Green tea polyphenol extract attenuates ischemia/reperfusion injury of the gut

Carmelo Muià, Emanuela Mazzon, Rosanna Di Paola, Tiziana Genovese, Marta Menegazzi, Achille P. Caputi, Hisanori Suzuki, Salvatore Cuzzocrea

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Various studies have clearly demonstrated that green tea catechins possess potent antioxidative properties, and the preventive effects against various oxidative diseases have been reported. The aim of this study was to investigate the effect of green tea extract on the tissue injury caused by ischemia/reperfusion (I/R) of the gut. I/R injury of the intestine was caused by clamping both the superior mesenteric artery and the celiac trunk for 45 min followed by release of the clamp allowing reperfusion for 1 h or 4 h. This procedure results in splanchnic artery occlusion (SAO) shock. Rats subjected to SAO developed a significant fall in mean arterial blood pressure, and only 10% of the animals survived for the entire 4-h reperfusion period. Surviving animals were sacrificed for histological examination and biochemical studies. Rats subjected to SAO displayed a significant increase in tissue myeloperoxidase (MPO) activity and malondialdehyde (MDA) levels, significant increases in plasma tumor necrosis factor (TNF)-α levels and marked injury to the distal ileum. Increased immunoreactivity to nitrotyrosine was observed in the ileum of rats subjected to SAO. Staining of sections of the ileum obtained from SAO rats with anti-intercellular adhesion molecule (ICAM-1) antibody and with anti-P-selectin antibody resulted in diffuse staining. Administration of green tea extract (20 and 10 mg kg-1 i.v.) 15 min prior to the onset of gut reperfusion significantly reduced in a dose-dependent manner the fall in mean arterial blood pressure, the mortality rate, infiltration of the reperfused intestine with polymorphonuclear neutrophils (MPO activity), lipid peroxidation (MDA levels), production of TNF-α, and histological evidence of gut injury. Administration of green tea extract also markedly reduced nitrotyrosine formation and the up-regulation of ICAM-1 and P-selectin during reperfusion. In order to clarify that green tea extract might be useful in the therapy of I/R injury, we also investigated the effect of green tea extract (20 mg kg -1 i.v.) when administered 5 min after the onset of gut reperfusion. Similar to the pretreatment approach, the post-treatment also significantly reduced the gut injury induced by I/R. These results demonstrate that green tea extract significantly reduces I/R injury of the intestine.

Original languageEnglish
Pages (from-to)364-374
Number of pages11
JournalNaunyn-Schmiedeberg's Archives of Pharmacology
Volume371
Issue number5
DOIs
Publication statusPublished - Aug 2005

Fingerprint

Polyphenols
Tea
Reperfusion Injury
Viscera
Reperfusion
Arteries
Arterial Pressure
Intercellular Adhesion Molecule-1
Ileum
Intestines
P-Selectin
Malondialdehyde
Peroxidase
Wounds and Injuries
Tumor Necrosis Factor-alpha
Staining and Labeling
Tissue Extracts
Superior Mesenteric Artery
Catechin
Constriction

Keywords

  • Epigallocatechin-3- gallate
  • Green tea catechins
  • NO
  • O
  • Peroxynitrite (ONOO-)
  • Splanchnic artery occlusion shock

ASJC Scopus subject areas

  • Pharmacology

Cite this

Green tea polyphenol extract attenuates ischemia/reperfusion injury of the gut. / Muià, Carmelo; Mazzon, Emanuela; Di Paola, Rosanna; Genovese, Tiziana; Menegazzi, Marta; Caputi, Achille P.; Suzuki, Hisanori; Cuzzocrea, Salvatore.

In: Naunyn-Schmiedeberg's Archives of Pharmacology, Vol. 371, No. 5, 08.2005, p. 364-374.

Research output: Contribution to journalArticle

Muià, C, Mazzon, E, Di Paola, R, Genovese, T, Menegazzi, M, Caputi, AP, Suzuki, H & Cuzzocrea, S 2005, 'Green tea polyphenol extract attenuates ischemia/reperfusion injury of the gut', Naunyn-Schmiedeberg's Archives of Pharmacology, vol. 371, no. 5, pp. 364-374. https://doi.org/10.1007/s00210-005-1076-0
Muià, Carmelo ; Mazzon, Emanuela ; Di Paola, Rosanna ; Genovese, Tiziana ; Menegazzi, Marta ; Caputi, Achille P. ; Suzuki, Hisanori ; Cuzzocrea, Salvatore. / Green tea polyphenol extract attenuates ischemia/reperfusion injury of the gut. In: Naunyn-Schmiedeberg's Archives of Pharmacology. 2005 ; Vol. 371, No. 5. pp. 364-374.
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