Haemopoietic CD34+ progenitor cells are not infected by HIV-1 in vivo but show impaired clonogenesis

A. De Luca, L. Teofili, A. Antinori, M. S. Iovino, P. Mencarini, E. Visconti, E. Tamburrini, G. Leone, L. Ortona

Research output: Contribution to journalArticle

Abstract

We evaluated the role of CD34+ bone marrow progenitor cells in vivo, in the pathogenesis of AIDS-related haematological abnormalities. The clonogenic activity of CD34+ cells from seven patients with HIV-1 infection, without bone marrow involving opportunistic infections or neoplasms, was assessed in semisolid cultures. The number of CFU-GM was significantly reduced as compared to the controls (P = 0.017), independently from myelotoxic therapy, while the number of BFU-E was not. The presence of retroviral sequences in CFU-GM colonies from four patients and in the total population of CD34+ cells from six patients with advanced stage HIV infection was investigated using the polymerase chain reaction. The presence of HIV-1 sequences was also searched for in a purified suspension of CD34+ cells after 3 weeks liquid culture. All these cells were always HIV-1 negative, while viral sequences were always detected in bone marrow mononuclear cells from these and other patients. The number of HIV-1 DNA copies decreased with increasing enrichment. At most 1:10000 CD34+ cells are infected in vivo. Other mechanisms than direct viral infection of progenitor cells must account for the defective haemopoiesis in HIV-1 infected patients.

Original languageEnglish
Pages (from-to)20-24
Number of pages5
JournalBritish Journal of Haematology
Volume85
Issue number1
Publication statusPublished - 1993

ASJC Scopus subject areas

  • Hematology

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    De Luca, A., Teofili, L., Antinori, A., Iovino, M. S., Mencarini, P., Visconti, E., Tamburrini, E., Leone, G., & Ortona, L. (1993). Haemopoietic CD34+ progenitor cells are not infected by HIV-1 in vivo but show impaired clonogenesis. British Journal of Haematology, 85(1), 20-24.