Heat shock response by EBV-immortalized B-lymphocytes from centenarians and control subjects: A model to study the relevance of stress response in longevity

Marina Marini, Rosa Lapalombella, Silvia Canaider, Antonio Farina, Daniela Monti, Valentina De Vescovi, Marina Morellini, Dina Bellizzi, Serena Dato, Giovanna De Benedictis, Giuseppe Passarino, Raffaella Moresi, Silvia Tesei, Claudio Franceschi

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

'Successful aging', i.e. the ability to attain old age in relatively good health, is believed to be related to the capability to cope with different environmental stresses. Independently of their specific differentiation, all body cells respond to hyperthermia and other stresses with the production of Heat Shock Proteins (HSPs) that play an important role in cell survival. We investigated the heat shock response in B-lymphoid cell lines from 44 centenarians and 23 younger subjects, by studying both HSP70 synthesis and cell survival after hyperthermic treatment. Interestingly, no significant difference could be found between the two age groups as far as HSP70 synthesis was concerned; moreover, cell lines from centenarians appeared to be less prone to heat-induced apoptosis than lines from younger controls. These results, which are in contrast with previous findings showing an age-related decrease of the HSP70 synthesis and of hyperthermic response, corroborate the above mentioned hypothesis that the biological success of centenarians is due to the preservation of the capability to cope with stresses. An A/C polymorphism identified in the promoter region of HSP70-1 gene had been previously shown to affect the probability to attain longevity in females. To investigate if this effect was related to any influence of this polymorphism on HSP70 protein synthesis the correlation between A/C polymorphism and protein synthesis was investigated. We found that cells from AA centenarian females displayed a lower synthesis of HSP70.

Original languageEnglish
Pages (from-to)83-90
Number of pages8
JournalExperimental Gerontology
Volume39
Issue number1
DOIs
Publication statusPublished - Jan 2004

Fingerprint

Heat-Shock Response
Lymphocytes
Human Herpesvirus 4
B-Lymphocytes
Polymorphism
Cells
Cell Survival
Cell Line
Heat-Shock Proteins
Protein C
Genetic Promoter Regions
Fever
Age Groups
Hot Temperature
Apoptosis
Health
Proteins
Genes
Aging of materials
Therapeutics

Keywords

  • Aging
  • Apoptosis
  • Centenarians
  • DNA polymorphisms
  • EBV-immortalized cell lines
  • Heat shock
  • HSP70 (inducible)

ASJC Scopus subject areas

  • Ageing
  • Medicine(all)

Cite this

Heat shock response by EBV-immortalized B-lymphocytes from centenarians and control subjects : A model to study the relevance of stress response in longevity. / Marini, Marina; Lapalombella, Rosa; Canaider, Silvia; Farina, Antonio; Monti, Daniela; De Vescovi, Valentina; Morellini, Marina; Bellizzi, Dina; Dato, Serena; De Benedictis, Giovanna; Passarino, Giuseppe; Moresi, Raffaella; Tesei, Silvia; Franceschi, Claudio.

In: Experimental Gerontology, Vol. 39, No. 1, 01.2004, p. 83-90.

Research output: Contribution to journalArticle

Marini, M, Lapalombella, R, Canaider, S, Farina, A, Monti, D, De Vescovi, V, Morellini, M, Bellizzi, D, Dato, S, De Benedictis, G, Passarino, G, Moresi, R, Tesei, S & Franceschi, C 2004, 'Heat shock response by EBV-immortalized B-lymphocytes from centenarians and control subjects: A model to study the relevance of stress response in longevity', Experimental Gerontology, vol. 39, no. 1, pp. 83-90. https://doi.org/10.1016/j.exger.2003.09.023
Marini, Marina ; Lapalombella, Rosa ; Canaider, Silvia ; Farina, Antonio ; Monti, Daniela ; De Vescovi, Valentina ; Morellini, Marina ; Bellizzi, Dina ; Dato, Serena ; De Benedictis, Giovanna ; Passarino, Giuseppe ; Moresi, Raffaella ; Tesei, Silvia ; Franceschi, Claudio. / Heat shock response by EBV-immortalized B-lymphocytes from centenarians and control subjects : A model to study the relevance of stress response in longevity. In: Experimental Gerontology. 2004 ; Vol. 39, No. 1. pp. 83-90.
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