TY - JOUR
T1 - Heavy-metal modulation of the human intercellular adhesion molecule (ICAM-1) gene expression
AU - Martinotti, Stefano
AU - Toniato, Elena
AU - Colagrande, Antonella
AU - Alesse, Edoardo
AU - Alleva, Claudia
AU - Screpanti, Isabella
AU - Morrone, Stefania
AU - Scarpa, Susanna
AU - Frati, Luigi
AU - Hayday, Adrian C.
AU - Piovella, Franco
AU - Gulino, Alberto
PY - 1995/3/14
Y1 - 1995/3/14
N2 - The intercellular adhesion molecule 1 (ICAM-1) can be induced on many different cell types by a set of various modulators (IL1β, TNF, LPS, IFN-γ), which are released during the inflammatory process. We have investigated the possibility that other factors, related to the stress and biophysical perturbations of the inflammatory response, may also modulate ICAM-1. Here, we report that heavy metals, in particular zinc, can enhance the expression of the ICAM-1 gene on cells actively involved at different levels during inflammation. Kinetic studies of ICAM-1 gene expression shows a maximum level of induction 4 h after treatment with metals, followed by a rapid decrease to basal levels within 12 h. The effect on enhanced gene expression is mostly due to a rapid increase of the transcriptional rate as shown by nuclear run-on experiments. In B lymphoblastoid cells, but not in fibroblasts, the increase in RNA expression seems significantly greater that the subsequent increase in protein expression, suggesting that a further point of post-transcriptional regulation of ICAM-1 occurs and may be linked to the cellular specificity.
AB - The intercellular adhesion molecule 1 (ICAM-1) can be induced on many different cell types by a set of various modulators (IL1β, TNF, LPS, IFN-γ), which are released during the inflammatory process. We have investigated the possibility that other factors, related to the stress and biophysical perturbations of the inflammatory response, may also modulate ICAM-1. Here, we report that heavy metals, in particular zinc, can enhance the expression of the ICAM-1 gene on cells actively involved at different levels during inflammation. Kinetic studies of ICAM-1 gene expression shows a maximum level of induction 4 h after treatment with metals, followed by a rapid decrease to basal levels within 12 h. The effect on enhanced gene expression is mostly due to a rapid increase of the transcriptional rate as shown by nuclear run-on experiments. In B lymphoblastoid cells, but not in fibroblasts, the increase in RNA expression seems significantly greater that the subsequent increase in protein expression, suggesting that a further point of post-transcriptional regulation of ICAM-1 occurs and may be linked to the cellular specificity.
KW - (Human)
KW - Gene expression
KW - Heavy metal modulation
KW - Intercellular adhesion molecule
UR - http://www.scopus.com/inward/record.url?scp=0028915266&partnerID=8YFLogxK
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U2 - 10.1016/0167-4781(94)00237-W
DO - 10.1016/0167-4781(94)00237-W
M3 - Article
C2 - 7893746
AN - SCOPUS:0028915266
VL - 1261
SP - 107
EP - 114
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
SN - 0167-4781
IS - 1
ER -