H1° and H3.3B mRNA levels in developing rat brain

Daniele Castiglia; Alessandro Cestelli; Maria Scaturro; Tommaso Nastasi; Italia Di Liegro

doi:10.1007/BF00969002

H1° and H3.3B mRNA levels in developing rat brain

Daniele Castiglia, Alessandro Cestelli, Maria Scaturro, Tommaso Nastasi, Italia Di Liegro

Istituto Dermopatico dell'Immacolata , IDI

Research output: Contribution to journal › Article › peer-review

Abstract

Two overlapping rat cDNAs, covering a continuous region of 1107 base pairs, have been isolated and sequenced. The clones contain identical open reading frames, encoding a 136 amino acid long polypeptide which exhibits 100% identity to other mammalian H3.3 histone variants. We show that the inserts derive, in particular, from the H3.3B gene. We used these inserts and an insert from an H1° encoding clone, previously described (6), as probes to study the accumulation of mRNAs encoding the corresponding histone replacement variants (namely, H1° and H3.3) during rat brain development. We found that the concentration of both H1° and H3.3B mRNAs decreases from the embryonal day 18 (E18) to the postnatal day 10 (P10), with inverse correlation to protein accumulation.

Original language	English
Pages (from-to)	1531-1537
Number of pages	7
Journal	Neurochemical Research
Volume	19
Issue number	12
DOIs	https://doi.org/10.1007/BF00969002
Publication status	Published - Dec 1994

Keywords

chromatin
Histone H1°
histones H 3.3
rat brain development

ASJC Scopus subject areas

Neuroscience(all)
Biochemistry

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10.1007/BF00969002

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title = "H1° and H3.3B mRNA levels in developing rat brain",

abstract = "Two overlapping rat cDNAs, covering a continuous region of 1107 base pairs, have been isolated and sequenced. The clones contain identical open reading frames, encoding a 136 amino acid long polypeptide which exhibits 100% identity to other mammalian H3.3 histone variants. We show that the inserts derive, in particular, from the H3.3B gene. We used these inserts and an insert from an H1° encoding clone, previously described (6), as probes to study the accumulation of mRNAs encoding the corresponding histone replacement variants (namely, H1° and H3.3) during rat brain development. We found that the concentration of both H1° and H3.3B mRNAs decreases from the embryonal day 18 (E18) to the postnatal day 10 (P10), with inverse correlation to protein accumulation.",

keywords = "chromatin, Histone H1°, histones H 3.3, rat brain development",

author = "Daniele Castiglia and Alessandro Cestelli and Maria Scaturro and Tommaso Nastasi and {Di Liegro}, Italia",

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AU - Castiglia, Daniele

AU - Cestelli, Alessandro

AU - Scaturro, Maria

AU - Nastasi, Tommaso

AU - Di Liegro, Italia

PY - 1994/12

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