H9c2 cardiac myoblasts undergo apoptosis in a model of ischemia consisting of serum deprivation and hypoxia: Inhibition by PMA

Francesca Bonavita, Claudio Stefanelli, Emanuele Giordano, Marta Columbaro, Annalisa Facchini, Francesca Bonafè, Claudio Marcello Caldarera, Carlo Guarnieri

Research output: Contribution to journalArticlepeer-review

Abstract

Cardiac myocytes undergo apoptosis under condition of ischemia. Little is known, however, about the molecular pathways that mediate this response. We show that serum deprivation and hypoxia, components of ischemia in vivo, resulted in apoptosis of rat ventricular myoblast cells H9c2. Hypoxia alone did not induce significant apoptosis for at least 48 h, but largely increased the proapoptotic action of serum deprivation. H9c2 cells apoptosis is evidenced by an increase in terminal (TdT)-mediated dUTP nick end-labeling-positive nuclei and by activation of caspases 3, 6, 7 and 9, and loss of mitochondrial functions. In this model of simulated ischemia, represented by serum deprivation plus hypoxia, cardiomyoblasts apoptosis was associated with a p53-independent Bax accumulation and with a down-regulation of Bcl-xL, whereas the levels of cIAP-1, cIAP-2 and X-IAP proteins did not change. Phorbol-12-myristate-13-acetate significantly reduced the induction of apoptosis, inhibiting caspase 3 cleavage, Bax accumulation, Bcl-xL down-regulation as well as restoring cell viability.

Original languageEnglish
Pages (from-to)85-91
Number of pages7
JournalFEBS Letters
Volume536
Issue number1-3
DOIs
Publication statusPublished - Feb 11 2003

Keywords

  • Apoptosis
  • Bcl-2 family proteins
  • Caspase
  • H9c2
  • Ischemia
  • Phorbol-12-myristate-13-acetate

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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