Growth pattern of the human promyelocytic leukaemia cell line HL60

P. Foa, A. T. Maiolo, L. Lombardi, H. Toivonen, T. Rytömaa, E. E. Polli

Research output: Contribution to journalArticle

Abstract

In order to characterize the growth pattern of the human promyelocytic leukaemia cell line HL60, its kinetic parameters were studied. The doubling time was calculated from serial cell counts, the duration of the various cell cycle phases from the analysis of the labelled mitoses curve, and quiescent population from continuous labelling experiments. Proliferation in culture was exponential up to a saturation density of about 3.0 x 106 cells/ml, with a doubling time of 34.0 hr. The cell cycle duration was 24.3 ± 4.1 hr (SD), and that of the cell cycle phases was: G1, 3.8 ± 2.2 hr; S, 15.1 ± 3 hr; and G2, 5.4 ± 1.2 hr. The growth fraction was 0.85, and cell loss was restricted to the quiescent cells. The HL60 cell line, with fully characterized kinetics, provides a useful tool for the in vitro study of substances which may affect human leukaemic myelopoietic proliferation.

Original languageEnglish
Pages (from-to)399-404
Number of pages6
JournalCell and Tissue Kinetics
Volume15
Issue number4
Publication statusPublished - 1982

Fingerprint

Cell Cycle
Leukemia
Cell Line
Growth
HL-60 Cells
G1 Phase
Mitosis
Cell Count
Population
In Vitro Techniques

ASJC Scopus subject areas

  • Cell Biology

Cite this

Foa, P., Maiolo, A. T., Lombardi, L., Toivonen, H., Rytömaa, T., & Polli, E. E. (1982). Growth pattern of the human promyelocytic leukaemia cell line HL60. Cell and Tissue Kinetics, 15(4), 399-404.

Growth pattern of the human promyelocytic leukaemia cell line HL60. / Foa, P.; Maiolo, A. T.; Lombardi, L.; Toivonen, H.; Rytömaa, T.; Polli, E. E.

In: Cell and Tissue Kinetics, Vol. 15, No. 4, 1982, p. 399-404.

Research output: Contribution to journalArticle

Foa, P, Maiolo, AT, Lombardi, L, Toivonen, H, Rytömaa, T & Polli, EE 1982, 'Growth pattern of the human promyelocytic leukaemia cell line HL60', Cell and Tissue Kinetics, vol. 15, no. 4, pp. 399-404.
Foa P, Maiolo AT, Lombardi L, Toivonen H, Rytömaa T, Polli EE. Growth pattern of the human promyelocytic leukaemia cell line HL60. Cell and Tissue Kinetics. 1982;15(4):399-404.
Foa, P. ; Maiolo, A. T. ; Lombardi, L. ; Toivonen, H. ; Rytömaa, T. ; Polli, E. E. / Growth pattern of the human promyelocytic leukaemia cell line HL60. In: Cell and Tissue Kinetics. 1982 ; Vol. 15, No. 4. pp. 399-404.
@article{b4f7bbf335cc46f2ae641a172e6ade0f,
title = "Growth pattern of the human promyelocytic leukaemia cell line HL60",
abstract = "In order to characterize the growth pattern of the human promyelocytic leukaemia cell line HL60, its kinetic parameters were studied. The doubling time was calculated from serial cell counts, the duration of the various cell cycle phases from the analysis of the labelled mitoses curve, and quiescent population from continuous labelling experiments. Proliferation in culture was exponential up to a saturation density of about 3.0 x 106 cells/ml, with a doubling time of 34.0 hr. The cell cycle duration was 24.3 ± 4.1 hr (SD), and that of the cell cycle phases was: G1, 3.8 ± 2.2 hr; S, 15.1 ± 3 hr; and G2, 5.4 ± 1.2 hr. The growth fraction was 0.85, and cell loss was restricted to the quiescent cells. The HL60 cell line, with fully characterized kinetics, provides a useful tool for the in vitro study of substances which may affect human leukaemic myelopoietic proliferation.",
author = "P. Foa and Maiolo, {A. T.} and L. Lombardi and H. Toivonen and T. Ryt{\"o}maa and Polli, {E. E.}",
year = "1982",
language = "English",
volume = "15",
pages = "399--404",
journal = "Cell Proliferation",
issn = "0960-7722",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Growth pattern of the human promyelocytic leukaemia cell line HL60

AU - Foa, P.

AU - Maiolo, A. T.

AU - Lombardi, L.

AU - Toivonen, H.

AU - Rytömaa, T.

AU - Polli, E. E.

PY - 1982

Y1 - 1982

N2 - In order to characterize the growth pattern of the human promyelocytic leukaemia cell line HL60, its kinetic parameters were studied. The doubling time was calculated from serial cell counts, the duration of the various cell cycle phases from the analysis of the labelled mitoses curve, and quiescent population from continuous labelling experiments. Proliferation in culture was exponential up to a saturation density of about 3.0 x 106 cells/ml, with a doubling time of 34.0 hr. The cell cycle duration was 24.3 ± 4.1 hr (SD), and that of the cell cycle phases was: G1, 3.8 ± 2.2 hr; S, 15.1 ± 3 hr; and G2, 5.4 ± 1.2 hr. The growth fraction was 0.85, and cell loss was restricted to the quiescent cells. The HL60 cell line, with fully characterized kinetics, provides a useful tool for the in vitro study of substances which may affect human leukaemic myelopoietic proliferation.

AB - In order to characterize the growth pattern of the human promyelocytic leukaemia cell line HL60, its kinetic parameters were studied. The doubling time was calculated from serial cell counts, the duration of the various cell cycle phases from the analysis of the labelled mitoses curve, and quiescent population from continuous labelling experiments. Proliferation in culture was exponential up to a saturation density of about 3.0 x 106 cells/ml, with a doubling time of 34.0 hr. The cell cycle duration was 24.3 ± 4.1 hr (SD), and that of the cell cycle phases was: G1, 3.8 ± 2.2 hr; S, 15.1 ± 3 hr; and G2, 5.4 ± 1.2 hr. The growth fraction was 0.85, and cell loss was restricted to the quiescent cells. The HL60 cell line, with fully characterized kinetics, provides a useful tool for the in vitro study of substances which may affect human leukaemic myelopoietic proliferation.

UR - http://www.scopus.com/inward/record.url?scp=0019964862&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019964862&partnerID=8YFLogxK

M3 - Article

C2 - 6955024

VL - 15

SP - 399

EP - 404

JO - Cell Proliferation

JF - Cell Proliferation

SN - 0960-7722

IS - 4

ER -