Hadron Therapy, Magnetic Nanoparticles and Hyperthermia: A Promising Combined Tool for Pancreatic Cancer Treatment.

Francesca Brero, Martin Albino, Antonio Antoccia, Paolo Arosio, Matteo Avolio, Francesco Berardinelli, Daniela Bettega, Paola Calzolari, Mario Ciocca, Maurizio Corti, Angelica Facoetti, Salvatore Gallo, Flavia Groppi, Andrea Guerrini, Claudia Innocenti, Cristina Lenardi, Silvia Locarno, Simone Manenti, Renato Marchesini, Manuel MarianiFrancesco Orsini, Emanuele Pignoli, Claudio Sangregorio, Ivan Veronese, Alessandro Lascialfari

Research output: Contribution to journalArticlepeer-review


A combination of carbon ions/photons irradiation and hyperthermia as a novel therapeutic approach for the in-vitro treatment of pancreatic cancer BxPC3 cells is presented. The radiation doses used are 0-2 Gy for carbon ions and 0-7 Gy for 6 MV photons. Hyperthermia is realized via a standard heating bath, assisted by magnetic fluid hyperthermia (MFH) that utilizes magnetic nanoparticles (MNPs) exposed to an alternating magnetic field of amplitude 19.5 mTesla and frequency 109.8 kHz. Starting from 37 °C, the temperature is gradually increased and the sample is kept at 42 °C for 30 min. For MFH, MNPs with a mean diameter of 19 nm and specific absorption rate of 110 ± 30 W/g(Fe3)o(4) coated with a biocompatible ligand to ensure stability in physiological media are used. Irradiation diminishes the clonogenic survival at an extent that depends on the radiation type, and its decrease is amplified both by the MNPs cellular uptake and the hyperthermia protocol. Significant increases in DNA double-strand breaks at 6 h are observed in samples exposed to MNP uptake, treated with 0.75 Gy carbon-ion irradiation and hyperthermia. The proposed experimental protocol, based on the combination of hadron irradiation and hyperthermia, represents a first step towards an innovative clinical option for pancreatic cancer.
Original languageEnglish
Issue number10
Publication statusPublished - Sep 1 2020


  • pancreatic cancer
  • hyperthermia
  • hadron therapy
  • magnetic fluid hyperthermia
  • magnetic nanoparticles
  • nanomaterials


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