TY - JOUR
T1 - H1° and H3.3B mRNA levels in developing rat brain
AU - Castiglia, Daniele
AU - Cestelli, Alessandro
AU - Scaturro, Maria
AU - Nastasi, Tommaso
AU - Di Liegro, Italia
PY - 1994/12
Y1 - 1994/12
N2 - Two overlapping rat cDNAs, covering a continuous region of 1107 base pairs, have been isolated and sequenced. The clones contain identical open reading frames, encoding a 136 amino acid long polypeptide which exhibits 100% identity to other mammalian H3.3 histone variants. We show that the inserts derive, in particular, from the H3.3B gene. We used these inserts and an insert from an H1° encoding clone, previously described (6), as probes to study the accumulation of mRNAs encoding the corresponding histone replacement variants (namely, H1° and H3.3) during rat brain development. We found that the concentration of both H1° and H3.3B mRNAs decreases from the embryonal day 18 (E18) to the postnatal day 10 (P10), with inverse correlation to protein accumulation.
AB - Two overlapping rat cDNAs, covering a continuous region of 1107 base pairs, have been isolated and sequenced. The clones contain identical open reading frames, encoding a 136 amino acid long polypeptide which exhibits 100% identity to other mammalian H3.3 histone variants. We show that the inserts derive, in particular, from the H3.3B gene. We used these inserts and an insert from an H1° encoding clone, previously described (6), as probes to study the accumulation of mRNAs encoding the corresponding histone replacement variants (namely, H1° and H3.3) during rat brain development. We found that the concentration of both H1° and H3.3B mRNAs decreases from the embryonal day 18 (E18) to the postnatal day 10 (P10), with inverse correlation to protein accumulation.
KW - chromatin
KW - Histone H1°
KW - histones H 3.3
KW - rat brain development
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U2 - 10.1007/BF00969002
DO - 10.1007/BF00969002
M3 - Article
C2 - 7877725
AN - SCOPUS:0028253975
VL - 19
SP - 1531
EP - 1537
JO - Neurochemical Research
JF - Neurochemical Research
SN - 0364-3190
IS - 12
ER -