Growth conditions influence DNA methylation in cultured cerebellar granule cells

L. Bertino, M. Castiglione Ruffini, A. Copani, V. Bruno, G. Raciti, A. Cambria, F. Nicoletti

Research output: Contribution to journalArticlepeer-review


Growth conditions influenced DNA methylation in cultured cerebellar granule cells, as indicated by immunocytochemical analysis with monoclonal antibodies raised against 5-methylcytidine. In cultures grown under suboptimal conditions, i.e. in medium containing 10 instead of 25 mM K+, a substantial reduction in both the number of immunopositive cells and the intensity of immunostaining occurred at 4 days in vitro (DIV), a time which preceded the appearance of the morphological features of apoptosis. These results suggest that a reduction in DNA methylation is one of the biochemical events associated with the 'condemned phase' of apoptosis, in which granule cells grown under suboptimal conditions become committed to death.

Original languageEnglish
Pages (from-to)38-43
Number of pages6
JournalDevelopmental Brain Research
Issue number1
Publication statusPublished - Aug 20 1996


  • 5-Methylcytidine
  • Apoptosis
  • Cerebellar granule cell
  • Monoclonal antibody
  • Potassium

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience


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