Heat shock induces the transcriptional activation of c-fos protooncogene

F. Colotta, N. Polentarutti, M. Staffico, G. Fincato, A. Mantovani

Research output: Contribution to journalArticle

Abstract

Treatment of murine 3T3 fibroblasts with mild elevated temperature (43°C for 45 minutes) followed by recovery at 37°C induced high levels of c-fos mRNA. The maximal c-fos induction was found after recovery at 37°C for 15 minutes. Sodium arsenite induced both hsp 70 and c-fos transcripts. Induction of hsp 70 and c-fos by heat shock did not require intact protein synthesis. c-fos mRNA induced by heat shock was more stable ( T1 2> 90 minutes) than that induced by phorbol esters ( t1 2∼30 minutes). Northern blot analysis in the presence of actinomycin D and nuclear run off experiments demonstrated that heat shock augmented the transcription rate of c-fos protooncogene. Human growth hormone under the control of a murine genomic c-fos fragment spanning 770 bp 5′ from the start of transcription is induced in transfected cells in response to heat shock. These data indicate that heat shock induces c-fos protooncogene acting at both transcriptional and post-transcriptional (i.e. via stabilization of transcripts) levels.

Original languageEnglish
Pages (from-to)1013-1019
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume168
Issue number3
DOIs
Publication statusPublished - May 16 1990

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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