H1° and H3.3B mRNA levels in developing rat brain

Daniele Castiglia, Alessandro Cestelli, Maria Scaturro, Tommaso Nastasi, Italia Di Liegro

Research output: Contribution to journalArticlepeer-review


Two overlapping rat cDNAs, covering a continuous region of 1107 base pairs, have been isolated and sequenced. The clones contain identical open reading frames, encoding a 136 amino acid long polypeptide which exhibits 100% identity to other mammalian H3.3 histone variants. We show that the inserts derive, in particular, from the H3.3B gene. We used these inserts and an insert from an H1° encoding clone, previously described (6), as probes to study the accumulation of mRNAs encoding the corresponding histone replacement variants (namely, H1° and H3.3) during rat brain development. We found that the concentration of both H1° and H3.3B mRNAs decreases from the embryonal day 18 (E18) to the postnatal day 10 (P10), with inverse correlation to protein accumulation.

Original languageEnglish
Pages (from-to)1531-1537
Number of pages7
JournalNeurochemical Research
Issue number12
Publication statusPublished - Dec 1994


  • chromatin
  • Histone H1°
  • histones H 3.3
  • rat brain development

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry


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