Granulation tissue-derived mesenchymal stromal cells: A potential application for burn wound healing in pediatric patients

Gloria Pelizzo, Maria Antonietta Avanzini, Melissa Mantelli, Stefania Croce, Alice Maltese, Elettra Vestri, Annalisa De Silvestri, Elena Percivalle, Valeria Calcaterra

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: Multipotential cells are mobilized into peripheral blood in response to trauma, in particular in severe burns. These cells migrate to the site of injury in response to chemotactic signals to modulate inflammation, repair damaged tissue and facilitate tissue regeneration. We evaluated the possibility of isolating and in vitro expand mesenchymal stromal cells (MSCs) from granulation tissue (GT) during debridement of a burn wound, as a persective strategy to improve skin regeneration. Methods: GT obtained from a 12-month-old burn patient was in vitro cultured. Expanded MCSs were characterized for morphology, immunophenotype, differentiation capacity and proliferative growth. Antifibrotic features were also evaluated. Results: It was possible to isolate and in vitro expand cells from GT with the morphology, phenotype, proliferative and differentiation capacity typical of MSC, these cells were defined as GT-MSC. GT-MSCs exhibited antifibrotic features by releasing soluble factors, this activity was superior to that observed in BM-MSC. Conclusions: Successful isolation and expansion of MSCs from GT is reported. Considering their functional characteristics, GT-MSCs could be considered a good candidate adjuvant therapy to improve burn wound healing, particularly in pediatrics.

Original languageEnglish
Pages (from-to)53-58
Number of pages6
JournalJournal of Stem Cells and Regenerative Medicine
Volume14
Issue number1
Publication statusPublished - Apr 1 2018

Keywords

  • Burn
  • Children
  • Granulation tissue
  • Mesenchymal stromal cells
  • Wound regeneration

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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