H-2 class I gene analysis in 3-methylcholanthrene-induced murine fibrosarcomas

C. Castelli, F. Mazzucchelli, M. Sensi, G. Carbone, G. Parmiani

Research output: Contribution to journalArticlepeer-review

Abstract

The objective of our study was to determine whether and how frequently MHC class I gene alterations occur in fibrosarcomas induced in BALB/c mice by the carcinogen 3-methylcholanthrene (3-MCA). Southern blot was performed to analyze the genomic organization of class I genes in a panel of twenty tumors of different immunogenic strenght used at early in vivo passages. Our results rule out the presence of gross rearrangements or deletions in the H-2 class I genes of the tumors tested and indicate that the frequency of detectable class I alterations giving rise to restriction fragments length polymorphism (RFLP) should be very low.

Original languageEnglish
Pages (from-to)129-135
Number of pages7
JournalExperimental and Clinical Immunogenetics
Volume7
Issue number2
Publication statusPublished - 1990

ASJC Scopus subject areas

  • Immunology
  • Genetics(clinical)
  • Genetics

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