Growth fraction determined with monoclonal Ki-67 and it's correlation with indices of tumor differentiation in stage I-II breast cancer

G. Gasparini, P. Bevilacqua, S. Dal Fior, G. A. Panizzoni, S. Meli, A. Visona, F. Pozza

Research output: Contribution to journalArticlepeer-review

Abstract

In 147 patients with stage I-II breast cancer we tested the monoclonal antibody Ki-67 to determine the proliferative rates, using an immunocytochemical assay with anti-Ki-67 (Dakopatts clone) and avidin-biotin-peroxidase complex in frozen sections. One hundred two of 147 (69%) tumor specimens were reactive with a nuclear stain. The proportion of nuclei stained ranged from 5% to 65%; the mean value was 20%; the median, 18%; and the standard deviation, 14.5%. The growth fraction was correlated with the following indices of tumor differentiation: pathologic grade, fibrosis, elastosis, tumor necrosis, lymphoid infiltrates, and, in 133 cases of this series, with estrogen and progesterone hormonal receptors (ER and PgR) determined by standard dextran-coated-charcoal (DCC) assay, and with monoclonal antibodies (ER-ICA and PgR-ICA, Abbott Laboratory clones). Our study revealed a significant positive correlation between nuclear Ki-67 staining rate and grade (p = 0.04) and with the presence of tumor necrosis (p = 0.025). No correlation was observed between growth fraction and fibrosis or elastosis. An inverse significant correlation was found between growth fraction and lymphoid infiltrates (p = 0.05) and ER expression both with DCC (p = 0.0005) and with ER-ICA (p = 0.005) methods. Regarding PgR expression, no correlation was found with growth fraction using DCC, while a significant inverse correlation was found with the PgR-ICA (p <0.01) method. In conclusion, a strong inverse relationship between high growth fraction and tumor differentiation indices was found.

Original languageEnglish
Pages (from-to)205-213
Number of pages9
JournalBreast Disease
Volume3
Issue number4
Publication statusPublished - 1990

ASJC Scopus subject areas

  • Surgery

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