Abstract
The growth of human hepatoma Hep 3B cells is potently inhibited by TGF-β1, (ID50 = 0.2 ng/ml, 8 pM). A mutant cell line was derived that was not inhibited in growth by TGF-β1 at 5 ng/ml (200 pM) and that lacked TGF-β receptor type II (TGF-βRII) gene. Transfection of the cloned cDNA for human TGF-βRII to this mutant cell line restored receptor expression as well as the inhibition in growth by TGF-β1. In both wild-type and mutant cells stably transfected with TGF-βRII cDNA, TGF-βRII coimmunoprecipitated with TGF-β receptor type I in the presence of ligand. These experiments provide direct evidence for the role of TGF-βRII in the inhibitory effect of TGF-β on growth and suggest that TGF-βRII acts by means of a heteromeric surface complex with TGF-β receptor type I.
Original language | English |
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Pages (from-to) | 5359-5363 |
Number of pages | 5 |
Journal | Proceedings of the National Academy of Sciences of the United States of America |
Volume | 90 |
Issue number | 11 |
Publication status | Published - Jun 1 1993 |
Keywords
- Human hepatoma
- Polyclonal antisera
- Transfection
ASJC Scopus subject areas
- General
- Genetics