Abstract
Classic methods to determine human red blood cell (RBC) antigens are based on serologic testing. Thanks to increased knowledge of the molecular basis associated with many blood group antigens, it is currently possible to predict their presence or absence on the red cell membrane. Several molecular techniques have been developed to detect the most important allelic variations attributable to single nucleotide polymorphisms. The human erythrocyte antigen (HEA) BeadChip™ system manufactured by BioArray Solutions (Immucor, Warren, NJ) is one of the commercial DNA array platforms currently available to predict HEAs by DNA analysis. This technology provides a useful tool to increase the inventory of antigen-negative RBC units and prevent immunization of patients who require chronic transfusion by providing compatible RBC units based on matching by DNA testing.
| Original language | English |
|---|---|
| Pages (from-to) | 81-90 |
| Number of pages | 10 |
| Journal | Immunohematology |
| Volume | 31 |
| Issue number | 2 |
| Publication status | Published - 2015 |
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ASJC Scopus subject areas
- Immunology and Allergy
- Medicine(all)
- Hematology
Cite this
HEA BeadChip™ technology in immunohematology. / Paccapelo, Cinzia; Truglio, Francesca; Antonietta Villa, Maria; Revelli, Nicoletta; Marconi, Maurizio.
In: Immunohematology, Vol. 31, No. 2, 2015, p. 81-90.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - HEA BeadChip™ technology in immunohematology
AU - Paccapelo, Cinzia
AU - Truglio, Francesca
AU - Antonietta Villa, Maria
AU - Revelli, Nicoletta
AU - Marconi, Maurizio
PY - 2015
Y1 - 2015
N2 - Classic methods to determine human red blood cell (RBC) antigens are based on serologic testing. Thanks to increased knowledge of the molecular basis associated with many blood group antigens, it is currently possible to predict their presence or absence on the red cell membrane. Several molecular techniques have been developed to detect the most important allelic variations attributable to single nucleotide polymorphisms. The human erythrocyte antigen (HEA) BeadChip™ system manufactured by BioArray Solutions (Immucor, Warren, NJ) is one of the commercial DNA array platforms currently available to predict HEAs by DNA analysis. This technology provides a useful tool to increase the inventory of antigen-negative RBC units and prevent immunization of patients who require chronic transfusion by providing compatible RBC units based on matching by DNA testing.
AB - Classic methods to determine human red blood cell (RBC) antigens are based on serologic testing. Thanks to increased knowledge of the molecular basis associated with many blood group antigens, it is currently possible to predict their presence or absence on the red cell membrane. Several molecular techniques have been developed to detect the most important allelic variations attributable to single nucleotide polymorphisms. The human erythrocyte antigen (HEA) BeadChip™ system manufactured by BioArray Solutions (Immucor, Warren, NJ) is one of the commercial DNA array platforms currently available to predict HEAs by DNA analysis. This technology provides a useful tool to increase the inventory of antigen-negative RBC units and prevent immunization of patients who require chronic transfusion by providing compatible RBC units based on matching by DNA testing.
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M3 - Article
C2 - 26495894
AN - SCOPUS:84982985653
VL - 31
SP - 81
EP - 90
JO - Immunohematology
JF - Immunohematology
SN - 0894-203X
IS - 2
ER -