Growth-factor independence of a new differentiated hepatitis B virus DNA-negative human hepatoma cell line

Patrizia Bagnarelli, Giulia Devescovi, Aldo Manzin, Italo Bearzi, Patrizia Bonazzi, Guido Carloni, Massimo Clementi

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Abstract

The establishment of a new, differentiated, hepatitis B virus DNA-negative, human hepatoma cell line (named PLC/AN/2) is described. Neoplastic liver tissue was obtained during hepatectomy in an HBsAg-negative man. The established cell line is negative for α-fetoprotein and carcinoembryonic antigen; it has retained in vitro some of the differentiated functions of normal hepatocytes. Additionally, it presents a distinctive rearrangement (translocation) at the long arm of chromosome 4. The high degree of independence from serum growth factor requirements appears to be a major in vitro characteristic of PLC/AN/2 cells, making them a suitable model system for the more precise definition of the human hepatocellular carcinoma phenotype, including mechanisms of growth control.

Original languageEnglish
Pages (from-to)1024-1032
Number of pages9
JournalHepatology
Volume11
Issue number6
Publication statusPublished - Jun 1990

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ASJC Scopus subject areas

  • Hepatology

Cite this

Bagnarelli, P., Devescovi, G., Manzin, A., Bearzi, I., Bonazzi, P., Carloni, G., & Clementi, M. (1990). Growth-factor independence of a new differentiated hepatitis B virus DNA-negative human hepatoma cell line. Hepatology, 11(6), 1024-1032.