Growth-factor independence of a new differentiated hepatitis B virus DNA-negative human hepatoma cell line

Patrizia Bagnarelli, Giulia Devescovi, Aldo Manzin, Italo Bearzi, Patrizia Bonazzi, Guido Carloni, Massimo Clementi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The establishment of a new, differentiated, hepatitis B virus DNA-negative, human hepatoma cell line (named PLC/AN/2) is described. Neoplastic liver tissue was obtained during hepatectomy in an HBsAg-negative man. The established cell line is negative for α-fetoprotein and carcinoembryonic antigen; it has retained in vitro some of the differentiated functions of normal hepatocytes. Additionally, it presents a distinctive rearrangement (translocation) at the long arm of chromosome 4. The high degree of independence from serum growth factor requirements appears to be a major in vitro characteristic of PLC/AN/2 cells, making them a suitable model system for the more precise definition of the human hepatocellular carcinoma phenotype, including mechanisms of growth control.

Original languageEnglish
Pages (from-to)1024-1032
Number of pages9
JournalHepatology
Volume11
Issue number6
Publication statusPublished - Jun 1990

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Hepatitis B virus
Hepatocellular Carcinoma
Intercellular Signaling Peptides and Proteins
Fetal Proteins
Cell Line
Chromosomes, Human, Pair 4
DNA
Carcinoembryonic Antigen
Hepatectomy
Hepatitis B Surface Antigens
Hepatocytes
Phenotype
Liver
Growth
Serum
In Vitro Techniques

ASJC Scopus subject areas

  • Hepatology

Cite this

Bagnarelli, P., Devescovi, G., Manzin, A., Bearzi, I., Bonazzi, P., Carloni, G., & Clementi, M. (1990). Growth-factor independence of a new differentiated hepatitis B virus DNA-negative human hepatoma cell line. Hepatology, 11(6), 1024-1032.

Growth-factor independence of a new differentiated hepatitis B virus DNA-negative human hepatoma cell line. / Bagnarelli, Patrizia; Devescovi, Giulia; Manzin, Aldo; Bearzi, Italo; Bonazzi, Patrizia; Carloni, Guido; Clementi, Massimo.

In: Hepatology, Vol. 11, No. 6, 06.1990, p. 1024-1032.

Research output: Contribution to journalArticle

Bagnarelli, P, Devescovi, G, Manzin, A, Bearzi, I, Bonazzi, P, Carloni, G & Clementi, M 1990, 'Growth-factor independence of a new differentiated hepatitis B virus DNA-negative human hepatoma cell line', Hepatology, vol. 11, no. 6, pp. 1024-1032.
Bagnarelli P, Devescovi G, Manzin A, Bearzi I, Bonazzi P, Carloni G et al. Growth-factor independence of a new differentiated hepatitis B virus DNA-negative human hepatoma cell line. Hepatology. 1990 Jun;11(6):1024-1032.
Bagnarelli, Patrizia ; Devescovi, Giulia ; Manzin, Aldo ; Bearzi, Italo ; Bonazzi, Patrizia ; Carloni, Guido ; Clementi, Massimo. / Growth-factor independence of a new differentiated hepatitis B virus DNA-negative human hepatoma cell line. In: Hepatology. 1990 ; Vol. 11, No. 6. pp. 1024-1032.
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