GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR TO HARVEST CIRCULATING HAEMOPOIETIC STEM CELLS FOR AUTOTRANSPLANTATION

AlessandroM Gianni, Marco Bregni, AngelikaC Stern, Salvatore Siena, Corrado Tarella, Alessandro Pileri, Gianni Bonadonna

Research output: Contribution to journalArticle

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Abstract

Granulocyte-macrophage colony-stimulating factor (GM-CSF), given to accelerate recovery from cytopenia induced by high-dose (7 g/m2) cyclophosphamide, reproducibly brought about a dramatic increase (up to 1000-fold) in the number of peripheral blood granulocyte-macrophage colony-forming units (CFU-GM). These circulating progenitors were harvested by leucapheresis and reinfused, together with autologous bone marrow cells, in seven patients with cancer after total body irradiation and melphalan. Complete haemopoietic recovery occurred in all seven transplanted patients in a very short time: mean (SD) 9·1 (0·9) days (range 8-11) to achieve more than 0·5 x 109/l neutrophils, 9·9 (1·7) days (range 8-13) to over 1 x 109/l neutrophils, 10·7 (2·6) days (range 9-16) to over 0·5 x 1011/l platelets, and 13·6 (4·2) days (range 13-21) to over 1·0 x 1011/l platelets. A reduction in the severity of mucositis was also observed. The rapid haematological recovery made possible by this approach promises to increase the therapeutic index of high-dose chemoradiotherapy regimens and to widen their role as treatment for chemoradiosensitive tumours.

Original languageEnglish
Pages (from-to)580-585
Number of pages6
JournalLancet
Volume334
Issue number8663
DOIs
Publication statusPublished - Sep 9 1989

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Granulocyte-Macrophage Progenitor Cells
Autologous Transplantation
Granulocyte-Macrophage Colony-Stimulating Factor
Neutrophils
Stem Cells
Blood Platelets
Mucositis
Melphalan
Whole-Body Irradiation
Chemoradiotherapy
Bone Marrow Cells
Cyclophosphamide
Neoplasms
Therapeutics

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Gianni, A., Bregni, M., Stern, A., Siena, S., Tarella, C., Pileri, A., & Bonadonna, G. (1989). GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR TO HARVEST CIRCULATING HAEMOPOIETIC STEM CELLS FOR AUTOTRANSPLANTATION. Lancet, 334(8663), 580-585. https://doi.org/10.1016/S0140-6736(89)90711-3

GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR TO HARVEST CIRCULATING HAEMOPOIETIC STEM CELLS FOR AUTOTRANSPLANTATION. / Gianni, AlessandroM; Bregni, Marco; Stern, AngelikaC; Siena, Salvatore; Tarella, Corrado; Pileri, Alessandro; Bonadonna, Gianni.

In: Lancet, Vol. 334, No. 8663, 09.09.1989, p. 580-585.

Research output: Contribution to journalArticle

Gianni, A, Bregni, M, Stern, A, Siena, S, Tarella, C, Pileri, A & Bonadonna, G 1989, 'GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR TO HARVEST CIRCULATING HAEMOPOIETIC STEM CELLS FOR AUTOTRANSPLANTATION', Lancet, vol. 334, no. 8663, pp. 580-585. https://doi.org/10.1016/S0140-6736(89)90711-3
Gianni, AlessandroM ; Bregni, Marco ; Stern, AngelikaC ; Siena, Salvatore ; Tarella, Corrado ; Pileri, Alessandro ; Bonadonna, Gianni. / GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR TO HARVEST CIRCULATING HAEMOPOIETIC STEM CELLS FOR AUTOTRANSPLANTATION. In: Lancet. 1989 ; Vol. 334, No. 8663. pp. 580-585.
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