H1° and H3.3B mRNA levels in developing rat brain

Daniele Castiglia, Alessandro Cestelli, Maria Scaturro, Tommaso Nastasi, Italia Di Liegro

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Two overlapping rat cDNAs, covering a continuous region of 1107 base pairs, have been isolated and sequenced. The clones contain identical open reading frames, encoding a 136 amino acid long polypeptide which exhibits 100% identity to other mammalian H3.3 histone variants. We show that the inserts derive, in particular, from the H3.3B gene. We used these inserts and an insert from an H1° encoding clone, previously described (6), as probes to study the accumulation of mRNAs encoding the corresponding histone replacement variants (namely, H1° and H3.3) during rat brain development. We found that the concentration of both H1° and H3.3B mRNAs decreases from the embryonal day 18 (E18) to the postnatal day 10 (P10), with inverse correlation to protein accumulation.

Original languageEnglish
Pages (from-to)1531-1537
Number of pages7
JournalNeurochemical Research
Volume19
Issue number12
DOIs
Publication statusPublished - Dec 1994

Fingerprint

Histones
Rats
Brain
Clone Cells
Messenger RNA
Base Pairing
Open Reading Frames
Complementary DNA
Genes
Amino Acids
Peptides
Proteins

Keywords

  • chromatin
  • Histone H1°
  • histones H 3.3
  • rat brain development

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

Cite this

Castiglia, D., Cestelli, A., Scaturro, M., Nastasi, T., & Di Liegro, I. (1994). H1° and H3.3B mRNA levels in developing rat brain. Neurochemical Research, 19(12), 1531-1537. https://doi.org/10.1007/BF00969002

H1° and H3.3B mRNA levels in developing rat brain. / Castiglia, Daniele; Cestelli, Alessandro; Scaturro, Maria; Nastasi, Tommaso; Di Liegro, Italia.

In: Neurochemical Research, Vol. 19, No. 12, 12.1994, p. 1531-1537.

Research output: Contribution to journalArticle

Castiglia, D, Cestelli, A, Scaturro, M, Nastasi, T & Di Liegro, I 1994, 'H1° and H3.3B mRNA levels in developing rat brain', Neurochemical Research, vol. 19, no. 12, pp. 1531-1537. https://doi.org/10.1007/BF00969002
Castiglia D, Cestelli A, Scaturro M, Nastasi T, Di Liegro I. H1° and H3.3B mRNA levels in developing rat brain. Neurochemical Research. 1994 Dec;19(12):1531-1537. https://doi.org/10.1007/BF00969002
Castiglia, Daniele ; Cestelli, Alessandro ; Scaturro, Maria ; Nastasi, Tommaso ; Di Liegro, Italia. / H1° and H3.3B mRNA levels in developing rat brain. In: Neurochemical Research. 1994 ; Vol. 19, No. 12. pp. 1531-1537.
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