Helicobacter pylori releases a factor(s) inhibiting cell cycle progression of human gastric cell lines by affecting cyclin E/cdk2 kinase activity and Rb protein phosphorylation through enhanced p27kip1 protein expression

Patrizia Sommi, Monica Savio, Lucia A. Stivala, Claudia Scotti, Paola Mignosi, Ennio Prosperi, Vanio Vannini, Enrico Solcia

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Helicobacter pylori, the main cause of chronic gastritis, plays a central role in the etiology of peptic ulcer disease and gastric cancer. In vitro studies have shown that H. pylori increases gastric epithelial cell turnover, thus increasing the risk for the development of neoplastic clones. The mechanisms by which H. pylori promotes perturbation of cell proliferation are not yet elucidated. To investigate whether products released by H. pylori in culture media interfere with cell cycle progression of human gastric epithelial cells, four cell lines (MKN 28, MKN 7, MKN 74, and AGS) were incubated in the presence of H. pylori broth culture filtrate. Cell cycle analysis showed that a H. pylori-released factor(s) significantly inhibited the G1- to S-phase progression of MKN 28 and MKN 7 cell lines, with a reversible, nonlethal mechanism, independent of the expression of VacA, CagA, and/or urease. The cell cycle inhibition occurred concomitantly with an increase in p27KIP1 protein levels, a reduction in Rb protein phosphorylation on serine residues 807-811, and a significant decrease in cyclin E-associated cdk2 activity. In contrast, the cell cycle progression of MKN 74 and AGS cell lines was not affected by the H. pylori-released factor(s). In normal human fibroblasts, G1-phase cell accumulation was concomitant with the reduction in Rb protein phosphorylation; that, however, appeared to be dependent on p21WAF1/CIP1 rather than on p27KIP1 protein. A preliminary characterization showed that the molecular mass of the partially purified cell cycle inhibitory factor(s) was approximately 40 kDa. These results suggest that H. pylori releases a soluble factor(s) that may affect cell cycle progression of gastric epithelial cells through elevated levels of cdk inhibitor p27KIP1. This factor(s) might act in vivo on non-colonized distant cells, the most proliferating cells of human gastric mucosa.

Original languageEnglish
Pages (from-to)128-139
Number of pages12
JournalExperimental Cell Research
Volume281
Issue number1
DOIs
Publication statusPublished - 2002

Fingerprint

Cyclin-Dependent Kinase Inhibitor p27
Cyclin E
Retinoblastoma Protein
Helicobacter pylori
Stomach
Cell Cycle
Phosphotransferases
Phosphorylation
Cell Line
Epithelial Cells
Urease
G1 Phase
Gastritis
Gastric Mucosa
Peptic Ulcer
S Phase
Serine
Stomach Neoplasms
Culture Media
Clone Cells

Keywords

  • Cell cycle
  • Cyclin E
  • H. pylori
  • P27
  • Rb protein

ASJC Scopus subject areas

  • Cell Biology

Cite this

Helicobacter pylori releases a factor(s) inhibiting cell cycle progression of human gastric cell lines by affecting cyclin E/cdk2 kinase activity and Rb protein phosphorylation through enhanced p27kip1 protein expression. / Sommi, Patrizia; Savio, Monica; Stivala, Lucia A.; Scotti, Claudia; Mignosi, Paola; Prosperi, Ennio; Vannini, Vanio; Solcia, Enrico.

In: Experimental Cell Research, Vol. 281, No. 1, 2002, p. 128-139.

Research output: Contribution to journalArticle

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abstract = "Helicobacter pylori, the main cause of chronic gastritis, plays a central role in the etiology of peptic ulcer disease and gastric cancer. In vitro studies have shown that H. pylori increases gastric epithelial cell turnover, thus increasing the risk for the development of neoplastic clones. The mechanisms by which H. pylori promotes perturbation of cell proliferation are not yet elucidated. To investigate whether products released by H. pylori in culture media interfere with cell cycle progression of human gastric epithelial cells, four cell lines (MKN 28, MKN 7, MKN 74, and AGS) were incubated in the presence of H. pylori broth culture filtrate. Cell cycle analysis showed that a H. pylori-released factor(s) significantly inhibited the G1- to S-phase progression of MKN 28 and MKN 7 cell lines, with a reversible, nonlethal mechanism, independent of the expression of VacA, CagA, and/or urease. The cell cycle inhibition occurred concomitantly with an increase in p27KIP1 protein levels, a reduction in Rb protein phosphorylation on serine residues 807-811, and a significant decrease in cyclin E-associated cdk2 activity. In contrast, the cell cycle progression of MKN 74 and AGS cell lines was not affected by the H. pylori-released factor(s). In normal human fibroblasts, G1-phase cell accumulation was concomitant with the reduction in Rb protein phosphorylation; that, however, appeared to be dependent on p21WAF1/CIP1 rather than on p27KIP1 protein. A preliminary characterization showed that the molecular mass of the partially purified cell cycle inhibitory factor(s) was approximately 40 kDa. These results suggest that H. pylori releases a soluble factor(s) that may affect cell cycle progression of gastric epithelial cells through elevated levels of cdk inhibitor p27KIP1. This factor(s) might act in vivo on non-colonized distant cells, the most proliferating cells of human gastric mucosa.",
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