Growth inhibition by transforming growth factor β (TGF-β) type I is restored in TGF-β-resistant hepatoma cells after expression of TGF-β receptor type II cDNA

Mitsuhiro Inagaki, Aristidis Moustakas, Herbert Y. Lin, Harvey F. Lodish, Brian I. Carr

Research output: Contribution to journalArticlepeer-review

Abstract

The growth of human hepatoma Hep 3B cells is potently inhibited by TGF-β1, (ID50 = 0.2 ng/ml, 8 pM). A mutant cell line was derived that was not inhibited in growth by TGF-β1 at 5 ng/ml (200 pM) and that lacked TGF-β receptor type II (TGF-βRII) gene. Transfection of the cloned cDNA for human TGF-βRII to this mutant cell line restored receptor expression as well as the inhibition in growth by TGF-β1. In both wild-type and mutant cells stably transfected with TGF-βRII cDNA, TGF-βRII coimmunoprecipitated with TGF-β receptor type I in the presence of ligand. These experiments provide direct evidence for the role of TGF-βRII in the inhibitory effect of TGF-β on growth and suggest that TGF-βRII acts by means of a heteromeric surface complex with TGF-β receptor type I.

Original languageEnglish
Pages (from-to)5359-5363
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number11
Publication statusPublished - Jun 1 1993

Keywords

  • Human hepatoma
  • Polyclonal antisera
  • Transfection

ASJC Scopus subject areas

  • General
  • Genetics

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