TY - JOUR
T1 - Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
AU - Cossarizza, Andrea
AU - Chang, Hyun Dong
AU - Radbruch, Andreas
AU - Acs, Andreas
AU - Adam, Dieter
AU - Adam-Klages, Sabine
AU - Agace, William W.
AU - Aghaeepour, Nima
AU - Akdis, Mübeccel
AU - Allez, Matthieu
AU - Almeida, Larissa Nogueira
AU - Alvisi, Giorgia
AU - Anderson, Graham
AU - Andrä, Immanuel
AU - Annunziato, Francesco
AU - Anselmo, Achille
AU - Bacher, Petra
AU - Baldari, Cosima T.
AU - Bari, Sudipto
AU - Barnaba, Vincenzo
AU - Barros-Martins, Joana
AU - Battistini, Luca
AU - Bauer, Wolfgang
AU - Baumgart, Sabine
AU - Baumgarth, Nicole
AU - Baumjohann, Dirk
AU - Baying, Bianka
AU - Bebawy, Mary
AU - Becher, Burkhard
AU - Beisker, Wolfgang
AU - Benes, Vladimir
AU - Beyaert, Rudi
AU - Borsellino, Giovanna
AU - Cavani, Andrea
AU - Clerici, Mario
AU - Colombo, Federico S.
AU - Del Zotto, Genny
AU - Della Bella, Silvia
AU - Dellabona, Paolo
AU - Gori, Andrea
AU - Lugli, Enrico
AU - Mantovani, Alberto
AU - Marventano, Ivana
AU - Matarese, Giuseppe
AU - Moretta, Lorenzo
AU - Piancone, Federica
AU - Rubartelli, Anna
AU - Santoni, Angela
AU - Saresella, Marina
AU - Sozzani, Silvano
PY - 2019/10/1
Y1 - 2019/10/1
N2 - These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.
AB - These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.
U2 - 10.1002/eji.201970107
DO - 10.1002/eji.201970107
M3 - Article
VL - 49
SP - 1457
EP - 1973
JO - European Journal of Immunology
JF - European Journal of Immunology
SN - 0014-2980
IS - 10
ER -