Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition): European Journal of Immunology

A Cossarizza; HD Chang; A Radbruch; A Acs; D Adam; S Adam-Klages; WW Agace; N Aghaeepour; M Akdis; M Allez; LN Almeida; G Alvisi; G Anderson; I Andrä; F Annunziato; A Anselmo; P Bacher; CT Baldari; S Bari; V Barnaba; J Barros-Martins; L Battistini; W Bauer; S Baumgart; N Baumgarth; D Baumjohann; B Baying; M Bebawy; B Becher; W Beisker; V Benes; R Beyaert; A Blanco; DA Boardman; C Bogdan; JG Borger; G Borsellino; PE Boulais; JA Bradford; D Brenner; RR Brinkman; AES Brooks; DH Busch; M Büscher; TP Bushnell; F Calzetti; G Cameron; I Cammarata; X Cao; SL Cardell; S Casola; MA Cassatella; A Cavani; A Celada; L Chatenoud; PK Chattopadhyay; S Chow; E Christakou; L Čičin-Šain; M Clerici; FS Colombo; L Cook; A Cooke; AM Cooper; AJ Corbett; A Cosma; L Cosmi; PG Coulie; A Cumano; L Cvetkovic; VD Dang; C Dang-Heine; MS Davey; D Davies; S De Biasi; G Del Zotto; GV Dela Cruz; M Delacher; S Della Bella; P Dellabona; G Deniz; M Dessing; JP Di Santo; A Diefenbach; F Dieli; A Dolf; T Dörner; RJ Dress; D Dudziak; M Dustin; CA Dutertre; F Ebner; SBG Eckle; M Edinger; P Eede; GRA Ehrhardt; M Eich; P Engel; B Engelhardt; A Erdei

doi:10.1002/eji.201970107

Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition): European Journal of Immunology

A Cossarizza, HD Chang, A Radbruch, A Acs, D Adam, S Adam-Klages, WW Agace, N Aghaeepour, M Akdis, M Allez, LN Almeida, G Alvisi, G Anderson, I Andrä, F Annunziato, A Anselmo, P Bacher, CT Baldari, S Bari, V BarnabaJ Barros-Martins, L Battistini, W Bauer, S Baumgart, N Baumgarth, D Baumjohann, B Baying, M Bebawy, B Becher, W Beisker, V Benes, R Beyaert, A Blanco, DA Boardman, C Bogdan, JG Borger, G Borsellino, PE Boulais, JA Bradford, D Brenner, RR Brinkman, AES Brooks, DH Busch, M Büscher, TP Bushnell, F Calzetti, G Cameron, I Cammarata, X Cao, SL Cardell, S Casola, MA Cassatella, A Cavani, A Celada, L Chatenoud, PK Chattopadhyay, S Chow, E Christakou, L Čičin-Šain, M Clerici, FS Colombo, L Cook, A Cooke, AM Cooper, AJ Corbett, A Cosma, L Cosmi, PG Coulie, A Cumano, L Cvetkovic, VD Dang, C Dang-Heine, MS Davey, D Davies, S De Biasi, G Del Zotto, GV Dela Cruz, M Delacher, S Della Bella, P Dellabona, G Deniz, M Dessing, JP Di Santo, A Diefenbach, F Dieli, A Dolf, T Dörner, RJ Dress, D Dudziak, M Dustin, CA Dutertre, F Ebner, SBG Eckle, M Edinger, P Eede, GRA Ehrhardt, M Eich, P Engel, B Engelhardt, A Erdei

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Abstract

These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion. © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Original language	English
Pages (from-to)	1457-1973
Number of pages	517
Journal	European Journal of Immunology
Volume	49
Issue number	10
DOIs	https://doi.org/10.1002/eji.201970107
Publication status	Published - 2019

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Cossarizza, A., Chang, HD., Radbruch, A., Acs, A., Adam, D., Adam-Klages, S., Agace, WW., Aghaeepour, N., Akdis, M., Allez, M., Almeida, LN., Alvisi, G., Anderson, G., Andrä, I., Annunziato, F., Anselmo, A., Bacher, P., Baldari, CT., Bari, S., ... Erdei, A. (2019). Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition): European Journal of Immunology. European Journal of Immunology, 49(10), 1457-1973. https://doi.org/10.1002/eji.201970107

Cossarizza, A, Chang, HD, Radbruch, A, Acs, A, Adam, D, Adam-Klages, S, Agace, WW, Aghaeepour, N, Akdis, M, Allez, M, Almeida, LN, Alvisi, G, Anderson, G, Andrä, I, Annunziato, F, Anselmo, A, Bacher, P, Baldari, CT, Bari, S, Barnaba, V, Barros-Martins, J, Battistini, L, Bauer, W, Baumgart, S, Baumgarth, N, Baumjohann, D, Baying, B, Bebawy, M, Becher, B, Beisker, W, Benes, V, Beyaert, R, Blanco, A, Boardman, DA, Bogdan, C, Borger, JG, Borsellino, G, Boulais, PE, Bradford, JA, Brenner, D, Brinkman, RR, Brooks, AES, Busch, DH, Büscher, M, Bushnell, TP, Calzetti, F, Cameron, G, Cammarata, I, Cao, X, Cardell, SL, Casola, S, Cassatella, MA, Cavani, A, Celada, A, Chatenoud, L, Chattopadhyay, PK, Chow, S, Christakou, E, Čičin-Šain, L, Clerici, M, Colombo, FS, Cook, L, Cooke, A, Cooper, AM, Corbett, AJ, Cosma, A, Cosmi, L, Coulie, PG, Cumano, A, Cvetkovic, L, Dang, VD, Dang-Heine, C, Davey, MS, Davies, D, De Biasi, S, Del Zotto, G, Dela Cruz, GV, Delacher, M, Della Bella, S, Dellabona, P, Deniz, G, Dessing, M, Di Santo, JP, Diefenbach, A, Dieli, F, Dolf, A, Dörner, T, Dress, RJ, Dudziak, D, Dustin, M, Dutertre, CA, Ebner, F, Eckle, SBG, Edinger, M, Eede, P, Ehrhardt, GRA, Eich, M, Engel, P, Engelhardt, B & Erdei, A 2019, 'Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition): European Journal of Immunology', European Journal of Immunology, vol. 49, no. 10, pp. 1457-1973. https://doi.org/10.1002/eji.201970107

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title = "Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition): European Journal of Immunology",

abstract = "These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion. {\textcopyright} 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim",

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year = "2019",

doi = "10.1002/eji.201970107",

language = "English",

volume = "49",

pages = "1457--1973",

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T1 - Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

T2 - European Journal of Immunology

AU - Cossarizza, A

AU - Chang, HD

AU - Radbruch, A

AU - Acs, A

AU - Adam, D

AU - Adam-Klages, S

AU - Agace, WW

AU - Aghaeepour, N

AU - Akdis, M

AU - Allez, M

AU - Almeida, LN

AU - Alvisi, G

AU - Anderson, G

AU - Andrä, I

AU - Annunziato, F

AU - Anselmo, A

AU - Bacher, P

AU - Baldari, CT

AU - Bari, S

AU - Barnaba, V

AU - Barros-Martins, J

AU - Battistini, L

AU - Bauer, W

AU - Baumgart, S

AU - Baumgarth, N

AU - Baumjohann, D

AU - Baying, B

AU - Bebawy, M

AU - Becher, B

AU - Beisker, W

AU - Benes, V

AU - Beyaert, R

AU - Blanco, A

AU - Boardman, DA

AU - Bogdan, C

AU - Borger, JG

AU - Borsellino, G

AU - Boulais, PE

AU - Bradford, JA

AU - Brenner, D

AU - Brinkman, RR

AU - Brooks, AES

AU - Busch, DH

AU - Büscher, M

AU - Bushnell, TP

AU - Calzetti, F

AU - Cameron, G

AU - Cammarata, I

AU - Cao, X

AU - Cardell, SL

AU - Casola, S

AU - Cassatella, MA

AU - Cavani, A

AU - Celada, A

AU - Chatenoud, L

AU - Chattopadhyay, PK

AU - Chow, S

AU - Christakou, E

AU - Čičin-Šain, L

AU - Clerici, M

AU - Colombo, FS

AU - Cook, L

AU - Cooke, A

AU - Cooper, AM

AU - Corbett, AJ

AU - Cosma, A

AU - Cosmi, L

AU - Coulie, PG

AU - Cumano, A

AU - Cvetkovic, L

AU - Dang, VD

AU - Dang-Heine, C

AU - Davey, MS

AU - Davies, D

AU - De Biasi, S

AU - Del Zotto, G

AU - Dela Cruz, GV

AU - Delacher, M

AU - Della Bella, S

AU - Dellabona, P

AU - Deniz, G

AU - Dessing, M

AU - Di Santo, JP

AU - Diefenbach, A

AU - Dieli, F

AU - Dolf, A

AU - Dörner, T

AU - Dress, RJ

AU - Dudziak, D

AU - Dustin, M

AU - Dutertre, CA

AU - Ebner, F

AU - Eckle, SBG

AU - Edinger, M

AU - Eede, P

AU - Ehrhardt, GRA

AU - Eich, M

AU - Engel, P

AU - Engelhardt, B

AU - Erdei, A

PY - 2019

Y1 - 2019

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AB - These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion. © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

U2 - 10.1002/eji.201970107

DO - 10.1002/eji.201970107

M3 - Article

VL - 49

SP - 1457

EP - 1973

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 10

ER -

Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition): European Journal of Immunology

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