Heat shock proteins and autoimmunity in patients with carotid atherosclerosis

Rachele Riganò, Elisabetta Profumo, Brigitta Buttari, Angela Tagliani, Linda Petrone, Giulia D'Amati, Flora Ippoliti, Raffaele Capoano, Lorenzo Fumagalli, Bruno Salvati, Rita Businaro

Research output: Chapter in Book/Report/Conference proceedingConference contribution


Studies aimed at elucidating the pathogenetic mechanisms underlying the initiation and progression of human atherosclerosis have emphasized the central role of inflammatory and immune cells. Atherosclerotic plaques are infiltrated by activated macrophages, T and B lymphocytes, plasma cells, and mast cells, releasing inflammatory molecules, which amplify the severity of the disease. Endothelial cells subjected to various stress conditions express increased amounts of heat shock proteins (HSPs), some of the most successfully conserved proteins throughout evolution. Many experimental observations reviewed in this article draw attention to several HSPs targeted by a specific cellular and humoral immune response in patients with atherosclerotic disease. The review also reports preliminary data obtained by our group on the possible role of HSP90 as a candidate autoantigen in carotid atherosclerosis. Our study deals with the presence of specific antibodies and T cells directed against HSP90 in patients with carotid atherosclerotic plaques. In 60% of these subjects' sera but in none of the sera from healthy controls immunoblotting (IB) detected the presence of specific antibodies. Moreover, 20% of peripheral blood mononuclear cells (PBMC) samples from patients but none from healthy subjects proliferated in response to human purified HSP90. In vitro experiments showed an upregulation of HSP90 expression in endothelial cells exposed to oxidative stress by treatment with H2O2 and greater release of soluble HSP90 in culture supernatants from H2O2-treated cells than from untreated cells.

Original languageEnglish
Title of host publicationAnnals of the New York Academy of Sciences
Number of pages10
Publication statusPublished - Jun 2007

Publication series

NameAnnals of the New York Academy of Sciences
ISSN (Print)00778923
ISSN (Electronic)17496632


  • Antibodies
  • Autoantigens
  • Carotid atherosclerosis
  • Endothelial cells
  • Heat shock proteins
  • Immune response
  • Oxidative stress
  • T lymphocytes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


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