Hedgehog signaling during expansion of human pancreatic islet-derived precursors

Rita Gallo; Fabio Arturo Grieco; Lorella Marselli; Elisabetta Ferretti; Alberto Gulino; Piero Marchetti; Francesco Dotta

doi:10.1196/annals.1447.024

Hedgehog signaling during expansion of human pancreatic islet-derived precursors

Rita Gallo, Fabio Arturo Grieco, Lorella Marselli, Elisabetta Ferretti, Alberto Gulino, Piero Marchetti, Francesco Dotta

www.neuromed.it

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

A detailed understanding of the molecular process involved in the proliferation of pancreatic precursor cells would provide key elements for developing new therapeutic strategies to cure type 1 diabetes. In the present study we investigated the potential involvement of hedgehog signaling in proliferating human pancreatic islet-derived mesenchymal (hPIDM) cells, a population of cells that can be successfully expanded and induced to differentiate into an insulin-secreting phenotype. Here we report that in these precursor cells a hedgehog signaling pathway is activated, as shown by Gli1 expression, and that a dose-dependent inhibition of such a pathway by cyclopamine results in a significant reduction of cell proliferation.

Original language	English
Title of host publication	Annals of the New York Academy of Sciences
Pages	43-45
Number of pages	3
Volume	1150
DOIs	https://doi.org/10.1196/annals.1447.024
Publication status	Published - Dec 2008

Publication series

Name	Annals of the New York Academy of Sciences
Volume	1150
ISSN (Print)	00778923
ISSN (Electronic)	17496632

Keywords

Diabetes
Hedgehog signaling
Precursor cells

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1196/annals.1447.024

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Hedgehog signaling during expansion of human pancreatic islet-derived precursors. / Gallo, Rita; Grieco, Fabio Arturo; Marselli, Lorella; Ferretti, Elisabetta; Gulino, Alberto; Marchetti, Piero; Dotta, Francesco.

Annals of the New York Academy of Sciences. Vol. 1150 2008. p. 43-45 (Annals of the New York Academy of Sciences; Vol. 1150).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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abstract = "A detailed understanding of the molecular process involved in the proliferation of pancreatic precursor cells would provide key elements for developing new therapeutic strategies to cure type 1 diabetes. In the present study we investigated the potential involvement of hedgehog signaling in proliferating human pancreatic islet-derived mesenchymal (hPIDM) cells, a population of cells that can be successfully expanded and induced to differentiate into an insulin-secreting phenotype. Here we report that in these precursor cells a hedgehog signaling pathway is activated, as shown by Gli1 expression, and that a dose-dependent inhibition of such a pathway by cyclopamine results in a significant reduction of cell proliferation.",

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AU - Marselli, Lorella

AU - Ferretti, Elisabetta

AU - Gulino, Alberto

AU - Marchetti, Piero

AU - Dotta, Francesco

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N2 - A detailed understanding of the molecular process involved in the proliferation of pancreatic precursor cells would provide key elements for developing new therapeutic strategies to cure type 1 diabetes. In the present study we investigated the potential involvement of hedgehog signaling in proliferating human pancreatic islet-derived mesenchymal (hPIDM) cells, a population of cells that can be successfully expanded and induced to differentiate into an insulin-secreting phenotype. Here we report that in these precursor cells a hedgehog signaling pathway is activated, as shown by Gli1 expression, and that a dose-dependent inhibition of such a pathway by cyclopamine results in a significant reduction of cell proliferation.

AB - A detailed understanding of the molecular process involved in the proliferation of pancreatic precursor cells would provide key elements for developing new therapeutic strategies to cure type 1 diabetes. In the present study we investigated the potential involvement of hedgehog signaling in proliferating human pancreatic islet-derived mesenchymal (hPIDM) cells, a population of cells that can be successfully expanded and induced to differentiate into an insulin-secreting phenotype. Here we report that in these precursor cells a hedgehog signaling pathway is activated, as shown by Gli1 expression, and that a dose-dependent inhibition of such a pathway by cyclopamine results in a significant reduction of cell proliferation.

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Hedgehog signaling during expansion of human pancreatic islet-derived precursors

Abstract

Publication series

Keywords

ASJC Scopus subject areas

Access to Document

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