Growth arrest-inducing genes are activated in Dbl-transformed mouse fibroblasts

Raffaella Melani, Fabio Sallustio, Paolo Fardin, Cristina Vanni, Marzia Ognibene, Catherine Ottaviano, Giovanni Melillo, Luigi Varesio, Alessandra Eva

Research output: Contribution to journalArticle

Abstract

The Dbl oncogene is a guanine nucleotide exchange factor for Rho GTPases and its activity has been linked to the regulation of gene transcription. Dbl oncogene expression in NIH3T3 cells leads to changes in morphological and proliferative properties of these cells, inducing a highly transformed phenotype. To gain insights into Dbl oncogene-induced transformation we compared gene expression profiles between Dbl oncogene-transformed and parental NIH3T3 cells by cDNA microarray. We found that Dbl oncogene expression is associated with gene expression modulation involving upregulation of 51 genes and downregulation of 49 genes. Five of the overexpressed genes identified are known to exert antiproliferative functions. Our observations suggest that the expression of Dbl oncogene in NIH3T3 may lead to the induction of genes associated with cell cycle arrest, possibly through the activation of stress-induced kinases.

Original languageEnglish
Pages (from-to)155-165
Number of pages11
JournalGene Expression
Volume13
Issue number3
DOIs
Publication statusPublished - 2006

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Keywords

  • Dbl oncogene
  • Gene expression
  • Microarray

ASJC Scopus subject areas

  • Genetics

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