Group IV phospholipase A2α controls the formation of inter-cisternal continuities involved in intra-golgi transport

Enrica San Pietro, Mariagrazia Capestrano, Elena V. Polishchuk, Alessio DiPentima, Alvar Trucco, Pasquale Zizza, Stefania Mariggiò, Teodoro Pulvirenti, Michele Sallese, Stefano Tete, Alexander A. Mironov, Christina C. Leslie, Daniela Corda, Alberto Luini, Roman S. Polishchuk

Research output: Contribution to journalArticle

Abstract

The organization of intra-Golgi trafficking and the nature of the transport intermediates involved (e.g., vesicles, tubules, or tubular continuities) remain incompletely understood. It was recently shown that successive cisternae in the Golgi stack are interconnected by membrane tubules that form during the arrival of transport carriers from the endoplasmic reticulum. Here, we examine the mechanisms of generation and the function of these tubules. In principle, tubule formation might depend on several protein- and/or lipid-based mechanisms. Among the latter, we have studied the phospholipase A2 (PLA 2)-mediated generation of wedge-shaped lysolipids, with the resulting local positive membrane curvature. We show that the arrival of cargo at the Golgi complex induces the recruitment of Group IVA Ca2+-dependent, cytosolic PLA2 (cPLA2α) onto the Golgi complex itself, and that this cPLA2α is required for the formation of the traffic-dependent intercisternal tubules and for intra-Golgi transport. In contrast, silencing of cPLA2α has no inhibitory effects on peri-Golgi vesicles. These findings identify cPLA2α as the first component of the machinery that is responsible for the formation of intercisternal tubular continuities and support a role for these continuities in transport through the Golgi complex.

Original languageEnglish
Article numbere1000194
JournalPLoS Biology
Volume7
Issue number9
DOIs
Publication statusPublished - 2009

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

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    San Pietro, E., Capestrano, M., Polishchuk, E. V., DiPentima, A., Trucco, A., Zizza, P., Mariggiò, S., Pulvirenti, T., Sallese, M., Tete, S., Mironov, A. A., Leslie, C. C., Corda, D., Luini, A., & Polishchuk, R. S. (2009). Group IV phospholipase A2α controls the formation of inter-cisternal continuities involved in intra-golgi transport. PLoS Biology, 7(9), [e1000194]. https://doi.org/10.1371/journal.pbio.1000194