Abstract
Information obtained mainly from in vitro culture studies and genetic analysis of mouse mutants White spotting and Steel indicate a pivotal role of growth factors in the development of mouse primordial germ cells (PGCs). While stem cell factor (SCF) and TGFβ1 seem to have a role in PGC migration (as an adhesion factor and a chemoattractant, respectively), the former is certainly required for PGC survival in vitro and probably in vivo as well. Recent findings suggest that the mechanism by which SCF supports PGC survival is by preventing PGC apoptosis. A similar action appears to be exerted by leukemia inhibitory factor (LIF), a further growth factor influencing PGC growth in culture. PGC proliferation seems to be mainly induced by cAMP dependent mechanisms, but futther investigations are needed to clarify the interrelationships among the different molecular pathways activated by SCF, LIF, cAMP and other putative PGC growth factors (i.e. bFGF). Stimulation of long-term proliferation of PGCs, leading to derivation of ES-like cells (embryonal germ cells) obtained by using a combination of growth factors (bFGF, SCF and LIF), opens new intriguing perspectives for such studies and transgenic technology.
| Original language | English |
|---|---|
| Pages (from-to) | 135-143 |
| Number of pages | 9 |
| Journal | Progress in Growth Factor Research |
| Volume | 5 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1994 |
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Keywords
- cyclic AMP
- embryonal germ cells
- leukemia inhibitory factor
- Primordial germ cells
- stem cell factor
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Immunology
- Endocrinology, Diabetes and Metabolism
- Immunology and Allergy
- Agricultural and Biological Sciences(all)
- Medicine(all)
Cite this
Growth factors in mouse primordial germ cell migration and proliferation. / De Felici, Massimo; Pesce, Maurizio.
In: Progress in Growth Factor Research, Vol. 5, No. 2, 1994, p. 135-143.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Growth factors in mouse primordial germ cell migration and proliferation
AU - De Felici, Massimo
AU - Pesce, Maurizio
PY - 1994
Y1 - 1994
N2 - Information obtained mainly from in vitro culture studies and genetic analysis of mouse mutants White spotting and Steel indicate a pivotal role of growth factors in the development of mouse primordial germ cells (PGCs). While stem cell factor (SCF) and TGFβ1 seem to have a role in PGC migration (as an adhesion factor and a chemoattractant, respectively), the former is certainly required for PGC survival in vitro and probably in vivo as well. Recent findings suggest that the mechanism by which SCF supports PGC survival is by preventing PGC apoptosis. A similar action appears to be exerted by leukemia inhibitory factor (LIF), a further growth factor influencing PGC growth in culture. PGC proliferation seems to be mainly induced by cAMP dependent mechanisms, but futther investigations are needed to clarify the interrelationships among the different molecular pathways activated by SCF, LIF, cAMP and other putative PGC growth factors (i.e. bFGF). Stimulation of long-term proliferation of PGCs, leading to derivation of ES-like cells (embryonal germ cells) obtained by using a combination of growth factors (bFGF, SCF and LIF), opens new intriguing perspectives for such studies and transgenic technology.
AB - Information obtained mainly from in vitro culture studies and genetic analysis of mouse mutants White spotting and Steel indicate a pivotal role of growth factors in the development of mouse primordial germ cells (PGCs). While stem cell factor (SCF) and TGFβ1 seem to have a role in PGC migration (as an adhesion factor and a chemoattractant, respectively), the former is certainly required for PGC survival in vitro and probably in vivo as well. Recent findings suggest that the mechanism by which SCF supports PGC survival is by preventing PGC apoptosis. A similar action appears to be exerted by leukemia inhibitory factor (LIF), a further growth factor influencing PGC growth in culture. PGC proliferation seems to be mainly induced by cAMP dependent mechanisms, but futther investigations are needed to clarify the interrelationships among the different molecular pathways activated by SCF, LIF, cAMP and other putative PGC growth factors (i.e. bFGF). Stimulation of long-term proliferation of PGCs, leading to derivation of ES-like cells (embryonal germ cells) obtained by using a combination of growth factors (bFGF, SCF and LIF), opens new intriguing perspectives for such studies and transgenic technology.
KW - cyclic AMP
KW - embryonal germ cells
KW - leukemia inhibitory factor
KW - Primordial germ cells
KW - stem cell factor
UR - http://www.scopus.com/inward/record.url?scp=0028083786&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028083786&partnerID=8YFLogxK
U2 - 10.1016/0955-2235(94)90001-9
DO - 10.1016/0955-2235(94)90001-9
M3 - Article
C2 - 7919220
AN - SCOPUS:0028083786
VL - 5
SP - 135
EP - 143
JO - Progress in Growth Factor Research
JF - Progress in Growth Factor Research
SN - 0955-2235
IS - 2
ER -