Hedgehog signaling during expansion of human pancreatic islet-derived precursors

Rita Gallo; Fabio Arturo Grieco; Lorella Marselli; Elisabetta Ferretti; Alberto Gulino; Piero Marchetti; Francesco Dotta

doi:10.1196/annals.1447.024

Hedgehog signaling during expansion of human pancreatic islet-derived precursors

Rita Gallo, Fabio Arturo Grieco, Lorella Marselli, Elisabetta Ferretti, Alberto Gulino, Piero Marchetti, Francesco Dotta

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Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

A detailed understanding of the molecular process involved in the proliferation of pancreatic precursor cells would provide key elements for developing new therapeutic strategies to cure type 1 diabetes. In the present study we investigated the potential involvement of hedgehog signaling in proliferating human pancreatic islet-derived mesenchymal (hPIDM) cells, a population of cells that can be successfully expanded and induced to differentiate into an insulin-secreting phenotype. Here we report that in these precursor cells a hedgehog signaling pathway is activated, as shown by Gli1 expression, and that a dose-dependent inhibition of such a pathway by cyclopamine results in a significant reduction of cell proliferation.

Original language	English
Title of host publication	Annals of the New York Academy of Sciences
Pages	43-45
Number of pages	3
Volume	1150
DOIs	https://doi.org/10.1196/annals.1447.024
Publication status	Published - Dec 2008

Publication series

Name	Annals of the New York Academy of Sciences
Volume	1150
ISSN (Print)	00778923
ISSN (Electronic)	17496632

Keywords

Diabetes
Hedgehog signaling
Precursor cells

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1196/annals.1447.024

Cite this

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title = "Hedgehog signaling during expansion of human pancreatic islet-derived precursors",

abstract = "A detailed understanding of the molecular process involved in the proliferation of pancreatic precursor cells would provide key elements for developing new therapeutic strategies to cure type 1 diabetes. In the present study we investigated the potential involvement of hedgehog signaling in proliferating human pancreatic islet-derived mesenchymal (hPIDM) cells, a population of cells that can be successfully expanded and induced to differentiate into an insulin-secreting phenotype. Here we report that in these precursor cells a hedgehog signaling pathway is activated, as shown by Gli1 expression, and that a dose-dependent inhibition of such a pathway by cyclopamine results in a significant reduction of cell proliferation.",

keywords = "Diabetes, Hedgehog signaling, Precursor cells",

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year = "2008",

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T1 - Hedgehog signaling during expansion of human pancreatic islet-derived precursors

AU - Gallo, Rita

AU - Grieco, Fabio Arturo

AU - Marselli, Lorella

AU - Ferretti, Elisabetta

AU - Gulino, Alberto

AU - Marchetti, Piero

AU - Dotta, Francesco

PY - 2008/12

Y1 - 2008/12

N2 - A detailed understanding of the molecular process involved in the proliferation of pancreatic precursor cells would provide key elements for developing new therapeutic strategies to cure type 1 diabetes. In the present study we investigated the potential involvement of hedgehog signaling in proliferating human pancreatic islet-derived mesenchymal (hPIDM) cells, a population of cells that can be successfully expanded and induced to differentiate into an insulin-secreting phenotype. Here we report that in these precursor cells a hedgehog signaling pathway is activated, as shown by Gli1 expression, and that a dose-dependent inhibition of such a pathway by cyclopamine results in a significant reduction of cell proliferation.

AB - A detailed understanding of the molecular process involved in the proliferation of pancreatic precursor cells would provide key elements for developing new therapeutic strategies to cure type 1 diabetes. In the present study we investigated the potential involvement of hedgehog signaling in proliferating human pancreatic islet-derived mesenchymal (hPIDM) cells, a population of cells that can be successfully expanded and induced to differentiate into an insulin-secreting phenotype. Here we report that in these precursor cells a hedgehog signaling pathway is activated, as shown by Gli1 expression, and that a dose-dependent inhibition of such a pathway by cyclopamine results in a significant reduction of cell proliferation.

KW - Diabetes

KW - Hedgehog signaling

KW - Precursor cells

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U2 - 10.1196/annals.1447.024

DO - 10.1196/annals.1447.024

M3 - Conference contribution

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VL - 1150

T3 - Annals of the New York Academy of Sciences

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BT - Annals of the New York Academy of Sciences

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Hedgehog signaling during expansion of human pancreatic islet-derived precursors

Abstract

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Keywords

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