro, {A. M.} and C. Longo and G. Argenziano and Soyer, {H. P.}",
year = "2015",
month = "11",
day = "1",
doi = "10.1111/jdv.13215",
language = "English",
volume = "29",
pages = "2216--2221",
journal = "Journal of the European Academy of Dermatology and Venereology",
issn = "0926-9959",
publisher = "wiley",
number = "11",

}

TY - JOUR

T1 - Grading keratinocyte atypia in actinic keratosis

T2 - A correlation of reflectance confocal microscopy and histopathology

AU - Pellacani, G.

AU - Ulrich, M.

AU - Casari, A.

AU - Prow, T. W.

AU - Cannillo, F.

AU - Benati, E.

AU - Losi, A.

AU - Cesinaro, A. M.

AU - Longo, C.

AU - Argenziano, G.

AU - Soyer, H. P.

PY - 2015/11/1

Y1 - 2015/11/1

N2 - Background Actinic Keratosis (AK) is the clinical manifestation of cutaneous dysplasia of epidermal keratinocytes, with progressive trend towards squamous cell carcinoma. Objective To evaluate the strength of the correlation between keratinocyte atypia, as detected by Reflectance Confocal Microscopy (RCM) and histopathology, and to develop a more objective atypia grading scale for RCM quantification, through a discrete ranking. Methods A total of 48 AKs and two control areas (photodamaged and non-photodamaged skin) were selected for this study. All these areas were documented by RCM and biopsied for histopathology. One representative image of the epidermis was selected for RCM and for histopathology and used for side-by-side comparison with purpose written software. The assessor chose which of two images displayed more keratinocyte atypia, and an ordered list from the image showing the least to the most keratinocyte atypia was generated. Three evaluations were obtained for RCM and two for histopathology. Results Good interobserver correlation was obtained for RCM and histopathology grading, with high concordance between RCM and histopathology grading. Conclusions Expert rater scan consistently distinguish different grades of cytological atypia. Non-invasive RCM data from in vivo imaging can be graded for keratinocyte atypia, comparable to histopathological grading.

AB - Background Actinic Keratosis (AK) is the clinical manifestation of cutaneous dysplasia of epidermal keratinocytes, with progressive trend towards squamous cell carcinoma. Objective To evaluate the strength of the correlation between keratinocyte atypia, as detected by Reflectance Confocal Microscopy (RCM) and histopathology, and to develop a more objective atypia grading scale for RCM quantification, through a discrete ranking. Methods A total of 48 AKs and two control areas (photodamaged and non-photodamaged skin) were selected for this study. All these areas were documented by RCM and biopsied for histopathology. One representative image of the epidermis was selected for RCM and for histopathology and used for side-by-side comparison with purpose written software. The assessor chose which of two images displayed more keratinocyte atypia, and an ordered list from the image showing the least to the most keratinocyte atypia was generated. Three evaluations were obtained for RCM and two for histopathology. Results Good interobserver correlation was obtained for RCM and histopathology grading, with high concordance between RCM and histopathology grading. Conclusions Expert rater scan consistently distinguish different grades of cytological atypia. Non-invasive RCM data from in vivo imaging can be graded for keratinocyte atypia, comparable to histopathological grading.

UR - http://www.scopus.com/inward/record.url?scp=84945451562&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84945451562&partnerID=8YFLogxK

U2 - 10.1111/jdv.13215

DO - 10.1111/jdv.13215

M3 - Article

C2 - 26274903

AN - SCOPUS:84945451562

VL - 29

SP - 2216

EP - 2221

JO - Journal of the European Academy of Dermatology and Venereology

JF - Journal of the European Academy of Dermatology and Venereology

SN - 0926-9959

IS - 11

ER -