TY - JOUR
T1 - HDAC8 regulates canonical Wnt pathway to promote differentiation in skeletal muscles
AU - Ferrari, Luca
AU - Bragato, Cinzia
AU - Brioschi, Loredana
AU - Spreafico, Marco
AU - Esposito, Simona
AU - Pezzotta, Alex
AU - Pizzetti, Fabrizio
AU - Moreno-Fortuny, Artal
AU - Bellipanni, Gianfranco
AU - Giordano, Antonio
AU - Riva, Paola
AU - Frabetti, Flavia
AU - Viani, Paola
AU - Cossu, Giulio
AU - Mora, Marina
AU - Marozzi, Anna
AU - Pistocchi, Anna
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Histone deacetylase 8 (HDAC8) is a class 1 histone deacetylase and a member of the cohesin complex. HDAC8 is expressed in smooth muscles, but its expression in skeletal muscle has not been described. We have shown for the first time that HDAC8 is expressed in human and zebrafish skeletal muscles. Using RD/12 and RD/18 rhabdomyosarcoma cells with low and high differentiation potency, respectively, we highlighted a specific correlation with HDAC8 expression and an advanced stage of muscle differentiation. We inhibited HDAC8 activity through a specific PCI-34051 inhibitor in murine C2C12 myoblasts and zebrafish embryos, and we observed skeletal muscles differentiation impairment. We also found a positive regulation of the canonical Wnt signaling by HDAC8 that might explain muscle differentiation defects. These findings suggest a novel mechanism through which HDAC8 expression, in a specific time window of skeletal muscle development, positively regulates canonical Wnt pathway that is necessary for muscle differentiation.
AB - Histone deacetylase 8 (HDAC8) is a class 1 histone deacetylase and a member of the cohesin complex. HDAC8 is expressed in smooth muscles, but its expression in skeletal muscle has not been described. We have shown for the first time that HDAC8 is expressed in human and zebrafish skeletal muscles. Using RD/12 and RD/18 rhabdomyosarcoma cells with low and high differentiation potency, respectively, we highlighted a specific correlation with HDAC8 expression and an advanced stage of muscle differentiation. We inhibited HDAC8 activity through a specific PCI-34051 inhibitor in murine C2C12 myoblasts and zebrafish embryos, and we observed skeletal muscles differentiation impairment. We also found a positive regulation of the canonical Wnt signaling by HDAC8 that might explain muscle differentiation defects. These findings suggest a novel mechanism through which HDAC8 expression, in a specific time window of skeletal muscle development, positively regulates canonical Wnt pathway that is necessary for muscle differentiation.
KW - histone deacetylase 8 (HDAC8)
KW - rhabdomyosarcoma
KW - skeletal muscle
KW - Wnt
KW - zebrafish
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U2 - 10.1002/jcp.27341
DO - 10.1002/jcp.27341
M3 - Article
C2 - 30246374
AN - SCOPUS:85053690555
JO - Journal of cellular and comparative physiology
JF - Journal of cellular and comparative physiology
SN - 0021-9541
ER -