Grass Plants Bind, Retain, Uptake, and Transport Infectious Prions

Sandra Pritzkow, Rodrigo Morales, Fabio Moda, Uffaf Khan, Glenn C. Telling, Edward Hoover, Claudio Soto

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Prions are the protein-based infectious agents responsible for prion diseases. Environmental prion contamination has been implicated in disease transmission. Here, we analyzed the binding and retention of infectious prion protein (PrPSc) to plants. Small quantities of PrPSc contained in diluted brain homogenate or in excretory materials (urine and feces) can bind to wheat grass roots and leaves. Wild-type hamsters were efficiently infected by ingestion of prion-contaminated plants. The prion-plant interaction occurs with prions from diverse origins, including chronic wasting disease. Furthermore, leaves contaminated by spraying with a prion-containing preparation retained PrPSc for several weeks in the living plant. Finally, plants can uptake prions from contaminated soil and transport them to aerial parts of the plant (stem and leaves). These findings demonstrate that plants can efficiently bind infectious prions and act as carriers of infectivity, suggesting a possible role of environmental prion contamination in the horizontal transmission of the disease.

Original languageEnglish
Pages (from-to)1168-1175
Number of pages8
JournalCell Reports
Volume11
Issue number8
DOIs
Publication statusPublished - May 26 2015

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Prions
Poaceae
Chronic Wasting Disease
Plant Stems
Contamination
Prion Diseases
Plant Leaves
PrPSc Proteins
Feces
Cricetinae
Triticum
Spraying
Soil
Eating
Urine
Brain
Antennas
Soils

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Pritzkow, S., Morales, R., Moda, F., Khan, U., Telling, G. C., Hoover, E., & Soto, C. (2015). Grass Plants Bind, Retain, Uptake, and Transport Infectious Prions. Cell Reports, 11(8), 1168-1175. https://doi.org/10.1016/j.celrep.2015.04.036

Grass Plants Bind, Retain, Uptake, and Transport Infectious Prions. / Pritzkow, Sandra; Morales, Rodrigo; Moda, Fabio; Khan, Uffaf; Telling, Glenn C.; Hoover, Edward; Soto, Claudio.

In: Cell Reports, Vol. 11, No. 8, 26.05.2015, p. 1168-1175.

Research output: Contribution to journalArticle

Pritzkow, S, Morales, R, Moda, F, Khan, U, Telling, GC, Hoover, E & Soto, C 2015, 'Grass Plants Bind, Retain, Uptake, and Transport Infectious Prions', Cell Reports, vol. 11, no. 8, pp. 1168-1175. https://doi.org/10.1016/j.celrep.2015.04.036
Pritzkow S, Morales R, Moda F, Khan U, Telling GC, Hoover E et al. Grass Plants Bind, Retain, Uptake, and Transport Infectious Prions. Cell Reports. 2015 May 26;11(8):1168-1175. https://doi.org/10.1016/j.celrep.2015.04.036
Pritzkow, Sandra ; Morales, Rodrigo ; Moda, Fabio ; Khan, Uffaf ; Telling, Glenn C. ; Hoover, Edward ; Soto, Claudio. / Grass Plants Bind, Retain, Uptake, and Transport Infectious Prions. In: Cell Reports. 2015 ; Vol. 11, No. 8. pp. 1168-1175.
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