Growth-stimulating activity of interleukin 6 on human mammary epithelial cells transfected with the int-2 gene

Fulvio Basolo, Simonetta Calvo, Lisa Fiore, Pier Giulio Conaldi, Valeria Falcone, Antonio Toniolo

Research output: Contribution to journalArticle

Abstract

We have shown recently that normal human mammary epithelial cells do produce interleukin 6 (IL6), interleukin 8, and a nonsecreted form of tumor necrosis factor. Here we report that ductal infiltrating mammary carcinomas fail to express immunoreactive IL6. Since abnormalities of cytokine genes are a frequent event in cancer, we investigated the production of and the response to cytokines of mammary cells using a panel of oncogene-transformed cells derived from the spontaneously immortalized MCF-10A cell line. We found that only the parental line and the int-2-transformed cells responded to exogenous IL6 and/or were suppressed by IL6-neutralizing antibody. In contrast to highly transformed cells, these two lines, which were either nontransformed (MCF-10A) or weakly transformed (int-2), were found to express IL6 receptors. These data suggest that loss of IL6 pathways can be a marker of mammary cell transformation.

Original languageEnglish
Pages (from-to)2957-2960
Number of pages4
JournalCancer Research
Volume53
Issue number13
Publication statusPublished - Jul 1 1993

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Fingerprint Dive into the research topics of 'Growth-stimulating activity of interleukin 6 on human mammary epithelial cells transfected with the int-2 gene'. Together they form a unique fingerprint.

  • Cite this