Abstract
The establishment of a new, differentiated, hepatitis B virus DNA-negative, human hepatoma cell line (named PLC/AN/2) is described. Neoplastic liver tissue was obtained during hepatectomy in an HBsAg-negative man. The established cell line is negative for α-fetoprotein and carcinoembryonic antigen; it has retained in vitro some of the differentiated functions of normal hepatocytes. Additionally, it presents a distinctive rearrangement (translocation) at the long arm of chromosome 4. The high degree of independence from serum growth factor requirements appears to be a major in vitro characteristic of PLC/AN/2 cells, making them a suitable model system for the more precise definition of the human hepatocellular carcinoma phenotype, including mechanisms of growth control.
| Original language | English |
|---|---|
| Pages (from-to) | 1024-1032 |
| Number of pages | 9 |
| Journal | Hepatology |
| Volume | 11 |
| Issue number | 6 |
| Publication status | Published - Jun 1990 |
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ASJC Scopus subject areas
- Hepatology
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Growth-factor independence of a new differentiated hepatitis B virus DNA-negative human hepatoma cell line. / Bagnarelli, Patrizia; Devescovi, Giulia; Manzin, Aldo; Bearzi, Italo; Bonazzi, Patrizia; Carloni, Guido; Clementi, Massimo.
In: Hepatology, Vol. 11, No. 6, 06.1990, p. 1024-1032.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Growth-factor independence of a new differentiated hepatitis B virus DNA-negative human hepatoma cell line
AU - Bagnarelli, Patrizia
AU - Devescovi, Giulia
AU - Manzin, Aldo
AU - Bearzi, Italo
AU - Bonazzi, Patrizia
AU - Carloni, Guido
AU - Clementi, Massimo
PY - 1990/6
Y1 - 1990/6
N2 - The establishment of a new, differentiated, hepatitis B virus DNA-negative, human hepatoma cell line (named PLC/AN/2) is described. Neoplastic liver tissue was obtained during hepatectomy in an HBsAg-negative man. The established cell line is negative for α-fetoprotein and carcinoembryonic antigen; it has retained in vitro some of the differentiated functions of normal hepatocytes. Additionally, it presents a distinctive rearrangement (translocation) at the long arm of chromosome 4. The high degree of independence from serum growth factor requirements appears to be a major in vitro characteristic of PLC/AN/2 cells, making them a suitable model system for the more precise definition of the human hepatocellular carcinoma phenotype, including mechanisms of growth control.
AB - The establishment of a new, differentiated, hepatitis B virus DNA-negative, human hepatoma cell line (named PLC/AN/2) is described. Neoplastic liver tissue was obtained during hepatectomy in an HBsAg-negative man. The established cell line is negative for α-fetoprotein and carcinoembryonic antigen; it has retained in vitro some of the differentiated functions of normal hepatocytes. Additionally, it presents a distinctive rearrangement (translocation) at the long arm of chromosome 4. The high degree of independence from serum growth factor requirements appears to be a major in vitro characteristic of PLC/AN/2 cells, making them a suitable model system for the more precise definition of the human hepatocellular carcinoma phenotype, including mechanisms of growth control.
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M3 - Article
VL - 11
SP - 1024
EP - 1032
JO - Hepatology
JF - Hepatology
SN - 0270-9139
IS - 6
ER -