Graves' disease: Phenotypic and functional analysis at the clonal level of the T-cell repertoire in peripheral blood and in thyroid

M. Bagnasco, D. Venuti, I. Prigione, G. C. Torre, S. Ferrini, G. W. Canonica

Research output: Contribution to journalArticle

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Abstract

We have investigated at the clonal level the repertoire of intrathyroid and peripheral T lymphocytes in three patients with Graves' disease using a high efficiency cloning technique. Clonal efficiencies ranged from 10 to 31% for intrathyroid, and from 19 to 100% for peripheral T cells. In Graves' disease the phenotypic analysis showed similar percentages of CD3+ CD4+ CD8- and CD3+ CD4- CD8+ clones in thyroid infiltrates and peripheral blood. The functional evaluation showed similar or lower proportions of cytolytic clones in thyroid infiltrates with respect to peripheral blood. Furthermore, the proportions of intrathyroid and peripheral T-cell clones capable of releasing interleukin-2 and/or γ-interferon in response to mitogen stimulation were similar. Finally, 44% of intrathyroid clones were neither cytolytic nor able to release IL-2 and γ-interferon. These results are strikingly different from those obtained in Hashimoto's thyroiditis, where the large majority of intrathyroid T-cell clones are cytolytic and the proportions of clones able to release γ-IFN are remarkably increased in thyroid infiltrates when compared to those obtained from peripheral blood. Taken together, these data suggest a different role for T lymphocytes in the pathogenesis of the two major human autoimmune thyroid diseases.

Original languageEnglish
Pages (from-to)230-239
Number of pages10
JournalClinical Immunology and Immunopathology
Volume47
Issue number2
DOIs
Publication statusPublished - 1988

Fingerprint

Graves Disease
Thyroid Gland
Clone Cells
T-Lymphocytes
Interferons
Interleukin-2
Hashimoto Disease
Thyroid Diseases
Mitogens
Autoimmune Diseases
Organism Cloning

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pathology and Forensic Medicine

Cite this

Graves' disease : Phenotypic and functional analysis at the clonal level of the T-cell repertoire in peripheral blood and in thyroid. / Bagnasco, M.; Venuti, D.; Prigione, I.; Torre, G. C.; Ferrini, S.; Canonica, G. W.

In: Clinical Immunology and Immunopathology, Vol. 47, No. 2, 1988, p. 230-239.

Research output: Contribution to journalArticle

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