GSTM1 and NAT2 polymorphisms and colon, lung and bladder cancer risk: A case-control study

Angela Zupa, Alessandro Sgambato, Gabriella Bianchino, Giuseppina Improta, Vitina Grieco, Giuseppe La Torre, Biagina Campisi, Antonio Traficante, Michele Aieta, Achille Cittadini

Research output: Contribution to journalArticle

Abstract

Background: Glutathione S-transferase M1 (GSTM1) and N-acetyltransferase-2 (NAT2) are phase II enzymes involved in the metabolism of xenobiotics and whose polymorphisms have been related to individual cancer risks. Patients and Methods: A case-control study was performed including 92 colon, 75 lung and 23 bladder cancer patients and 121 corresponding controls to verify the existence of an association between the main genetic polymorphisms of GSTM1 and NAT2 and the risk to develop cancer. Genomic DNA, isolated from 5 mL whole blood, was used to study GSTM1 and NAT2 polymorphisms using multiplex PCR and a PCR-RFLP technique, respectively. Results: GSTM1 homozygous null genotype was associated with an increased risk of colon cancer, especially in females and in younger patients. For NAT2 gene, the results suggest a role for the low acetylator phenotype in the development of colon and lung cancer, especially in females. In bladder cancer patients two rare NAT2 genotypes were found at a higher frequency compared with all the other groups. Conclusion: The results do not suggest a different distribution of GSTM1 and NAT2 polymorphisms in the studied population compared to those reported for other Caucasian populations and warrant further studies in order to evaluate their potential relationship with individual cancer risks.

Original languageEnglish
Pages (from-to)1709-1714
Number of pages6
JournalAnticancer Research
Volume29
Issue number5
Publication statusPublished - May 2009

Keywords

  • Cancer susceptibility
  • Gene polymorphisms
  • Southern Italian region
  • Tumorigenesis

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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    Zupa, A., Sgambato, A., Bianchino, G., Improta, G., Grieco, V., La Torre, G., Campisi, B., Traficante, A., Aieta, M., & Cittadini, A. (2009). GSTM1 and NAT2 polymorphisms and colon, lung and bladder cancer risk: A case-control study. Anticancer Research, 29(5), 1709-1714.