Growth factors contained in platelets enhance proliferation of human mesenchymal stem cells

E. Lucarelli, A. Beccheroni, A. M. Del Vento, M. Reggiani, L. Sangiorgi, L. Bonaccorsi, P. Picci, A. Cenacchi, C. Meotti, P. M. Fomasari, M. Mercuri, D. Donati

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Mesenchymal stem cells (MSC) contained in the bone marrow provide an invaluable source for autologous progenitors cells for bone reconstruction. Major limitations in their use are: the relative low number of MSC in the marrow cell population and the small volume of bone marrow available. There are emerging data demonstrating clinical applications of ex-vivo expanded cells, whose expansion is supported by several growth factors. We investigated whether growth factors (GFs) released by platelets stimulated MSC proliferation. MTT assays showed that the effect of GFs on MSC proliferation was dose dependent and was maximal at 10%. Also frozen GFs were able to stimulate proliferation. In conclusion we identified a method to promote ex-vivo expansion of MSC to be used in bone reconstruction.

Original languageEnglish
Title of host publicationProceedings of the IEEE-EMBS Special Topic Conference on Molecular, Cellular and Tissue Engineering, MCTE 2002
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages155-156
Number of pages2
ISBN (Print)0780375572, 9780780375574
DOIs
Publication statusPublished - 2002
EventIEEE-EMBS Special Topic Conference on Molecular, Cellular and Tissue Engineering, MCTE 2002 - Genoa, Italy
Duration: Jun 6 2002Jun 9 2002

Other

OtherIEEE-EMBS Special Topic Conference on Molecular, Cellular and Tissue Engineering, MCTE 2002
CountryItaly
CityGenoa
Period6/6/026/9/02

Keywords

  • Mesenchymalstem cells
  • Platelets
  • Proliferation

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering
  • Biotechnology

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  • Cite this

    Lucarelli, E., Beccheroni, A., Del Vento, A. M., Reggiani, M., Sangiorgi, L., Bonaccorsi, L., Picci, P., Cenacchi, A., Meotti, C., Fomasari, P. M., Mercuri, M., & Donati, D. (2002). Growth factors contained in platelets enhance proliferation of human mesenchymal stem cells. In Proceedings of the IEEE-EMBS Special Topic Conference on Molecular, Cellular and Tissue Engineering, MCTE 2002 (pp. 155-156). [1175052] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MCTE.2002.1175052