Granulocyte-macrophage colony-stimulating factor and interleukin-3 cause basophil histamine release by a common pathway

Downregulation by sodium

A. Tedeschi, C. Salmaso, M. Di Donato, M. Lorini, A. Miadonna

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-3 (IL-3) are recognized as enhancers, but not as inducers, of histamine release from normal human basophils. However, when extracellular Na+ is removed IL-3 acquires the capacity to induce histamine release. The aim of this study was to evaluate whether GM-CSF can induce basophil histamine release using the same pathway of IL-3. Leucocyte suspensions from normal human subjects were stimulated with GM-CSF, IL-3 and anti-IgE, and histamine release was evaluated by an automated fluorometric method. In a physiological medium, GM-CSF (10 ng/ml) and IL-3 (10 ng/ml) did not provoke histamine release, in spite of an efficient response to anti-IgE (10 μg/ml). However, when extracellular Na+ was substituted iso-osmotically with N- methyl-D-glucamine+ or with choline+, GM-CSF and IL-3 were able to trigger histamine release from either mixed leucocyte suspensions or purified human basophils. The effect of GM-CSF on basophil histamine release was dose dependent, with optimal release at a dose of 1 ng/ml after incubation at 37°for 60-120 min. The kinetics of IL-3-induced histamine release were similar, whereas anti-IgE-induced histamine release was more rapid, being almost maximal after incubation for 30 min. A good correlation was found between GM- CSF-induced and IL-3-induced histamine release; furthermore, the combined effects of the two cytokines were less than additive, suggesting that they share the same pathways leading to histamine release. When extracellular Na+ concentration was increased from 0 to 140 mM, histamine release induced by GM-CSF, IL-3 and anti-IgE was reduced progressively. In contrast, histamine release induced by these stimuli was upregulated when the concentration of extracellular Ca2+ was increased. These results provide indirect evidence that GM-CSF and IL-3 can induce basophil histamine release by a common pathway that is downregulated by Na+.

Original languageEnglish
Pages (from-to)164-170
Number of pages7
JournalImmunology
Volume96
Issue number2
DOIs
Publication statusPublished - 1999

Fingerprint

Basophils
Histamine Release
Interleukin-3
Granulocyte-Macrophage Colony-Stimulating Factor
Down-Regulation
Sodium
Suspensions
Leukocytes
Choline
Interleukin-10

ASJC Scopus subject areas

  • Immunology

Cite this

Granulocyte-macrophage colony-stimulating factor and interleukin-3 cause basophil histamine release by a common pathway : Downregulation by sodium. / Tedeschi, A.; Salmaso, C.; Di Donato, M.; Lorini, M.; Miadonna, A.

In: Immunology, Vol. 96, No. 2, 1999, p. 164-170.

Research output: Contribution to journalArticle

Tedeschi, A. ; Salmaso, C. ; Di Donato, M. ; Lorini, M. ; Miadonna, A. / Granulocyte-macrophage colony-stimulating factor and interleukin-3 cause basophil histamine release by a common pathway : Downregulation by sodium. In: Immunology. 1999 ; Vol. 96, No. 2. pp. 164-170.
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abstract = "Granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-3 (IL-3) are recognized as enhancers, but not as inducers, of histamine release from normal human basophils. However, when extracellular Na+ is removed IL-3 acquires the capacity to induce histamine release. The aim of this study was to evaluate whether GM-CSF can induce basophil histamine release using the same pathway of IL-3. Leucocyte suspensions from normal human subjects were stimulated with GM-CSF, IL-3 and anti-IgE, and histamine release was evaluated by an automated fluorometric method. In a physiological medium, GM-CSF (10 ng/ml) and IL-3 (10 ng/ml) did not provoke histamine release, in spite of an efficient response to anti-IgE (10 μg/ml). However, when extracellular Na+ was substituted iso-osmotically with N- methyl-D-glucamine+ or with choline+, GM-CSF and IL-3 were able to trigger histamine release from either mixed leucocyte suspensions or purified human basophils. The effect of GM-CSF on basophil histamine release was dose dependent, with optimal release at a dose of 1 ng/ml after incubation at 37°for 60-120 min. The kinetics of IL-3-induced histamine release were similar, whereas anti-IgE-induced histamine release was more rapid, being almost maximal after incubation for 30 min. A good correlation was found between GM- CSF-induced and IL-3-induced histamine release; furthermore, the combined effects of the two cytokines were less than additive, suggesting that they share the same pathways leading to histamine release. When extracellular Na+ concentration was increased from 0 to 140 mM, histamine release induced by GM-CSF, IL-3 and anti-IgE was reduced progressively. In contrast, histamine release induced by these stimuli was upregulated when the concentration of extracellular Ca2+ was increased. These results provide indirect evidence that GM-CSF and IL-3 can induce basophil histamine release by a common pathway that is downregulated by Na+.",
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