Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

Andrea Cossarizza; Hyun Dong Chang; Andreas Radbruch; Andreas Acs; Dieter Adam; Sabine Adam-Klages; William W. Agace; Nima Aghaeepour; Mübeccel Akdis; Matthieu Allez; Larissa Nogueira Almeida; Giorgia Alvisi; Graham Anderson; Immanuel Andrä; Francesco Annunziato; Achille Anselmo; Petra Bacher; Cosima T. Baldari; Sudipto Bari; Vincenzo Barnaba; Joana Barros-Martins; Luca Battistini; Wolfgang Bauer; Sabine Baumgart; Nicole Baumgarth; Dirk Baumjohann; Bianka Baying; Mary Bebawy; Burkhard Becher; Wolfgang Beisker; Vladimir Benes; Rudi Beyaert; Giovanna Borsellino; Andrea Cavani; Mario Clerici; Federico S. Colombo; Genny Del Zotto; Silvia Della Bella; Paolo Dellabona; Andrea Gori; Enrico Lugli; Alberto Mantovani; Ivana Marventano; Giuseppe Matarese; Lorenzo Moretta; Federica Piancone; Anna Rubartelli; Angela Santoni; Marina Saresella; Silvano Sozzani

doi:10.1002/eji.201970107

Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

Andrea Cossarizza, Hyun Dong Chang, Andreas Radbruch, Andreas Acs, Dieter Adam, Sabine Adam-Klages, William W. Agace, Nima Aghaeepour, Mübeccel Akdis, Matthieu Allez, Larissa Nogueira Almeida, Giorgia Alvisi, Graham Anderson, Immanuel Andrä, Francesco Annunziato, Achille Anselmo, Petra Bacher, Cosima T. Baldari, Sudipto Bari, Vincenzo BarnabaJoana Barros-Martins, Luca Battistini, Wolfgang Bauer, Sabine Baumgart, Nicole Baumgarth, Dirk Baumjohann, Bianka Baying, Mary Bebawy, Burkhard Becher, Wolfgang Beisker, Vladimir Benes, Rudi Beyaert, Giovanna Borsellino, Andrea Cavani, Mario Clerici, Federico S. Colombo, Genny Del Zotto, Silvia Della Bella, Paolo Dellabona, Andrea Gori, Enrico Lugli, Alberto Mantovani, Ivana Marventano, Giuseppe Matarese, Lorenzo Moretta, Federica Piancone, Anna Rubartelli, Angela Santoni, Marina Saresella, Silvano Sozzani

Research output: Contribution to journal › Article

Abstract

These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.

Original language	English
Pages (from-to)	1457-1973
Number of pages	517
Journal	European Journal of Immunology
Volume	49
Issue number	10
DOIs	https://doi.org/10.1002/eji.201970107
Publication status	Published - Oct 1 2019

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Cossarizza, A., Chang, H. D., Radbruch, A., Acs, A., Adam, D., Adam-Klages, S., Agace, W. W., Aghaeepour, N., Akdis, M., Allez, M., Almeida, L. N., Alvisi, G., Anderson, G., Andrä, I., Annunziato, F., Anselmo, A., Bacher, P., Baldari, C. T., Bari, S., ... Sozzani, S. (2019). Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition). European Journal of Immunology, 49(10), 1457-1973. https://doi.org/10.1002/eji.201970107

Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition). / Cossarizza, Andrea; Chang, Hyun Dong; Radbruch, Andreas; Acs, Andreas; Adam, Dieter; Adam-Klages, Sabine; Agace, William W.; Aghaeepour, Nima; Akdis, Mübeccel; Allez, Matthieu; Almeida, Larissa Nogueira; Alvisi, Giorgia; Anderson, Graham; Andrä, Immanuel; Annunziato, Francesco; Anselmo, Achille; Bacher, Petra; Baldari, Cosima T.; Bari, Sudipto; Barnaba, Vincenzo; Barros-Martins, Joana; Battistini, Luca; Bauer, Wolfgang; Baumgart, Sabine; Baumgarth, Nicole; Baumjohann, Dirk; Baying, Bianka; Bebawy, Mary; Becher, Burkhard; Beisker, Wolfgang; Benes, Vladimir; Beyaert, Rudi; Borsellino, Giovanna; Cavani, Andrea; Clerici, Mario ; Colombo, Federico S.; Del Zotto, Genny ; Della Bella, Silvia ; Dellabona, Paolo ; Gori, Andrea ; Lugli, Enrico ; Mantovani, Alberto ; Marventano, Ivana; Matarese, Giuseppe; Moretta, Lorenzo ; Piancone, Federica; Rubartelli, Anna; Santoni, Angela; Saresella, Marina ; Sozzani, Silvano.

In: European Journal of Immunology, Vol. 49, No. 10, 01.10.2019, p. 1457-1973.

Research output: Contribution to journal › Article

Cossarizza, A, Chang, HD, Radbruch, A, Acs, A, Adam, D, Adam-Klages, S, Agace, WW, Aghaeepour, N, Akdis, M, Allez, M, Almeida, LN, Alvisi, G, Anderson, G, Andrä, I, Annunziato, F, Anselmo, A, Bacher, P, Baldari, CT, Bari, S, Barnaba, V, Barros-Martins, J, Battistini, L, Bauer, W, Baumgart, S, Baumgarth, N, Baumjohann, D, Baying, B, Bebawy, M, Becher, B, Beisker, W, Benes, V, Beyaert, R, Borsellino, G, Cavani, A, Clerici, M , Colombo, FS , Del Zotto, G , Della Bella, S , Dellabona, P , Gori, A , Lugli, E , Mantovani, A , Marventano, I, Matarese, G, Moretta, L , Piancone, F, Rubartelli, A, Santoni, A, Saresella, M & Sozzani, S 2019, 'Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)', European Journal of Immunology, vol. 49, no. 10, pp. 1457-1973. https://doi.org/10.1002/eji.201970107

Cossarizza, Andrea ; Chang, Hyun Dong ; Radbruch, Andreas ; Acs, Andreas ; Adam, Dieter ; Adam-Klages, Sabine ; Agace, William W. ; Aghaeepour, Nima ; Akdis, Mübeccel ; Allez, Matthieu ; Almeida, Larissa Nogueira ; Alvisi, Giorgia ; Anderson, Graham ; Andrä, Immanuel ; Annunziato, Francesco ; Anselmo, Achille ; Bacher, Petra ; Baldari, Cosima T. ; Bari, Sudipto ; Barnaba, Vincenzo ; Barros-Martins, Joana ; Battistini, Luca ; Bauer, Wolfgang ; Baumgart, Sabine ; Baumgarth, Nicole ; Baumjohann, Dirk ; Baying, Bianka ; Bebawy, Mary ; Becher, Burkhard ; Beisker, Wolfgang ; Benes, Vladimir ; Beyaert, Rudi ; Borsellino, Giovanna ; Cavani, Andrea ; Clerici, Mario ; Colombo, Federico S. ; Del Zotto, Genny ; Della Bella, Silvia ; Dellabona, Paolo ; Gori, Andrea ; Lugli, Enrico ; Mantovani, Alberto ; Marventano, Ivana ; Matarese, Giuseppe ; Moretta, Lorenzo ; Piancone, Federica ; Rubartelli, Anna ; Santoni, Angela ; Saresella, Marina ; Sozzani, Silvano. / Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition). In: European Journal of Immunology. 2019 ; Vol. 49, No. 10. pp. 1457-1973.

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abstract = "These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.",

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