Abstract
Human T lymphocytes, after phytohaemagglutinin (PHA) stimulation, are able to develop colonies in semisolid agar culture. Adherent cells (AC) or their conditioned media are necessary in order to produce a proliferative response of T cells in this experimental model. Previous investigation using this method demonstrated a defective function of AC in primary biliary cirrhosis, an autoimmune liver disease. Recent reports suggested a possible pathogenetic role of monocytes in Hashimoto's disease (HD). This aspect of AC function was studied in 6 patients and in 6 healthy controls. The number of colonies developed by T lymphocytes in HD patients did not differ from that of controls; in addition the depletion of AC in both groups resulted in a sharp decrease in colony development, which could be restored by AC from normal or HD subjects. These experiments indicate the normal capability of HD - AC to react with T lymphocytes in the proliferative response to PHA, but does not exclude their possible pathogenetic role in this disease.
| Original language | English |
|---|---|
| Pages (from-to) | 1085-1086 |
| Number of pages | 2 |
| Journal | IRCS Medical Science |
| Volume | 9 |
| Issue number | 12 |
| Publication status | Published - 1981 |
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ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Medicine(all)
Cite this
Hashimoto's thyroiditis : Influence of adherent cells on T lymphocytes colony growth. / Ferrini, S.; Bagnasco, M.; Ramarli, D.
In: IRCS Medical Science, Vol. 9, No. 12, 1981, p. 1085-1086.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Hashimoto's thyroiditis
T2 - Influence of adherent cells on T lymphocytes colony growth
AU - Ferrini, S.
AU - Bagnasco, M.
AU - Ramarli, D.
PY - 1981
Y1 - 1981
N2 - Human T lymphocytes, after phytohaemagglutinin (PHA) stimulation, are able to develop colonies in semisolid agar culture. Adherent cells (AC) or their conditioned media are necessary in order to produce a proliferative response of T cells in this experimental model. Previous investigation using this method demonstrated a defective function of AC in primary biliary cirrhosis, an autoimmune liver disease. Recent reports suggested a possible pathogenetic role of monocytes in Hashimoto's disease (HD). This aspect of AC function was studied in 6 patients and in 6 healthy controls. The number of colonies developed by T lymphocytes in HD patients did not differ from that of controls; in addition the depletion of AC in both groups resulted in a sharp decrease in colony development, which could be restored by AC from normal or HD subjects. These experiments indicate the normal capability of HD - AC to react with T lymphocytes in the proliferative response to PHA, but does not exclude their possible pathogenetic role in this disease.
AB - Human T lymphocytes, after phytohaemagglutinin (PHA) stimulation, are able to develop colonies in semisolid agar culture. Adherent cells (AC) or their conditioned media are necessary in order to produce a proliferative response of T cells in this experimental model. Previous investigation using this method demonstrated a defective function of AC in primary biliary cirrhosis, an autoimmune liver disease. Recent reports suggested a possible pathogenetic role of monocytes in Hashimoto's disease (HD). This aspect of AC function was studied in 6 patients and in 6 healthy controls. The number of colonies developed by T lymphocytes in HD patients did not differ from that of controls; in addition the depletion of AC in both groups resulted in a sharp decrease in colony development, which could be restored by AC from normal or HD subjects. These experiments indicate the normal capability of HD - AC to react with T lymphocytes in the proliferative response to PHA, but does not exclude their possible pathogenetic role in this disease.
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M3 - Article
VL - 9
SP - 1085
EP - 1086
JO - IRCS Medical Science
JF - IRCS Medical Science
SN - 0305-6651
IS - 12
ER -