Human T lymphocytes, after phytohaemagglutinin (PHA) stimulation, are able to develop colonies in semisolid agar culture. Adherent cells (AC) or their conditioned media are necessary in order to produce a proliferative response of T cells in this experimental model. Previous investigation using this method demonstrated a defective function of AC in primary biliary cirrhosis, an autoimmune liver disease. Recent reports suggested a possible pathogenetic role of monocytes in Hashimoto's disease (HD). This aspect of AC function was studied in 6 patients and in 6 healthy controls. The number of colonies developed by T lymphocytes in HD patients did not differ from that of controls; in addition the depletion of AC in both groups resulted in a sharp decrease in colony development, which could be restored by AC from normal or HD subjects. These experiments indicate the normal capability of HD - AC to react with T lymphocytes in the proliferative response to PHA, but does not exclude their possible pathogenetic role in this disease.
|Number of pages||2|
|Journal||IRCS Medical Science|
|Publication status||Published - 1981|
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)