The growth of human hepatoma Hep 3B cells is potently inhibited by TGF-β1, (ID50 = 0.2 ng/ml, 8 pM). A mutant cell line was derived that was not inhibited in growth by TGF-β1 at 5 ng/ml (200 pM) and that lacked TGF-β receptor type II (TGF-βRII) gene. Transfection of the cloned cDNA for human TGF-βRII to this mutant cell line restored receptor expression as well as the inhibition in growth by TGF-β1. In both wild-type and mutant cells stably transfected with TGF-βRII cDNA, TGF-βRII coimmunoprecipitated with TGF-β receptor type I in the presence of ligand. These experiments provide direct evidence for the role of TGF-βRII in the inhibitory effect of TGF-β on growth and suggest that TGF-βRII acts by means of a heteromeric surface complex with TGF-β receptor type I.
|Number of pages||5|
|Journal||Proceedings of the National Academy of Sciences of the United States of America|
|Publication status||Published - Jun 1 1993|
- Human hepatoma
- Polyclonal antisera
ASJC Scopus subject areas