Guidelines for the use of flow cytometry and cell sorting in immunological studies

Andrea Cossarizza; Hyun-Dong Chang; Andreas Radbruch; Mübeccel Akdis; Immanuel Andrä; Francesco Annunziato; Petra Bacher; Vincenzo Barnaba; Luca Battistini; Wolfgang M Bauer; Sabine Baumgart; Burkhard Becher; Wolfgang Beisker; Claudia Berek; Alfonso Blanco; Giovanna Borsellino; Philip E Boulais; Ryan R Brinkman; Martin Büscher; Dirk H Busch; Timothy P Bushnell; Xuetao Cao; Andrea Cavani; Pratip K Chattopadhyay; Qingyu Cheng; Sue Chow; Mario Clerici; Anne Cooke; Antonio Cosma; Lorenzo Cosmi; Ana Cumano; Van Duc Dang; Derek Davies; Sara De Biasi; Genny Del Zotto; Silvia Della Bella; Paolo Dellabona; Günnur Deniz; Mark Dessing; Andreas Diefenbach; James Di Santo; Francesco Dieli; Andreas Dolf; Vera S Donnenberg; Thomas Dörner; Götz R A Ehrhardt; Enrico Lugli; Giuseppe Matarese; Lorenzo Moretta; Anna Rubartelli

doi:10.1002/eji.201646632

Guidelines for the use of flow cytometry and cell sorting in immunological studies

Andrea Cossarizza, Hyun-Dong Chang, Andreas Radbruch, Mübeccel Akdis, Immanuel Andrä, Francesco Annunziato, Petra Bacher, Vincenzo Barnaba, Luca Battistini, Wolfgang M Bauer, Sabine Baumgart, Burkhard Becher, Wolfgang Beisker, Claudia Berek, Alfonso Blanco, Giovanna Borsellino, Philip E Boulais, Ryan R Brinkman, Martin Büscher, Dirk H BuschTimothy P Bushnell, Xuetao Cao, Andrea Cavani, Pratip K Chattopadhyay, Qingyu Cheng, Sue Chow, Mario Clerici, Anne Cooke, Antonio Cosma, Lorenzo Cosmi, Ana Cumano, Van Duc Dang, Derek Davies, Sara De Biasi, Genny Del Zotto, Silvia Della Bella, Paolo Dellabona, Günnur Deniz, Mark Dessing, Andreas Diefenbach, James Di Santo, Francesco Dieli, Andreas Dolf, Vera S Donnenberg, Thomas Dörner, Götz R A Ehrhardt, Enrico Lugli, Giuseppe Matarese, Lorenzo Moretta, Anna Rubartelli

Research output: Contribution to journal › Article

Original language	English
Pages (from-to)	1584-1797
Number of pages	214
Journal	European Journal of Immunology
Volume	47
Issue number	10
DOIs	https://doi.org/10.1002/eji.201646632
Publication status	Published - Oct 2017

Keywords

Animals
Cell Proliferation
Cell Separation
DNA
False Positive Reactions
Flow Cytometry
Guidelines as Topic
Humans
Immunologic Techniques
Immunophenotyping
Quality Control
RNA
Research Design
Software
T-Lymphocytes
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Cite this

Cossarizza, A., Chang, H-D., Radbruch, A., Akdis, M., Andrä, I., Annunziato, F., Bacher, P., Barnaba, V., Battistini, L., Bauer, W. M., Baumgart, S., Becher, B., Beisker, W., Berek, C., Blanco, A., Borsellino, G., Boulais, P. E., Brinkman, R. R., Büscher, M., ... Rubartelli, A. (2017). Guidelines for the use of flow cytometry and cell sorting in immunological studies. European Journal of Immunology, 47(10), 1584-1797. https://doi.org/10.1002/eji.201646632

Guidelines for the use of flow cytometry and cell sorting in immunological studies. / Cossarizza, Andrea; Chang, Hyun-Dong; Radbruch, Andreas; Akdis, Mübeccel; Andrä, Immanuel; Annunziato, Francesco; Bacher, Petra; Barnaba, Vincenzo; Battistini, Luca; Bauer, Wolfgang M; Baumgart, Sabine; Becher, Burkhard; Beisker, Wolfgang; Berek, Claudia; Blanco, Alfonso; Borsellino, Giovanna; Boulais, Philip E; Brinkman, Ryan R; Büscher, Martin; Busch, Dirk H; Bushnell, Timothy P; Cao, Xuetao; Cavani, Andrea; Chattopadhyay, Pratip K; Cheng, Qingyu; Chow, Sue; Clerici, Mario; Cooke, Anne; Cosma, Antonio; Cosmi, Lorenzo; Cumano, Ana; Dang, Van Duc; Davies, Derek; De Biasi, Sara; Del Zotto, Genny ; Della Bella, Silvia ; Dellabona, Paolo; Deniz, Günnur; Dessing, Mark; Diefenbach, Andreas; Di Santo, James; Dieli, Francesco; Dolf, Andreas; Donnenberg, Vera S; Dörner, Thomas; Ehrhardt, Götz R A; Lugli, Enrico; Matarese, Giuseppe; Moretta, Lorenzo; Rubartelli, Anna.

In: European Journal of Immunology, Vol. 47, No. 10, 10.2017, p. 1584-1797.

Research output: Contribution to journal › Article

Cossarizza, A, Chang, H-D, Radbruch, A, Akdis, M, Andrä, I, Annunziato, F, Bacher, P, Barnaba, V, Battistini, L, Bauer, WM, Baumgart, S, Becher, B, Beisker, W, Berek, C, Blanco, A, Borsellino, G, Boulais, PE, Brinkman, RR, Büscher, M, Busch, DH, Bushnell, TP, Cao, X, Cavani, A, Chattopadhyay, PK, Cheng, Q, Chow, S, Clerici, M, Cooke, A, Cosma, A, Cosmi, L, Cumano, A, Dang, VD, Davies, D, De Biasi, S, Del Zotto, G , Della Bella, S , Dellabona, P, Deniz, G, Dessing, M, Diefenbach, A, Di Santo, J, Dieli, F, Dolf, A, Donnenberg, VS, Dörner, T, Ehrhardt, GRA, Lugli, E, Matarese, G, Moretta, L & Rubartelli, A 2017, 'Guidelines for the use of flow cytometry and cell sorting in immunological studies', European Journal of Immunology, vol. 47, no. 10, pp. 1584-1797. https://doi.org/10.1002/eji.201646632

Cossarizza, Andrea ; Chang, Hyun-Dong ; Radbruch, Andreas ; Akdis, Mübeccel ; Andrä, Immanuel ; Annunziato, Francesco ; Bacher, Petra ; Barnaba, Vincenzo ; Battistini, Luca ; Bauer, Wolfgang M ; Baumgart, Sabine ; Becher, Burkhard ; Beisker, Wolfgang ; Berek, Claudia ; Blanco, Alfonso ; Borsellino, Giovanna ; Boulais, Philip E ; Brinkman, Ryan R ; Büscher, Martin ; Busch, Dirk H ; Bushnell, Timothy P ; Cao, Xuetao ; Cavani, Andrea ; Chattopadhyay, Pratip K ; Cheng, Qingyu ; Chow, Sue ; Clerici, Mario ; Cooke, Anne ; Cosma, Antonio ; Cosmi, Lorenzo ; Cumano, Ana ; Dang, Van Duc ; Davies, Derek ; De Biasi, Sara ; Del Zotto, Genny ; Della Bella, Silvia ; Dellabona, Paolo ; Deniz, Günnur ; Dessing, Mark ; Diefenbach, Andreas ; Di Santo, James ; Dieli, Francesco ; Dolf, Andreas ; Donnenberg, Vera S ; Dörner, Thomas ; Ehrhardt, Götz R A ; Lugli, Enrico ; Matarese, Giuseppe ; Moretta, Lorenzo ; Rubartelli, Anna. / Guidelines for the use of flow cytometry and cell sorting in immunological studies. In: European Journal of Immunology. 2017 ; Vol. 47, No. 10. pp. 1584-1797.

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AU - Chang, Hyun-Dong

AU - Radbruch, Andreas

AU - Akdis, Mübeccel

AU - Andrä, Immanuel

AU - Annunziato, Francesco

AU - Bacher, Petra

AU - Barnaba, Vincenzo

AU - Battistini, Luca

AU - Bauer, Wolfgang M

AU - Baumgart, Sabine

AU - Becher, Burkhard

AU - Beisker, Wolfgang

AU - Berek, Claudia

AU - Blanco, Alfonso

AU - Borsellino, Giovanna

AU - Boulais, Philip E

AU - Brinkman, Ryan R

AU - Büscher, Martin

AU - Busch, Dirk H

AU - Bushnell, Timothy P

AU - Cao, Xuetao

AU - Cavani, Andrea

AU - Chattopadhyay, Pratip K

AU - Cheng, Qingyu

AU - Chow, Sue

AU - Clerici, Mario

AU - Cooke, Anne

AU - Cosma, Antonio

AU - Cosmi, Lorenzo

AU - Cumano, Ana

AU - Dang, Van Duc

AU - Davies, Derek

AU - De Biasi, Sara

AU - Del Zotto, Genny

AU - Della Bella, Silvia

AU - Dellabona, Paolo

AU - Deniz, Günnur

AU - Dessing, Mark

AU - Diefenbach, Andreas

AU - Di Santo, James

AU - Dieli, Francesco

AU - Dolf, Andreas

AU - Donnenberg, Vera S

AU - Dörner, Thomas

AU - Ehrhardt, Götz R A

AU - Lugli, Enrico

AU - Matarese, Giuseppe

AU - Moretta, Lorenzo

AU - Rubartelli, Anna

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KW - Immunologic Techniques

KW - Immunophenotyping

KW - Quality Control

KW - RNA

KW - Research Design

KW - Software

KW - T-Lymphocytes

KW - Journal Article

KW - Research Support, N.I.H., Extramural

KW - Research Support, Non-U.S. Gov't

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Access to Document

10.1002/eji.201646632