TY - GEN
T1 - Hedgehog signaling during expansion of human pancreatic islet-derived precursors
AU - Gallo, Rita
AU - Grieco, Fabio Arturo
AU - Marselli, Lorella
AU - Ferretti, Elisabetta
AU - Gulino, Alberto
AU - Marchetti, Piero
AU - Dotta, Francesco
PY - 2008/12
Y1 - 2008/12
N2 - A detailed understanding of the molecular process involved in the proliferation of pancreatic precursor cells would provide key elements for developing new therapeutic strategies to cure type 1 diabetes. In the present study we investigated the potential involvement of hedgehog signaling in proliferating human pancreatic islet-derived mesenchymal (hPIDM) cells, a population of cells that can be successfully expanded and induced to differentiate into an insulin-secreting phenotype. Here we report that in these precursor cells a hedgehog signaling pathway is activated, as shown by Gli1 expression, and that a dose-dependent inhibition of such a pathway by cyclopamine results in a significant reduction of cell proliferation.
AB - A detailed understanding of the molecular process involved in the proliferation of pancreatic precursor cells would provide key elements for developing new therapeutic strategies to cure type 1 diabetes. In the present study we investigated the potential involvement of hedgehog signaling in proliferating human pancreatic islet-derived mesenchymal (hPIDM) cells, a population of cells that can be successfully expanded and induced to differentiate into an insulin-secreting phenotype. Here we report that in these precursor cells a hedgehog signaling pathway is activated, as shown by Gli1 expression, and that a dose-dependent inhibition of such a pathway by cyclopamine results in a significant reduction of cell proliferation.
KW - Diabetes
KW - Hedgehog signaling
KW - Precursor cells
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UR - http://www.scopus.com/inward/citedby.url?scp=57749171363&partnerID=8YFLogxK
U2 - 10.1196/annals.1447.024
DO - 10.1196/annals.1447.024
M3 - Conference contribution
C2 - 19120265
AN - SCOPUS:57749171363
SN - 9781573317337
VL - 1150
T3 - Annals of the New York Academy of Sciences
SP - 43
EP - 45
BT - Annals of the New York Academy of Sciences
ER -