Heart valve scaffold fabrication: Bioinspired control of macro-scale morphology, mechanics and micro-structure

Antonio D'Amore, Samuel K. Luketich, Giuseppe M. Raffa, Salim Olia, Giorgio Menallo, Antonino Mazzola, Flavio D'Accardi, Tamir Grunberg, Xinzhu Gu, Michele Pilato, Marina V. Kameneva, Vinay Badhwar, William R. Wagner

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Valvular heart disease is currently treated with mechanical valves, which benefit from longevity, but are burdened by chronic anticoagulation therapy, or with bioprosthetic valves, which have reduced thromboembolic risk, but limited durability. Tissue engineered heart valves have been proposed to resolve these issues by implanting a scaffold that is replaced by endogenous growth, leaving autologous, functional leaflets that would putatively eliminate the need for anticoagulation and avoid calcification. Despite the diversity in fabrication strategies and encouraging results in large animal models, control over engineered valve structure-function remains at best partial. This study aimed to overcome these limitations by introducing double component deposition (DCD), an electrodeposition technique that employs multi-phase electrodes to dictate valve macro and microstructure and resultant function. Results in this report demonstrate the capacity of the DCD method to simultaneously control scaffold macro-scale morphology, mechanics and microstructure while producing fully assembled stent-less multi-leaflet valves composed of microscopic fibers. DCD engineered valve characterization included: leaflet thickness, biaxial properties, bending properties, and quantitative structural analysis of multi-photon and scanning electron micrographs. Quasi-static ex-vivo valve coaptation testing and dynamic organ level functional assessment in a pressure pulse duplicating device demonstrated appropriate acute valve functionality.

Original languageEnglish
Pages (from-to)25-37
Number of pages13
JournalBiomaterials
Volume150
DOIs
Publication statusPublished - Jan 1 2018

Fingerprint

Electroplating
Heart Valve Diseases
Heart Valves
Mechanics
Photons
Scaffolds
Stents
Macros
Electron tubes
Electrodes
Animal Models
Electrons
Blood Pressure
Fabrication
Equipment and Supplies
Microstructure
Growth
Functional assessment
Electrodeposition
Structural analysis

Keywords

  • Aortic
  • Bending mechanics
  • Biaxial mechanics
  • Electrodeposition
  • Electrospinning
  • Mitral
  • Pulmonary heart valve structure
  • Tissue engineered heart valve
  • Tricuspid

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Heart valve scaffold fabrication : Bioinspired control of macro-scale morphology, mechanics and micro-structure. / D'Amore, Antonio; Luketich, Samuel K.; Raffa, Giuseppe M.; Olia, Salim; Menallo, Giorgio; Mazzola, Antonino; D'Accardi, Flavio; Grunberg, Tamir; Gu, Xinzhu; Pilato, Michele; Kameneva, Marina V.; Badhwar, Vinay; Wagner, William R.

In: Biomaterials, Vol. 150, 01.01.2018, p. 25-37.

Research output: Contribution to journalArticle

D'Amore, A, Luketich, SK, Raffa, GM, Olia, S, Menallo, G, Mazzola, A, D'Accardi, F, Grunberg, T, Gu, X, Pilato, M, Kameneva, MV, Badhwar, V & Wagner, WR 2018, 'Heart valve scaffold fabrication: Bioinspired control of macro-scale morphology, mechanics and micro-structure', Biomaterials, vol. 150, pp. 25-37. https://doi.org/10.1016/j.biomaterials.2017.10.011
D'Amore, Antonio ; Luketich, Samuel K. ; Raffa, Giuseppe M. ; Olia, Salim ; Menallo, Giorgio ; Mazzola, Antonino ; D'Accardi, Flavio ; Grunberg, Tamir ; Gu, Xinzhu ; Pilato, Michele ; Kameneva, Marina V. ; Badhwar, Vinay ; Wagner, William R. / Heart valve scaffold fabrication : Bioinspired control of macro-scale morphology, mechanics and micro-structure. In: Biomaterials. 2018 ; Vol. 150. pp. 25-37.
@article{586f44e22a7d4c11b3c5cf4aef59f059,
title = "Heart valve scaffold fabrication: Bioinspired control of macro-scale morphology, mechanics and micro-structure",
abstract = "Valvular heart disease is currently treated with mechanical valves, which benefit from longevity, but are burdened by chronic anticoagulation therapy, or with bioprosthetic valves, which have reduced thromboembolic risk, but limited durability. Tissue engineered heart valves have been proposed to resolve these issues by implanting a scaffold that is replaced by endogenous growth, leaving autologous, functional leaflets that would putatively eliminate the need for anticoagulation and avoid calcification. Despite the diversity in fabrication strategies and encouraging results in large animal models, control over engineered valve structure-function remains at best partial. This study aimed to overcome these limitations by introducing double component deposition (DCD), an electrodeposition technique that employs multi-phase electrodes to dictate valve macro and microstructure and resultant function. Results in this report demonstrate the capacity of the DCD method to simultaneously control scaffold macro-scale morphology, mechanics and microstructure while producing fully assembled stent-less multi-leaflet valves composed of microscopic fibers. DCD engineered valve characterization included: leaflet thickness, biaxial properties, bending properties, and quantitative structural analysis of multi-photon and scanning electron micrographs. Quasi-static ex-vivo valve coaptation testing and dynamic organ level functional assessment in a pressure pulse duplicating device demonstrated appropriate acute valve functionality.",
keywords = "Aortic, Bending mechanics, Biaxial mechanics, Electrodeposition, Electrospinning, Mitral, Pulmonary heart valve structure, Tissue engineered heart valve, Tricuspid",
author = "Antonio D'Amore and Luketich, {Samuel K.} and Raffa, {Giuseppe M.} and Salim Olia and Giorgio Menallo and Antonino Mazzola and Flavio D'Accardi and Tamir Grunberg and Xinzhu Gu and Michele Pilato and Kameneva, {Marina V.} and Vinay Badhwar and Wagner, {William R.}",
year = "2018",
month = "1",
day = "1",
doi = "10.1016/j.biomaterials.2017.10.011",
language = "English",
volume = "150",
pages = "25--37",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Heart valve scaffold fabrication

T2 - Bioinspired control of macro-scale morphology, mechanics and micro-structure

AU - D'Amore, Antonio

AU - Luketich, Samuel K.

AU - Raffa, Giuseppe M.

AU - Olia, Salim

AU - Menallo, Giorgio

AU - Mazzola, Antonino

AU - D'Accardi, Flavio

AU - Grunberg, Tamir

AU - Gu, Xinzhu

AU - Pilato, Michele

AU - Kameneva, Marina V.

AU - Badhwar, Vinay

AU - Wagner, William R.

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Valvular heart disease is currently treated with mechanical valves, which benefit from longevity, but are burdened by chronic anticoagulation therapy, or with bioprosthetic valves, which have reduced thromboembolic risk, but limited durability. Tissue engineered heart valves have been proposed to resolve these issues by implanting a scaffold that is replaced by endogenous growth, leaving autologous, functional leaflets that would putatively eliminate the need for anticoagulation and avoid calcification. Despite the diversity in fabrication strategies and encouraging results in large animal models, control over engineered valve structure-function remains at best partial. This study aimed to overcome these limitations by introducing double component deposition (DCD), an electrodeposition technique that employs multi-phase electrodes to dictate valve macro and microstructure and resultant function. Results in this report demonstrate the capacity of the DCD method to simultaneously control scaffold macro-scale morphology, mechanics and microstructure while producing fully assembled stent-less multi-leaflet valves composed of microscopic fibers. DCD engineered valve characterization included: leaflet thickness, biaxial properties, bending properties, and quantitative structural analysis of multi-photon and scanning electron micrographs. Quasi-static ex-vivo valve coaptation testing and dynamic organ level functional assessment in a pressure pulse duplicating device demonstrated appropriate acute valve functionality.

AB - Valvular heart disease is currently treated with mechanical valves, which benefit from longevity, but are burdened by chronic anticoagulation therapy, or with bioprosthetic valves, which have reduced thromboembolic risk, but limited durability. Tissue engineered heart valves have been proposed to resolve these issues by implanting a scaffold that is replaced by endogenous growth, leaving autologous, functional leaflets that would putatively eliminate the need for anticoagulation and avoid calcification. Despite the diversity in fabrication strategies and encouraging results in large animal models, control over engineered valve structure-function remains at best partial. This study aimed to overcome these limitations by introducing double component deposition (DCD), an electrodeposition technique that employs multi-phase electrodes to dictate valve macro and microstructure and resultant function. Results in this report demonstrate the capacity of the DCD method to simultaneously control scaffold macro-scale morphology, mechanics and microstructure while producing fully assembled stent-less multi-leaflet valves composed of microscopic fibers. DCD engineered valve characterization included: leaflet thickness, biaxial properties, bending properties, and quantitative structural analysis of multi-photon and scanning electron micrographs. Quasi-static ex-vivo valve coaptation testing and dynamic organ level functional assessment in a pressure pulse duplicating device demonstrated appropriate acute valve functionality.

KW - Aortic

KW - Bending mechanics

KW - Biaxial mechanics

KW - Electrodeposition

KW - Electrospinning

KW - Mitral

KW - Pulmonary heart valve structure

KW - Tissue engineered heart valve

KW - Tricuspid

UR - http://www.scopus.com/inward/record.url?scp=85031014974&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85031014974&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2017.10.011

DO - 10.1016/j.biomaterials.2017.10.011

M3 - Article

AN - SCOPUS:85031014974

VL - 150

SP - 25

EP - 37

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

ER -